Transthyretin amyloidosis: a new mutation associated with dementia

NaCl-tolerant lactic acid bacteria (LAB) strains LC-10 (Lactobacillus casei) and LP-15 (Lact. plantarum) and NaCl were used as additives to sorghun (Sorghum bicolor). Numbers of LAB were significantly (P < 0.05) higher in all the additive-treated silages than in the control silage at an early stage of ensiling. During the fermentation process, addition of NaCl or LAB effectively inhibited the growth of aerobic bacteria and clostridia, but not yeasts. All the additive-treated silages had significantly (P < 0.05) lower pH, ammonia nitrogen content, dry matter loss and gas production but significantly (P < 0.05) higher lactic acid content and residual water soluble carbohydrates compared with the control silage. The improvement in silage quality was in the order: LAB > NaCl > control. Yeast counts were high in all additive-based silages and they increased during the exposure of the silages to air. As a result, these silages suffered aerobic deterioration, whereas the control silage was stable. The results confirmed that the NaCl or LAB improved fermentation quality but did not prevent aerobic deterioration of the silage.     

High-temperature sensible-heat storage options

Alkali-metal carbonate salts meet the requirements for high-temperature solar central receiver systems, but because of their corrosiveness they present special problems in the design of storage tanks. In order to reduce corrosion and temperature sufficiently to retain strength in the storage containing wall, internal and thermal insulation is required. We present design options and operation criteria for sensible-heat, molten-salt storage with internal insulation.     

High Throughput Primer Walking of cDNA Clones

Primer walking of cloned DNA is a standard research tool. It has been used in the past to determine the sequence of individual clones of interest. With the expansion of DNA sequencing capacity the need to be able to walk larger numbers of clones has become necessary. Our laboratory is a mid-sized genomics facility. In conjunction with the Advanced Biomedical Computing Center (ABCC) we have developed methods for automating the primer selection, DNA sequencing, contig assembly and sequence analysis for clones arrayed in microtiter format. This approach has allowed us to walk 475 clones (five microtiter plates) selected from a cDNA library.     

Synchrony effects in inhibitory control over thought and action.

Two experiments explore whether synchrony between peak circadian arousal periods and time of testing influences inhibitory efficiency for younger and older adults. Experiment 1 assesses inhibitory control over no-longer-relevant thoughts, and Experiment 2 assesses control over unwanted but strong responses, as well as performance on neuropsychological tasks that index frontal function. Inhibitory control is greatest at optimal times for both age groups and is generally greater for younger than for older adults. Performance on 2 neuropsychological measures (Stroop and Trails) also changes over the day, at least for older adults, and is correlated with inhibitory indexes, suggesting that for older adults changes in inhibition may be mediated by circadian variations in frontal functioning. By contrast, access to well-learned responses is not vulnerable to synchrony or age effects. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The performance of a direct-sequence spread-spectrum acquisition system using a linear prediction filter for narrowband interference suppression

The coarse acquisition performance of a direct sequence spread-spectrum receiver is analyzed when a linear prediction filter is used for narrowband interference suppression. We show that once an appropriate matching strategy is identified, the linear prediction filter can provide favorable performance when narrowband interference is present over a considerable range of both interference power and bandwidth. In addition, the presence of the filter dramatically improves the performance over the case where there is no filter, except when the interference bandwidth and the power are both small (i.e., when the processing gain provides sufficient interference immunity without the filter). If long spreading sequences are used with moderately sized observation windows, the acquisition performance can be severely degraded when a parallel acquisition scheme is used due to the linear predication filter. We show, however, that a slower serial receiver will provide reliable performance.This work was supported in part by the Office of Naval Research under contract ONR N00014-91-J-1234, the Army Research Office under contract ARO DAAL03-91-0071, and the NSF Center for Ultra-High Speed Circuits and Systems (ICAS).     

Effects of acetylcholine and glutamate on isolated neurons of locomotory network of Clione

In Clione limacina, locomotory rhythm is produced in the central pattern generator by reciprocal activity of two groups of interneurons. Dorsal (D) and ventral (V) phase interneurons activate neurons of the same phase and inhibit neurons of the opposite phase. Which neurotransmitters are used by these interneurons is not clear. In this study, identified follower neurons to V and D interneurons were isolated, and their responses to the local application of potential neurotransmitters were examined. Acetylcholine exerted inhibitory action on the isolated D-phase neurons and excitatory action on V-phase neurons. Glutamate produced excitation in D-phase neurons, and inhibition in V-phase neurons. These results suggest that acetylcholine is the neurotransmitter of D-phase interneurons, while glutamate might be the neurotransmitter of V-phase interneurons.     

Frequency discrimination: Assessing global-level and element-level units in memory.

Assessed the retention of memory units for word-like strings of letters using the perceptions of 120 undergraduates in 2 experiments in determining how frequently various letters appeared. A series of strings was presented at 1 of 3 exposure durations. Within the series, the frequencies of occurrence of different strings and of the letters composing the strings were varied orthogonally. Results indicate that, at relatively long exposure durations, Ss could discriminate the frequency of occurrence for both strings and their constituent letters. The formation of global-level (string) memory units was indicated by judgments of string frequency being unaffected by either the frequencies of their component letters or experimental conditions (brief exposures) that prohibited accurate judgment of letter frequency. Although judgments of letter frequency were sometimes biased by the frequency of the strings containing the letters, the success with which the judgments discriminated different levels of letter frequency did not depend on the activation of string-level memory units. Furthermore, Ss' frequency judgments for letters were not predictable from their recall of the strings containing the letters. Results provide evidence for the formation of element-level (letter) memory units. (21 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)