What Can We Learn from FGF-2 Isoform-Specific Mouse Mutants? Differential Insights into FGF-2 Physiology In Vivo

Fibroblast growth factor 2 (FGF-2), ubiquitously expressed in humans and mice, is functionally involved in cell growth, migration and maturation in vitro and in vivo. Based on the same mRNA, an 18-kilo Dalton (kDa) FGF-2 isoform named FGF-2 low molecular weight (FGF-2LMW) isoform is translated in humans and rodents. Additionally, two larger isoforms weighing 21 and 22 kDa also exist, summarized as the FGF-2 high molecular weight (FGF-2HMW) isoform. Meanwhile, the human FGF-2HMW comprises a 22, 23, 24 and 34 kDa protein. Independent studies verified a specific intracellular localization, mode of action and tissue-specific spatiotemporal expression of the FGF-2 isoforms, increasing the complexity of their physiological and pathophysiological roles. In order to analyze their spectrum of effects, FGF-2LMW knock out (ko) and FGF-2HMWko mice have been generated, as well as mice specifically overexpressing either FGF-2LMW or FGF-2HMW. So far, the development and functionality of the cardiovascular system, bone formation and regeneration as well as their impact on the central nervous system including disease models of neurodegeneration, have been examined. This review provides a summary of the studies characterizing the in vivo effects modulated by the FGF-2 isoforms and, thus, offers a comprehensive overview of its actions in the aforementioned organ systems.     

Parallel and scalable processing of spatio-temporal RDF queries using Spark

The ever-increasing size of data emanating from mobile devices and sensors, dictates the use of distributed systems for storing and querying these data. Typically, such data sources provide some spatio-temporal information, alongside other useful data. The RDF data model can be used to interlink and exchange data originating from heterogeneous sources in a uniform manner. For example, consider the case where vessels report their spatio-temporal position, on a regular basis, by using various surveillance systems. In this scenario, a user might be interested to know which vessels were moving in a specific area for a given temporal range. In this paper, we address the problem of efficiently storing and querying spatio-temporal RDF data in parallel. We specifically study the case of SPARQL queries with spatio-temporal constraints, by proposing the DiStRDF system, which is comprised of a Storage and a Processing Layer. The DiStRDF Storage Layer is responsible for efficiently storing large amount of historical spatio-temporal RDF data of moving objects. On top of it, we devise our DiStRDF Processing Layer, which parses a SPARQL query and produces corresponding logical and physical execution plans. We use Spark, a well-known distributed in-memory processing framework, as the underlying processing engine. Our experimental evaluation, on real data from both aviation and maritime domains, demonstrates the efficiency of our DiStRDF system, when using various spatio-temporal range constraints.

     

Efficient search based on content similarity over self-organizing P2P networks

The advent of the World Wide Web has made an enormous amount of information available to everyone and the widespread use of digital equipment enables end-users (peers) to produce their own digital content. This vast amount of information requires scalable data management systems. Peer-to-peer (P2P) systems have so far been well established in several application areas, with file-sharing being the most prominent. The next challenge that needs to be addressed is (more complex) data sharing, management and query processing, thus facilitating the delivery of a wide spectrum of novel data-centric applications to the end-user, while providing high Quality-of-Service. In this paper, we propose a self-organizing P2P system that is capable to identify peers with similar content and intentionally assign them to the same super-peer. During content retrieval, fewer super-peers need to be contacted and therefore efficient similarity search is supported, in terms of reduced network traffic and contacted peers. Our approach increases the responsiveness and reliability of a P2P system and we demonstrate the advantages of our approach using large-scale simulations.     

Effects of Excipients on Swelling and Drug Release from Compressed Matrices

Polymers, and particularly hydrogels, are becoming very popular in formulating controlled-release tablets because they are excellent drug carriers. The effects of hydrophilic and hydrophobic polymers, incorporated in matrices containing soluble (propranolol HCl) or less soluble (flurbiprofen) drugs, on swelling and release kinetics were investigated. The results indicate that swelling and release profiles were affected by the amount of ingredients, the characteristics of the polymer, and the drug substances incorporated in the matrices. Swelling may influence the release rate of the drugs from the matrices. The data obtained from the in vitro dissolution study were evaluated on the basis of a theoretical dissolution equation, by linear transformation of the dissolution curve, and by the Peppas equation. The release mechanisms appeared complex and are affected by the composition of the matrix     

Sliding wear and solid particle erosion response of aluminium reinforced with tungsten carbide nanoparticles and aluminide particles

In the present effort, aluminium matrix composites (AMCs) were produced by the addition of submicron‐sized WC particles of low (up to 2.5 vol%) content into a melt of Al1050. Casting was assisted by the use of K₂TiF₆ as a wetting agent and mechanical stirring in order to limit particle clustering. Particle distribution was reasonably uniform comprising both clusters and isolated particles. Various different reinforcing particles' phases were identified, both in situ (Al‐W, Al‐Ti, and Al‐W‐Ti intermetallic phases) and ex situ (WC particles) of various morphologies shapes and sizes. Increase of the reinforcing particle content led to an increase of the tendency for clustering. The wear properties of the composite were examined by dry sliding wear. The worn surfaces and the produced debris were examined by SEM‐EDX, and an effort to correlate the wear response of the produced materials with the matrix and the reinforcing phase characteristics was attempted. In general, the increase of the reinforcing phase content led to an improvement of the sliding wear response. Solid particle erosion experiments were carried out for impact angles of 30°, 60°, and 90°. Τhe eroded surfaces were examined with SEM‐EDX, and possible erosion mechanisms were proposed based on morphological and other material characteristics. Intensive particle clustering seemed to deteriorate the erosion resistance of the systems. Medium concentrations of the reinforcing particles (1.0‐1.5 vol% WC) are proposed as a recipe for optimum sliding wear and solid particle erosion resistance behavior.     

Electrophysiological Responses of Male Potato Cyst NematodesGlobodera rostochiensis and G. pallidato Some Chemicals

Electrophysiological techniques and behavioral bioassays were used to evaluate the responses of males of the potato cyst nematodesGlobodera rostochiensis and G. pallidato various chemicals. Exposure of all males of both species to acetylcholine elicited strong electrophysiological responses and positive movement towards the source. By contrast, neither glycine nor citric acid induced any marked electrophysiological responses and only males of G. pallida showed significant behavioral responses, moving toward glycine and away from citric acid. The electrophysiological and behavioral responses to GABA and α-aminobutyric acid were complementary, with G. rostochiensis showing significant response only to the latter and G. pallida responding significantly only to the former. The electrophysiological response of individuals of both species to L- and D-tryptophan varied from no response to a strong response with a significant mean increase in spike activity of G. rostochiensis to D-tryptophan and of G. pallida to L-tryptophan; behavioral bioassays showed L-tryptophan was a deterrent for G. pallida. Males of both species responded significantly in both electrophysiological and behavioral assays to L-glutamic acid but not to D-glutamic acid.     

Making sense of word senses: The comprehension of polysemy depends on sense overlap.

Studies of polysemy are few in number and are contradictory. Some have found differences between polysemy and homonymy (L. Frazier & K. Rayner, 1990), and others have found similarities (D. K. Klein & G. Murphy, 2001). The authors investigated this issue using the methods of D. K. Klein and G. Murphy (2001), in whose study participants judged whether ambiguous words embedded in word pairs (e.g., tasty chicken) made sense as a function of a cooperating, conflicting, or neutral context. The ambiguous words were independently rated as having low, moderate, or highly overlapping senses to approximate a continuum from homonymy to metonymic polysemy. The effects of meaning dominance were examined. Words with highly overlapping meanings (e.g., metonymy) showed reduced effects of context and dominance compared with words with moderately or low overlapping meanings (e.g., metaphorical polysemy and homonymy). These results suggest that the comprehension of ambiguous words is mediated by the semantic overlap of alternative senses/meanings. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Application of high-pressure treatment on ovine brined cheese: Effect on composition and microflora throughout ripening

Ovine brined cheese was high-pressure (HP) treated at 200 or 500 MPa for 15 min at 20 °C on the 15th day of ripening. Compared to control cheese, HP treatment did not affect significantly (P > 0.05) the pH values, moisture, fat in dry matter, protein in dry matter and salt in moisture contents of cheeses at 90 days. The counts of total aerobic mesophilic bacteria, thermophilic lactococci, thermophilic lactobacilli and non starter lactic acid bacteria (NSLAB) were not affected by HP treatment of cheese at 200 MPa throughout ripening. After 90 days of ripening, the same microbial groups in cheese treated at 500 MPa were about 1.2, 3.6, 2.1 and 4 log units lower than in control cheese respectively. Coliforms were reduced faster at non detectable levels in HP treated cheeses than in control cheese. Regarding the bacterial enzymatic activities in cheese, aminopeptidase activity (Apep) was marginally favoured by both HP treatments. However, its activity was decreased at 90 days due probably to loss in brine. In contrast, lactate dehydrogenase (LDH) activity, following the bacteria cell lysis, was negatively affected by HP treatment at 500 MPa throughout ripening.Industrial relevanceThe data obtained from this work suggest that application of HP treatment under optimized conditions on ovine cheese in brine can be used to reduce effectively the undesirable microbial load in it and to cause moderate enhancement of aminopeptidase activity, without modifying its composition.     

Electricity sector reform in Greece

This paper provides an outlook of the electricity market reform in Greece which started in 2001 and is still developing slowly. This is related to the persisting dominance of the incumbent company and the specificities of the electricity sector of Greece which is heavily dependent on indigenous lignite firing generation, while being located in the periphery of the EU internal electricity and gas markets. Competition through enhancing electricity trade in the region is limited to date, as the establishment of an internal market in South East Europe also progresses slowly. Development of competition through gas-firing generation by new entrants has been the priority adopted by State and Regulator's policies. However, the gas supply market in Greece and in the region still lags behind.     

Evolution of microbial populations during traditional Feta cheese manufacture and ripening

In three different dairies (A, B and C) located in Peloponess region (Southern Greece), traditional Feta cheese trials took place February to March using mixtures of sheep's and goat's milk. Only small variations in the evolution of microbial groups were observed during the whole ripening period. The main groups, such as thermophilic cocci, mesophilic lactococci, thermophilic lactobacilli, nonstarter lactic acid bacteria (NSLAB), presumptive Leuconostoc, enterococci and micrococci, reached their highest levels during the first 16 days, and then declined approximately 1-2 log units until the end of ripening. The remaining groups investigated, comprising yeasts, coliforms and Escherichia coli, were highest at day 4. The yeasts remained constant, while coliforms and E. coli decreased sharply and were not detectable after 120 days of ripening. A number of 146 isolates (dairy A) taken from all stages of the manufacturing and ripening process were purified and studied. Lactobacillus plantarum (58/146) and isolates of related species Lactobacillus pentosus and Lactobacillus paraplantarum (16/146) were the most common microorganisms found during cheese ripening. Streptococcus thermophilus (23/146) and Lactobacillus delbrueckii subsp. bulgaricus (20/146) were detected in high levels up to 20 days, and then gradually reduced. Enterococcus faecium (29/146) was found in all manufacturing and ripening stages.