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大鼠小肠嗜银、亲银细胞的分布及形态学观察 总被引:44,自引:3,他引:44
本文用肠卷石蜡切片的嗜银反应(黄荫乔法)、亲银反应(Singh法),对11只大鼠小肠的嗜银、亲银细胞的分布及形态学作了初步观察,结果如下。 1.大鼠小肠的嗜银、亲银细胞的密度,在十二指肠最高,从空肠到回肠逐渐减少。 2.嗜银、亲银细胞在肠腺基底部着色较浅,在腺上部着色加深,在绒毛顶端为深染。嗜银细胞基底部有突起,穿过基膜到达固有层。在固有层内,于突起附近有嗜银颗粒和突起相延续。嗜银颗粒到达细胞顶端较为多见,有时可见到嗜银颗粒释放到腺腔内。因此我们认为,嗜银、亲银细胞兼有内、外分泌双重功能。 3.在小肠固有层的结缔组织内,发现有嗜银细胞,细胞形状不规则,有突起,胞质和突起都充满嗜银颗粒,有时可见嗜银颗粒到达细胞外。颗粒的形态、致密度及染色特点,与上皮细胞之间的嗜银细胞相同,故这些细胞可能属于内分泌细胞。 相似文献
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豚鼠胰腺5—羟色胺免疫细胞化学定位 总被引:1,自引:0,他引:1
作者利用自制的5-羟色胺(5-HT)抗血清,成功地对豚鼠胰腺进行了免疫细胞化学定位。结果表明,应用PAP-GDN和PAP染色技术,观察到在豚鼠胰腺的内、外分泌部均有5-HT免疫反应细胞分布。在胰腺内分泌部(胰岛)5-HT免疫反应细胞分布均匀,形态多样,大小不等。在外分泌部,5-HT免疫反应细胞呈单个分散或聚集分布,主要位于腺上皮或导管上皮之间,或位于腺泡之间或导管旁的结缔组织之中。作者对其意义进行了讨论。 相似文献
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5-羟色胺(5-HT)为已知的辐射防护剂之一,文献已多有报道。 相似文献
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Immunocytochemical localization of 5-hydroxytryptamine (5-HT) containing cells of the guinea-pig pancreas was studied by means of PAP-glucose oxidase-DAB-nickel (PAP-GDN) and PAPstaining methods on paraffin sections. In this study we demonstrated the presence of 5-HTimmunoreactive cells in the endocrine (islets) and exocrine pancreas of the guinea-ping. Cells exhib-iting 54-HT immunoreactivity were found in the center and the periphery of the pancreatic islets. The5-HT immunoreactive granules were evenly distributed throughout the cytoplasm, but was faint inthe nuclei. Cells of this type were also distributed in the exocrine pancreas. Grouped or single or single cellsoccurred intercalatedly between acinar cells, as well as in the epithlium of ducts and in the connectivetissues near the ducts or acini. 相似文献
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