心肌梗死后移植骨髓间充质干细胞后全身细胞的分布

Objective To study the systemic distribution of bone marrow derived mesenchymal stem cells (MSCs) 24 h and 2 weeks after cell injection into the border zone of myocardial infarction area. Methods MSCs from male SD rats were labeled with Bromodeoxyuridine (BrdU). Three weeks after in-duction of myocardial infarction,female SD rats were randomized into 2 groups. Labeled cells (3 × 10 ,50 μl) were injected into the border zone of infarcted area in the one group (n = 12) ,and PBS of equal vol-ume was injected into the border zone of infarcted area in the other group ( n = 8 ). The systemic distribu-tion of MSCs was evaluated through real-time PCR and immunohistochemistry at time points of 24 h and 2 weeks after injection, respectively. Results Cells injected into border zone of infarcted area were distribu-ted to extra-cardiac organs such as spleen,lung and liver. Twenty-four h after injection,cells mainly con-centrated in the heart (467 467 ± 191 387) ,while obvious cell loss was noted in all organs including the heart ( 112 388 ±43 751 ) 2 weeks after injection. Of immunostaining were consistent with those of real-time PCR. Conclusion After injected into the border zone of infarcted area, MSCs mainly gathered in the heart with distributions into spleen, lung, and liver. However, substantial number of cells lost with pro-longed time span.     

SDF-1α在体内对骨髓间充质干细胞的保护作用及其对缺血心脏修复的影响

目的：研究基质细胞衍生因子1α（SDF-1α）在体内对移植干细胞的保护作用及能否提高干细胞移植对缺血心脏的疗效。方法：将用2μg/mL SDF-1α预处理过的干细胞与未处理组干细胞置于低氧无血清条件下6 h,用ELISA法检测VEGF的分泌。建立大鼠心梗模型,在梗死心肌与正常心肌边缘区注射50μL不含干细胞的IMDM（对照组,n=18）或含有3×106干细胞的IMDM（MSCs组,n=18）或含有3×106干细胞与2μg hSDF-1α的IMDM（SDF-1α＋MSCs组,n=18）。4 d后,用Western blot检测生存因子Bcl-2、磷酸化Akt4的表达,ELISA法检测VEGF及SDF-1α的表达量。4周后,接受心超检测、免疫组化染色。结果：SDF-1α蛋白能促进体内和体外环境下VEGF的分泌。Western blot证实经SDF-1α处理后,促生存因子Akt和Bcl-2表达增加。心梗后4周,相比单纯MSCs组而言,SDF-1α＋MCSs组能更多地促进新生血管的生成。但是在心功能方面,SDF-1α＋MCSs组和MSCs组没有明显差别。结论：SDF-1α能促进骨髓间充质干细胞的旁分泌作用,提高生存因子的表达,促进毛细血管新生,但未能加强干细胞移植对心功能的改善作用。     

左房黏液瘤合并二尖瓣返流的外科治疗

目的：总结术中发现二尖瓣返流的左房黏液瘤患者的相关临床资料,探讨其二尖瓣返流的临床特点、发生机制和处理方法。方法：2006年6月至2014年6月期间,因左房黏液瘤在温州医科大学附属第一医院行手术治疗的患者50例,其中切除肿瘤同期行二尖瓣手术的患者有10例。术者在切除肿瘤后常规对二尖瓣进行探查,术中应用食道心超对瓣膜情况进行评估,如发现存在中度以上的返流,则同期行二尖瓣成形术或者置换术。术后所有患者随访1年以上评估手术疗效。结果：10例同期行二尖瓣手术的患者在切除巨大肿瘤[长（5.0±1.9）cm,宽（4.1±1.0）cm]后发现均存在二尖瓣中重度返流,其中9例患者在术前检查中并未提示中度以上的二尖瓣返流。同期行二尖瓣成形患者9例,二尖瓣置换机械瓣1例,围手术期无死亡病例。随访期间无死亡、再次手术和肿瘤复发病例,未发现中度以上的二尖瓣返流。结论：对于行左房黏液瘤切除术的患者,特别是对于巨大黏液瘤的患者,在术中应警惕存在肿瘤切除后合并二尖瓣返流的可能,应在肿瘤切除后常规对二尖瓣进行探查,并且利用术中经食道心超及时动态地评估二尖瓣的功能情况,如发现存在严重的返流,推荐同期进行二尖瓣成形术。     

经静脉途径移植骨髓间充质干细胞在慢性心肌梗死大鼠体内的分布

背景:目前的研究中,大多针对急性心肌梗死进行细胞移植研究,而对于慢性心肌梗死后经静脉移植干细胞的研究尚少见,尤其是在移植后多个时间点动态监测移植的干细胞在体内分布及存活情况尚无报道.目的:观察经静脉途径移植骨髓间充质干细胞后在慢性心肌梗死模型大鼠体内的分布情况.方法:从雄性SD大鼠获取培养骨髓间充质干细胞,将18只雌性SD大鼠制备成心肌梗死3周后随机均分为2组,实验组:用胰岛素针抽取加入PBS的含有5×106骨髓间充质干细胞的混悬液300 μL,并将其缓慢注入股静脉;对照组:注入同等体积的PBS.另选9只雌性SD大鼠仅开胸,不结扎冠状动脉,作为假手术组,并将含有5×106骨髓间充质干细胞的混悬液300 μL通过股静脉注入体内.骨髓间充质干细胞移植24 h,2周及1个月后,各组大鼠体内的细胞分布情况应用实时荧光定量PCR技术进行检测.结果与结论:实验组与假手术组大鼠在细胞移植后1 d,肺组织、肝脏、脾脏中细胞分布差异无显著性意义(P > 0.05),而实验组心脏中细胞分布明显多于假手术组(P < 0.01);移植后2周,两组肺组织中细胞数目均急剧减少(P < 0.05),肝脏、脾脏、心脏中细胞分布差异无显著性意义(P > 0.05);移植4周后两组相比,肺组织、肝脏、脾脏中细胞分布差异亦无显著性意义(P > 0.05),而假手术组心脏中未检测到细胞.移植4周后,各组织中细胞分布均明显减少.在对照组中,各时间点均未检测到SRY基因.提示慢性心肌梗死经静脉移植骨髓间充质干细胞后,早期大量细胞滞留于肺组织,且细胞数目随时间延长锐减.此外,心脏,脾脏,肝脏中仍可有少量细胞分布.     

心肌梗死后移植骨髓间充质干细胞后全身细胞的分布

Objective To study the systemic distribution of bone marrow derived mesenchymal stem cells (MSCs) 24 h and 2 weeks after cell injection into the border zone of myocardial infarction area. Methods MSCs from male SD rats were labeled with Bromodeoxyuridine (BrdU). Three weeks after in-duction of myocardial infarction,female SD rats were randomized into 2 groups. Labeled cells (3 × 10 ,50 μl) were injected into the border zone of infarcted area in the one group (n = 12) ,and PBS of equal vol-ume was injected into the border zone of infarcted area in the other group ( n = 8 ). The systemic distribu-tion of MSCs was evaluated through real-time PCR and immunohistochemistry at time points of 24 h and 2 weeks after injection, respectively. Results Cells injected into border zone of infarcted area were distribu-ted to extra-cardiac organs such as spleen,lung and liver. Twenty-four h after injection,cells mainly con-centrated in the heart (467 467 ± 191 387) ,while obvious cell loss was noted in all organs including the heart ( 112 388 ±43 751 ) 2 weeks after injection. Of immunostaining were consistent with those of real-time PCR. Conclusion After injected into the border zone of infarcted area, MSCs mainly gathered in the heart with distributions into spleen, lung, and liver. However, substantial number of cells lost with pro-longed time span.     

糖尿病大鼠来源骨髓间充质干细胞体外培养观察及其生物学特性

目的：研究糖尿病是否会导致在体外培养条件下自体骨髓间充质干细胞的增殖、分泌和抗凋亡能力的改变。方法：利用链脲佐菌素（STZ）诱导制作成年大鼠糖尿病模型（n=6）,正常大鼠作为对照组（n=6）,分别于体外利用全骨髓贴壁法来扩增和纯化骨髓间充质干细胞后,取生长良好的第2代细胞作为本实验的研究对象。采用Cell Count kit-8（CCK-8）分别绘制两组细胞的生长曲线来评价增殖能力;采用酶联免疫吸附试验（ELISA）检测血管内皮生长因子（VEGF）和胰岛素样生长因子-1（IGF-1）的分泌量;应用Hoechst33342染色和膜联蛋白V/PI双染流式细胞术观察细胞在低氧无血清条件下的抗凋亡能力。结果：来源于糖尿病大鼠的骨髓间充质干细胞的增殖较正常的大鼠显著性减慢（P〈0.01）,在形态学上则表现为较正常的细胞更扁平。VEGF和IGF-1的分泌量较正常大鼠来源的明显降低,其中VEGF的分泌量为（80.7±13.1）pg/mL和（67.9±12.3）pg/mL（P〈0.05）,IGF-1的分泌量分别为（374.8±41.2）pg/mL和（281.8±26.8）pg/mL（P〈0.01）。在低氧无血清条件下,糖尿病来源的干细胞抗凋亡能力显著性降低,两组早期细胞凋亡指数分别为28.7±3.4和37.1±2.42（P〈0.01）。结论：糖尿病大鼠来源的骨髓间充质干细胞可以成功地在体外进行增殖培养,但是其在增殖能力、分泌、抗凋亡能力等一系列生物学性状上有不同程度的损害。     

经静脉途径移植骨髓间充质干细胞在慢性心肌梗死大鼠体内的分布

背景：目前的研究中，大多针对急性心肌梗死进行细胞移植研究，而对于慢性心肌梗死后经静脉移植干细胞的研究尚少见，尤其是在移植后多个时间点动态监测移植的干细胞在体内分布及存活情况尚无报道。 目的：观察经静脉途径移植骨髓间充质干细胞后在慢性心肌梗死模型大鼠体内的分布情况。 方法：从雄性SD大鼠获取培养骨髓间充质干细胞，将18只雌性SD大鼠制备成心肌梗死3周后随机均分为2组，实验组：用胰岛素针抽取加入PBS的含有5×106骨髓间充质干细胞的混悬液300 μL，并将其缓慢注入股静脉；对照组：注入同等体积的PBS。另选9只雌性SD大鼠仅开胸，不结扎冠状动脉，作为假手术组，并将含有5×106骨髓间充质干细胞的混悬液300 μL通过股静脉注入体内。骨髓间充质干细胞移植24 h，2周及1个月后，各组大鼠体内的细胞分布情况应用实时荧光定量PCR技术进行检测。 结果与结论：实验组与假手术组大鼠在细胞移植后1 d，肺组织、肝脏、脾脏中细胞分布差异无显著性意义(P > 0.05)，而实验组心脏中细胞分布明显多于假手术组(P < 0.01)；移植后2周，两组肺组织中细胞数目均急剧减少(P < 0.05)，肝脏、脾脏、心脏中细胞分布差异无显著性意义(P > 0.05)；移植4周后两组相比，肺组织、肝脏、脾脏中细胞分布差异亦无显著性意义(P > 0.05)，而假手术组心脏中未检测到细胞。移植4周后，各组织中细胞分布均明显减少。在对照组中，各时间点均未检测到SRY基因。提示慢性心肌梗死经静脉移植骨髓间充质干细胞后，早期大量细胞滞留于肺组织，且细胞数目随时间延长锐减。此外，心脏，脾脏，肝脏中仍可有少量细胞分布。     

心肌梗死后移植骨髓间充质干细胞后全身细胞的分布

Objective To study the systemic distribution of bone marrow derived mesenchymal stem cells (MSCs) 24 h and 2 weeks after cell injection into the border zone of myocardial infarction area. Methods MSCs from male SD rats were labeled with Bromodeoxyuridine (BrdU). Three weeks after in-duction of myocardial infarction,female SD rats were randomized into 2 groups. Labeled cells (3 × 10 ,50 μl) were injected into the border zone of infarcted area in the one group (n = 12) ,and PBS of equal vol-ume was injected into the border zone of infarcted area in the other group ( n = 8 ). The systemic distribu-tion of MSCs was evaluated through real-time PCR and immunohistochemistry at time points of 24 h and 2 weeks after injection, respectively. Results Cells injected into border zone of infarcted area were distribu-ted to extra-cardiac organs such as spleen,lung and liver. Twenty-four h after injection,cells mainly con-centrated in the heart (467 467 ± 191 387) ,while obvious cell loss was noted in all organs including the heart ( 112 388 ±43 751 ) 2 weeks after injection. Of immunostaining were consistent with those of real-time PCR. Conclusion After injected into the border zone of infarcted area, MSCs mainly gathered in the heart with distributions into spleen, lung, and liver. However, substantial number of cells lost with pro-longed time span.     

心肌梗死后移植骨髓间充质干细胞后全身细胞的分布

Objective To study the systemic distribution of bone marrow derived mesenchymal stem cells (MSCs) 24 h and 2 weeks after cell injection into the border zone of myocardial infarction area. Methods MSCs from male SD rats were labeled with Bromodeoxyuridine (BrdU). Three weeks after in-duction of myocardial infarction,female SD rats were randomized into 2 groups. Labeled cells (3 × 10 ,50 μl) were injected into the border zone of infarcted area in the one group (n = 12) ,and PBS of equal vol-ume was injected into the border zone of infarcted area in the other group ( n = 8 ). The systemic distribu-tion of MSCs was evaluated through real-time PCR and immunohistochemistry at time points of 24 h and 2 weeks after injection, respectively. Results Cells injected into border zone of infarcted area were distribu-ted to extra-cardiac organs such as spleen,lung and liver. Twenty-four h after injection,cells mainly con-centrated in the heart (467 467 ± 191 387) ,while obvious cell loss was noted in all organs including the heart ( 112 388 ±43 751 ) 2 weeks after injection. Of immunostaining were consistent with those of real-time PCR. Conclusion After injected into the border zone of infarcted area, MSCs mainly gathered in the heart with distributions into spleen, lung, and liver. However, substantial number of cells lost with pro-longed time span.     

心肌梗死后移植骨髓间充质干细胞后全身细胞的分布

Objective To study the systemic distribution of bone marrow derived mesenchymal stem cells (MSCs) 24 h and 2 weeks after cell injection into the border zone of myocardial infarction area. Methods MSCs from male SD rats were labeled with Bromodeoxyuridine (BrdU). Three weeks after in-duction of myocardial infarction,female SD rats were randomized into 2 groups. Labeled cells (3 × 10 ,50 μl) were injected into the border zone of infarcted area in the one group (n = 12) ,and PBS of equal vol-ume was injected into the border zone of infarcted area in the other group ( n = 8 ). The systemic distribu-tion of MSCs was evaluated through real-time PCR and immunohistochemistry at time points of 24 h and 2 weeks after injection, respectively. Results Cells injected into border zone of infarcted area were distribu-ted to extra-cardiac organs such as spleen,lung and liver. Twenty-four h after injection,cells mainly con-centrated in the heart (467 467 ± 191 387) ,while obvious cell loss was noted in all organs including the heart ( 112 388 ±43 751 ) 2 weeks after injection. Of immunostaining were consistent with those of real-time PCR. Conclusion After injected into the border zone of infarcted area, MSCs mainly gathered in the heart with distributions into spleen, lung, and liver. However, substantial number of cells lost with pro-longed time span.