AN UNUSUAL BILATERAL VAGAL PARAGANGLIOMAS： ONE CASE STUDY

VAGAL paraganglioma VP)is an uncommonneoplasm originating from neural crest paragan-glion cells located along the vagus nerve,repre-senting less than5%of all paragangliomas of the head andneck.1Despite improvement in microsurgical techniques,management of…     

差示分光光度法测定伤风止咳露中盐酸异丙嗪的含量

采用差示分光光度法测定伤风止咳露中盐酸异丙嗪的含量。以Ｈ２Ｏ２冰醋酸为氧化剂将盐酸异丙嗪氧化，测定氧化前后的吸收度差值。其△Ａ值与盐酸异丙嗪在一定浓度范围内呈线性关系。该法操作简便、快速、结果准确。     

Therapeutic effect of Shenfu Injection on secondary aplastic anemia of tumor patients after chemotherapy

Objective: To explore the effect of Shenfu Injection, SFI) on the secondary aplastic anemia (AA) of tumor patients after chemotherapy (CT).Methods: The 15 cases of SFI treated group, 10 cases of control group, 25 cases of SFI + granulocyte-macrophage colony stimulating factor (GMCSF) treated group and the group of 23 GM-CSF-treated tumor cases of secondary AA after CT were compared, with their increasing rate and the rebound speed of neutrophil, platelet, bone marrow nucleated RBC, granulocyte, and megakaryocyte all being investigated.Results: The increasing rate and rebound speed of granulocyte, platelet, and the increasing rate of bone marrow nucleated RBC, granulocyte, megakaryocyte were obviously higher than those of the control, and the clinical manifestations were also obviously improved. The increasing rate of platelet, bone marrow nucleated RBC, megakaryocyte of the SFI + GM-CSF group were higher than those of the group which used GM-CSF alone, while the increasing rate of granulocyte in blood and bone marrow in both groups was similar.Conclusion: Significant efficacy was shown in SFI for the treatment of secondary AA of tumor patients after CT.     

Identification of differentially expressed genes in omental adipose tissues of obese patients by suppression subtractive hybridization

To identify differentially expressed genes between obese individuals and normal control, we have undertaken suppression subtractive hybridization （SSH）. Omental adipose tissues were obtained via abdominal surgery for appendicitis in both 13 obese subjects[BMI （body mass index） 〉 30 kg/m（2）] and 13 normal subjects （BMI 〉 18 and 〈 25 kg/m（2））.     

子宫内膜癌手术范围的前瞻性临床研究

目的探讨子宫内膜癌的适宜手术范围、方法对105例子宫内膜癌患者施行广泛性或次广泛性子宫切除术,加盆腔及(或)腹主动脉旁淋巴结清扫术;另外36例子宫内膜癌患者仅作次广泛子宫切除术。对141例的手术切除标本,包括子宫旁结缔组织及淋巴结、盆腔及腹主动脉旁淋巴结等进行病理学检查。结果 105例子宫内膜癌患者中,盆腔淋巴结发生转移26例,占24．76％。除Ⅰa期外,其余各期均有淋巴结转移发生。Ⅰb、Ⅰc期淋巴结转移率分别为12．50％、21．05％。141例行广泛性或次广泛性子宫切除术者中,宫旁组织转移2例(Ⅱa、Ⅲb期),宫旁淋巴结转移2 例(Ⅰc、Ⅲb期);宫旁血管瘤栓2例(Ⅰb、Ⅰc期)。结论除Ⅰa期以外的其他各期,均宜施行广泛性或次广泛性子宫切除术,以及盆腔和／或腹主动脉旁淋巴结清扫术。     

好黄芩甙对发热家兔下丘脑及脑脊液中环磷腺芳含量的影响

目的：从中枢发热介质的角度探讨野黄芩甙的解热机制。方法：本实验用内毒素(ET)复制家兔发热模型，观察野黄芩甙的解热作用及对家兔下丘脑和脑脊液中环磷腺甙(cAMP)含量的影响。结果：静脉注射ET后80min，野黄芩甙 ET组家兔的体温明显低于ET组(P＜0.01)，表明野黄芩甙具有明显的解热作用；并且野黄芩甙能抑制ET所致的下丘脑中cAMP含量的升高(P＜0．01)和脑存液中cAMP含量的升高(P＜0．01)；相关分析显示，下丘脑和脑脊液中cAMP的含量变化与体温变化之间至明显的正相关(P＜0．01)。结论：野黄芩甙可能是通过抑制下丘脑和脑脊液中cAMP含量升高而发挥其解热作用的。     

Neuroprotective effect of estrogen after chronic spinal cord injury in ovariectomized rats

BACKGROUND: At present, there is still lack of effective drugs for chronic spinal cord injury, whereas it is found recently that estrogen has a neuroprotective effect on brain and spinal cord injuries. OBJECTIVE: To observe the effect of estrogen on the apoptosis of nerve cells after gradual chronic spinal cord injury in ovariectomized rats. DESIGN: A randomized controlled animal trial. SETTING: Institute of Orthopaedics, the Second Hospital of Lanzhou University. MATERIALS: Sixty-five female Wistar rats of common degree, weighing 220–250 g, were provided by the experimental animal center of Lanzhou University. The rats were randomly divided into sham-operated group (n =5), estrogen-treated group (n =30) and saline control group (n =30), and the latter two groups were observed at 1, 3, 7, 14, 28 and 60 days respectively, and 5 rats for each time point. METHODS: All the rats were treated with bilateral oophorectomy 2 weeks before the experiment. T10 vertebral lamina was revolved into using plastic screw. The spinal canal impingement was not induced initially. After that, the original incision was opened to expose the screw every 7–10 days. MAIN OUTCOME MEASURES: The apoptosis and Caspase-3 positive cells in the damaged spinal cord were detected using terminal deoxynucleotidal transferase-mediated dUTP-biotin nick end labeling (TUNEL) method and Caspase-3 immunohistochemical staining at 1, 3, 7, 14, 28 and 60 days after chronic spinal cord injury respectively. RESULTS: Totally 65 rats were used, and the deleted ones during the experiment were supplemented by others. Changes of Caspase-3 expression after spinal cord injury: In the sham-operated group, only a small amount of Caspase-3 proteins were observed in the rat spinal cord, mainly located in motor neurons of spinal cord anterior horn. In the estrogen-treated group and saline control group, positive cells expressed occasionally at 1 day postoperatively, began to increase obviously at 7 days after injury, strongly expressed at 14 and 28 days, but decreased at 60 days, mainly located in the neurons of spinal cord gray matter anterior horn, and they expressed fewer in the motor neurons and white matter of ventral horn, and there were obvious differences between the estrogen-treated group and saline control group at 7, 14, 28 and 60 days (P < 0.05). CONCLUSION: Estrogen can reduce the apoptosis of nerve cells and promote the recovery of neurological function following gradual chronic spinal cord injury.     

Small interfering RNA inhibits SARS-CoV nucleocapsid gene expression in cultured cells and mouse muscles

SARS-CoV isa newly identified coronavirus that causes severe acute respiratory syndrome （SARS）. Currently, there is no effective method available for prophylaxis and treatment of SARS-CoVinfections. In the present study, the influence of small interfering RNA （siRNA） on SARS-CoV nucleocapsid （N） protein expression was detected in cultured cells and mouse muscles. Four siRNA expression cassettes driven by mouse U6 promoter targeting SARS-CoV N gene were prepared, and their inhibitory effects on expression of N and enhanced green fluorescence protein （EGFP） fusion protein were observed.     

ENDOURETHRAL URETHROPLASTY WITH PREPUCE GRAFT FOR LONG POSTERIOR URETHRAL STRICTURE

Twenty patients underwent endourethral urethroplasty with free prepuce graft in combination with transurethral resection of scar for posterior urethral stricture. Two-year follow-up showed that they had good continent and free voiding results. Problems about surgical technique were detailed. We conclude that a free full-thickness prepuce evenly patched by means of the tube balloon to the freshly prepared wound derived from the stricture is the key to surgical success.