Whole CanA Gene Amplification of Helicobacter pylori and Its Fingerprinting by Restriction Fragment Length Polymorphism

To set up a method of amplification for the whole CagA gene of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism(RFLP),nested PCR was employed in combination with TD-PCR to amplify the gene and EcoRI and Hind Ⅲ were used to generate the RFLP fingerprinting.Target DNA fragments from 13 of 20 samples were successfully amplified and the relevant RFLP fingerprintings were obtained.It is concluded that the method can be used to amplify the whole CagA gene and CagA gene has apparent diversity of RFLP profile.     

甲硝唑棒治疗轻中度智齿冠周炎的临床疗效观察

目的:评价甲硝唑棒(牙康)对智齿冠周炎的治疗效果.方法:选择智齿冠周炎,张口受限Ⅰ度以内患者,并将患者随机分为甲硝唑棒治疗组(26例)和碘甘油对照组(25例),通过冠周盲袋冲洗后,治疗组放置甲硝唑棒,对照组放置碘甘油绵捻,隔日复诊,重复上述治疗,两组患者经6天上述治疗后观察两组的疗效.结果:治疗组治愈率为88.5%,对照组治愈率为48.0%,经χ2检验,P＜0.01,差异有统计学意义,甲硝唑棒治疗组治疗效果明显优于碘甘油.结论:甲硝唑棒用于智齿冠周炎的治疗,见效快、用药量少、局部药物浓度高、置药方法简便,疗效显著而值得临床推广应用.     

肝腺瘤1例误诊分析

1 临床资料 患者,女,19岁,我校化工系学生.因"间断性上腹部胀痛3天,转移至右下腹痛伴发热1天"前来就诊.查体:体温:38.0℃,腹平软,右下腹麦氏点有压痛,但无反跳痛及肌紧张.     

Whole Cag A Gene Amplification of Helicobacter pylori and Its Fingerprinting by Restriction Fragment Length Polymorphism

In recentyears,virulence factors of H elicobac-ter pylori ( Hp) have been focused on.In 1 988,Leunk first reported that Hp can produce a toxinleading to a vacuole- like pathology of eukareoticcells,called vacuole- associated cytotoxin A( Vac A) ,and found the toxin was produced in 5 5% of Hp[1] .In 1 990 ,Cover reported that anotherprotein encoded by a gene,cytotoxin- associatedgene A( cag A) ,is closely related to Vac A by us-ing immuno- blot[2 ] .Cag A has strong antigenicity.Ig G and …     

副距骨1例报告

患者,女,12岁.因发现右内踝部肿物1年,近来逐渐增大, 并时有麻木、疼痛感而入院.查体:右侧内踝部可扪及一2 cm×2.5 cm大小、质硬、无活动、表面光滑的肿物,有压痛,叩击肿物可引起放射性麻木并向右足内侧传导.X 线示右距骨内侧1 cm×1 cm骨性隆起,边缘光滑,无骨膜反应,左(-).     

新疆不同民族青年上颌前牙牙周生物型分布及相关因素分析

目的:本研究比较了不同牙位、民族的冠龈特征,并分析牙周生物型特征及其相关影响因素.方法:随机调查我国新疆石河子地区4个民族(汉族、维吾尔族、哈萨克族、回族)335名口腔健康人群的牙周生物型特征.通过临床和模型测量获得上颌前牙牙龈生物型(GB)、牙冠宽度(CW)、牙冠长度(CL)、牙冠宽长比(CW/CL)、附着龈宽度(AGW)和探诊深度(PD).据调查问卷评价GB与性别、种族、吸烟状况、饮酒、体重指数(BMI)、刷牙习惯及漱口水使用的相关性.结果:上颌前牙区不同牙位CW、CL、CW/CL、AGW存在显著差异;不同牙位的GB分布存在显著差异,中切牙、侧切牙和尖牙的厚龈型分别占60.1％、37.9％和26.1％(P<0.001).不同民族CL、CW/CL和AGW均存在差异(P<0.05).男性和少数民族(维族、哈族)厚龈型明显多于女性和汉族;吸烟、高BMI(18.5 kg/m2≤BMI<25 kg/m2,BMI≥25 kg/m2)人群侧切牙具有较多的厚龈型;饮酒、横纵刷牙方式结合的人侧切牙具有较多的薄龈型(P<0.05).结论:冠龈形态在不同牙位、种族在上有显著差异.且与GB相关的因素中,性别和民族在本研究中表现出更高相关性.     

应用铂类药物的新辅助化疗联合腹腔镜切除治疗T1期胃癌的临床效果观察

目的:研究铂类药物新辅助化疗联合腹腔镜切除治疗T1期胃癌的临床疗效.方法:临床纳入50例我院2013年9月~2015年5月收治的T1期胃癌患者作为研究对象,所有患者按入院顺序分为两组各25例.其中25例采用新辅助化疗联合开腹切除治疗作为对照组,另25例采用新辅助化疗联合腹腔镜切除治疗作为观察组.观察两组手术情况、术后恢复情况、并发症以及生存率.结果:观察组除手术时间比对照组长以及两组淋巴结清扫个数无差异外,手术出血量以及切口长度均优于对照组,P<0.05.观察组术后肛门排气时间、进食时间以及住院时间均短于对照组,P<0.05.观察组、对照组并发症发生率分别为8.0%、28.0%,观察组明显较低;观察组、对照组生存率分别为88.0%、72.0%,观察组明显较高,P<0.05.结论:铂类药物联合腹腔镜手术切除治疗对T1期胃癌治疗效果较好,术中创伤小,出血量少,并发症较少,生存率高,值得临床推广.