匹罗卡品致(癎)大鼠海马中表达生长抑素的中间神经元数目变化及其轴突出芽

Objective To investigate the roles of somatostatin(SS)positive intemeurons in the development and compensation of temporal lobe epilepsy.Methods Piloearpine-induced epilepsy rat model was established.Immunohistochemistry method was used to detect number changes and axonal sprouting of SS positive intemeurons in different domains of the hippocampus at difierent time points.Degeneration of SS positive interneurons and their neurophils were detected by the double immunofluorescence staining with SS and Fluoro-Jade B(FJB)at 7 and 60 days after status epilepticus (SE).Results In the exoerimental rat group,the number of SS positive neurons decreased in each hippocampal domain,and it reached the lowest at 7 days post-SE(There were 11.1±3.3 in hilus,2.8±0.9 in CA1region and 1.8±0.7 in CA1region,t=13.519,9.644 and 8.808,all P<0.01).In chronic phase,the number of SS neurons gradually recovered,and exceeded the control group in CA1 area at 60 days post-SE(12.8±1.5 vs 8.8±1.3,t=-4.506,P<0.01),however,the number of SS neurons in the hilus(25.5±4.6)and CA1 area(4.8±0.8)remained significantly less than normal levels(t value were 4.691 and 3.953.both P<0.01).Increased SS positive fibers were found in the lacunosum-molecular (1m)layer and outer molecular layer of dentate gyrus after 30 days post-SE,and numerous SS positive fibers were seen threnghout the layers of area CA1 at 60 days post-SE.Double immunofluuorescence revealed that a few SS positive interneurons and fibers were also labeled by FJB in area CA1 at 7 days post-SE and in CA domain/hilus at 60 days post-SE.Conclusions SS intemeurons loss plays an important role in the development of temporal lobe epilepsy.The loss is partially caIlsed by the degeneration and death of neurons;SS positive neurophils increase within area CA1 in chronic phase may play a significant role in the generation and compensation of temporal lobe epilepsy.     

中药促进透皮吸收作用研究近况

中药透皮给药制剂(外用)以其避免肝脏首过效应及胃肠道的毒副反应、长效、方便等独特的优势吸引着众多的国内外制剂研究者,并成为近年来国内外研究的热点.特别是欧美等国家对中药外用制剂及透皮吸收制剂的重视,使中药透皮给药系统的研究以及如何解决其药物对皮肤的穿透性和透皮速率等项研究更成为近年来国内外研究中药外用制剂的重点课题之一,本文就近年来促进中药透皮吸收作用的研究近况综述如下:     

匹罗卡品致(癎)大鼠海马中表达生长抑素的中间神经元数目变化及其轴突出芽

Objective To investigate the roles of somatostatin(SS)positive intemeurons in the development and compensation of temporal lobe epilepsy.Methods Piloearpine-induced epilepsy rat model was established.Immunohistochemistry method was used to detect number changes and axonal sprouting of SS positive intemeurons in different domains of the hippocampus at difierent time points.Degeneration of SS positive interneurons and their neurophils were detected by the double immunofluorescence staining with SS and Fluoro-Jade B(FJB)at 7 and 60 days after status epilepticus (SE).Results In the exoerimental rat group,the number of SS positive neurons decreased in each hippocampal domain,and it reached the lowest at 7 days post-SE(There were 11.1±3.3 in hilus,2.8±0.9 in CA1region and 1.8±0.7 in CA1region,t=13.519,9.644 and 8.808,all P<0.01).In chronic phase,the number of SS neurons gradually recovered,and exceeded the control group in CA1 area at 60 days post-SE(12.8±1.5 vs 8.8±1.3,t=-4.506,P<0.01),however,the number of SS neurons in the hilus(25.5±4.6)and CA1 area(4.8±0.8)remained significantly less than normal levels(t value were 4.691 and 3.953.both P<0.01).Increased SS positive fibers were found in the lacunosum-molecular (1m)layer and outer molecular layer of dentate gyrus after 30 days post-SE,and numerous SS positive fibers were seen threnghout the layers of area CA1 at 60 days post-SE.Double immunofluuorescence revealed that a few SS positive interneurons and fibers were also labeled by FJB in area CA1 at 7 days post-SE and in CA domain/hilus at 60 days post-SE.Conclusions SS intemeurons loss plays an important role in the development of temporal lobe epilepsy.The loss is partially caIlsed by the degeneration and death of neurons;SS positive neurophils increase within area CA1 in chronic phase may play a significant role in the generation and compensation of temporal lobe epilepsy.     

拓宽管理思路 促进科研整体水平提高

科学技术是第一生产力。市场条件下的竞争日趋激烈，其实质是科技与人才的较量。为了使中医科研的决策更具有科学性、先进性和可行性，笔者通过参与制定我院中医科研的全面规划，在调查研究和细致分析的基础上，就有关中医科研管理方面的想法试述如下。１着眼长远，制定中医科研的总体目标制定中医科研的总体规划，从发展规划、科研机构现状与预测的研究、中医事业战略研究等软科学研究等方面，为中医科研制定出切实可行的蓝图，使科研管理有遵循的目标与主攻方向。在指导思想上，以临床应用研究为主，遵循中医理论体系，坚持中医特色，探索…     

锁骨下静脉置管的护理体会

我科从2004年1月~2006年12月,对209例患者行锁骨下静脉置管,建立静脉输液通道、进行体液平衡监测及静脉营养治疗。此方法置管时间长,是抢救危重病人建立静脉通道较理想的方法。熟练的操作及精心护理可避免或减少并发症的发生,将护理体会总结如下。     

AB抗菌布杀菌作用的初步观察

ＡＢ抗菌布杀菌作用的初步观察冯莉，王小平，赖群元（江苏省常州市卫生防疫站２１３００３）ＡＢ抗菌布是一种新型抗菌保健织物。将无抗菌性能的纤维通过接技改性整理而使之具有永久性抗菌性能的卫生产品。我们于１９９４年９月在实验室内对其杀菌作用进行了初步观察。１...     

股静脉插管引起置管侧肢体肿胀分析及护理

男22例，女13例，年龄18～76岁，急性肾衰9例，其中挤压综合征合并急性肾功衰1例，坏死型胰腺炎并发急性肾功衰1例，肝肾综合征尿毒症1例。慢性肾衰26例，其中系统性红斑狼疮顽固性水肿1例，慢性肾炎肾功衰22例，糖尿病肾病3例，均采用单针双腔股静脉导管建立临时性血管通路。     

医学课程线上教学现状与启示——以中南大学湘雅医学院为例

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38例女童性早熟患者的性激素水平分析

近年来 ,随着人们生活水平的不断提高 ,儿童性早熟发病率明显升高 ,特别是女童性早熟。我们对我院自 1995年以来共收治的 38例女童性早熟患者进行了PRL、FSH、LH、E2含量分析 ,发现大多数性早熟女童属于假性性早熟 ,与下丘脑 -垂体 -性腺轴的变化无关 ,而只与单纯E2 升高有关。材料和方法一、标本来源 :(一 )正常对照组 :采集我院门诊 5～ 8岁无性早熟临床症状和体征的女童 30例血清作对照。(二 )性早熟女童组 :我院自 1995年收治的性早熟女童38例 (真性性早熟 9例 ,假性性早熟 2 9例 ) ,年龄 5～ 8岁 ,均经过B超、X线和其它临床检查证…     

绝经后阴道出血276例临床分析

绝经后阴道出血是指停经一年以上阴道出血,包括服用雌激素替代疗法时的不规则出血,是老年妇女常见症状之一,随着人口老龄化及人们保健意识的增强,绝经后阴道出血病人逐年增多,我院1995年1月－1999年12月5年中门诊共有绝经后阴道出血病人276人,现作分析如下。