Effects of penetrating cornea injury combined with seawater immersion on the levels of aqueous humor TNF-α in rabbits

Objective To observe effects of penetrating cornea injury combined with seawater immersion on the levels of aqueous humor TNF-α in rabbits. Methods 10 adult healthy grey rabbits were used for the experiment. The rabbit eye model of comea blast injury was developed by using a single firecracker. For each rabbit, the right eye was the experimental eye (seawater was injected into the anterior chamber through the cornea incision, simulating seawater immersion) and the left eye was the control (treated with 0.9% sodium chloride). Levels of aqueous humor TNF-α were detected by using double-antibody sandwich ELISA on the 1st, 2nd, yrd, 4th, 5th, and 7th days following seawater immersion or 0.9% sodium chloride injection. Results Following development of the model, levels of aqueous humor TNF-α in the experimental eye on the 1st, 2nd, 3rd days were 190.338, 156. 107,117.018 ng/L respectively, and significant differences could be seen when comparisous were made between the experimental and control eyes. The level of aqueous humor TNF-α on the 1st day was the highest and obviously reached the peak. Conclusions Level of aqueous humor TNF-α in the experimental eye with penetrating cornea injury combined with seawater immersion was significantly higher than that of the control eye, indicating that TNF-α was clearly involved in the pathological change and it played an important role in the process.     

眼眶骨折修复的人工材料的研究进展

眼眶骨折的治疗常需要植入修复材料,但修复材料的选择仍然是临床上一个争论性问题。目前有多种材料可以用于眶壁重建,但都有不同的并发症。与其他材料相比,人工材料更能缩短术中时间,并且用之不竭。其中可吸收人工材料还具有不可比拟的优势—即避免了不可吸收材料植入后终身都有发生并发症的风险。由于单个材料的优势有限,近年来相继出现的由两种或两种以上材料组成的复合材料开始频繁的应用于临床。本文旨在探讨用于眼眶骨折修复的各种人工材料的优势和局限性以及未来的发展方向。     

龟鹿补肾胶囊治疗100例肾气虚,肾阳虚证的临床报告

龟鹿补肾胶囊是由龟鹿补肾丸改变剂型而来。采用随机、对照原则，结果表明，龟鹿补肾胶囊治疗肾气虚、肾阳虚证的总有效率为９１％，显效率为３８％；龟鹿补肾丸总有效率为８４．３７％，显效率为２８．３１％。显示了龟鹿补肾胶囊优于龟鹿补肾丸的趋势。     

眼部结核2例

1病例报告例1:女,59岁,住院号304409,退休职员。患者发右上睑外侧包块1+mo,包块渐增大,无疼痛、复视、眼球动障碍,无发热、咳嗽、盗汗等。27a前因左眼新生血管青光眼,行左眼球摘除术。入院查体:右眼视力1.0,上外侧饱满,皮肤无明显充血,眼眶外上方触及条索状包块大小约1cm×0.5cm,后界不清,睑结膜面未触及,包块中,无明显压痛,眼球活动度正常,屈光介质透明,眼底见异常。左眼球缺如。颌下、耳前淋巴结未触及,心、肺腹部检查无明显阳性体征。眼眶CT报告:右侧泪腺区见不规则软组织密度影,与右侧眼球分界欠清晰,其内度尚均匀,CT值约32Hu,其大小约0.8cm×2cm,眶壁见骨质破坏。入院     

Effects of penetrating cornea injury combined with seawater immersion on the levels of aqueous humor TNF-α in rabbits

Objective To observe effects of penetrating cornea injury combined with seawater immersion on the levels of aqueous humor TNF-α in rabbits. Methods 10 adult healthy grey rabbits were used for the experiment. The rabbit eye model of comea blast injury was developed by using a single firecracker. For each rabbit, the right eye was the experimental eye (seawater was injected into the anterior chamber through the cornea incision, simulating seawater immersion) and the left eye was the control (treated with 0.9% sodium chloride). Levels of aqueous humor TNF-α were detected by using double-antibody sandwich ELISA on the 1st, 2nd, yrd, 4th, 5th, and 7th days following seawater immersion or 0.9% sodium chloride injection. Results Following development of the model, levels of aqueous humor TNF-α in the experimental eye on the 1st, 2nd, 3rd days were 190.338, 156. 107,117.018 ng/L respectively, and significant differences could be seen when comparisous were made between the experimental and control eyes. The level of aqueous humor TNF-α on the 1st day was the highest and obviously reached the peak. Conclusions Level of aqueous humor TNF-α in the experimental eye with penetrating cornea injury combined with seawater immersion was significantly higher than that of the control eye, indicating that TNF-α was clearly involved in the pathological change and it played an important role in the process.     

眼内炎眼球摘除术后Ⅱ期义眼植入术的临床分析

目的 探讨眼内炎眼球摘除术后羟基磷灰石义眼台Ⅱ期植入的临床应用疗效.方法 对25例(25眼)眼内炎眼球摘除术后患者,行Ⅱ期羟基磷灰石(HA)义眼植入术.手术采用直视下HA肌锥内植入法,并对临床效果进行回顾性分析.将患眼根据眼内炎的原因(外伤或感染)和眼球摘除后间隔时间进行分组,采用统计学分析义眼台暴露发生率和这些危险因素之间的关联.结果 5例患者(5只眼)出现伤口裂开、义眼台暴露,经修补治疗后得到解决,其余20例患者(20只眼)结膜均I期愈合.对23例患者(23只眼)进行了6个月至2年的随访,外形和活动度满意.外伤性眼内炎组义眼台暴露发生率(21.05%)和非外伤性眼内炎组发生率(16.67%)相比,无明显差异(P>0.05);感染性眼内炎组义眼台暴露发生率(33.33%)较非感染性眼内炎(0)组的发生率高(P<0.05);义眼台暴露发生率,在不同眼球摘除时间组无明显差异(P>0.05).结论 HA义眼Ⅱ期植入术,是眼内炎眼球摘除术后眼部成形的有效治疗方式,能有效改善外观,手术并发症少.     

63例老年眼附属器淋巴组织增生性疾病临床及病理分类研究

目的分析老年眼附属器淋巴组织增生性疾病的临床特点及病理分类。方法回顾性分析60岁以上眼附属器淋巴组织增生性疾病63例患者的临床、病理资料,并予以分类。结果63例眼附属器淋巴组织增生性疾病中反应性淋巴组织增生7例（11.1%）,不典型淋巴组织增生5例（7.9%）,淋巴瘤51例（81.0%）,各类型病变临床特征相似;51例眼附属器淋巴瘤中,有45例为黏膜相关淋巴组织边缘带B细胞淋巴瘤（MALT-EMZL）。结论老年眼附属器淋巴组织增生性疾病临床表现无特异性,大多数为低度恶性的淋巴瘤,病理检查是分类与鉴别诊断的主要依据,免疫组织化学技术能进一步明确诊断。     

Effects of penetrating cornea injury combined with seawater immersion on the levels of aqueous humor TNF-α in rabbits

Objective To observe effects of penetrating cornea injury combined with seawater immersion on the levels of aqueous humor TNF-α in rabbits. Methods 10 adult healthy grey rabbits were used for the experiment. The rabbit eye model of comea blast injury was developed by using a single firecracker. For each rabbit, the right eye was the experimental eye (seawater was injected into the anterior chamber through the cornea incision, simulating seawater immersion) and the left eye was the control (treated with 0.9% sodium chloride). Levels of aqueous humor TNF-α were detected by using double-antibody sandwich ELISA on the 1st, 2nd, yrd, 4th, 5th, and 7th days following seawater immersion or 0.9% sodium chloride injection. Results Following development of the model, levels of aqueous humor TNF-α in the experimental eye on the 1st, 2nd, 3rd days were 190.338, 156. 107,117.018 ng/L respectively, and significant differences could be seen when comparisous were made between the experimental and control eyes. The level of aqueous humor TNF-α on the 1st day was the highest and obviously reached the peak. Conclusions Level of aqueous humor TNF-α in the experimental eye with penetrating cornea injury combined with seawater immersion was significantly higher than that of the control eye, indicating that TNF-α was clearly involved in the pathological change and it played an important role in the process.     

6-氨基己酸静脉给药预防继发性前房出血的作用

为了提高手指疤痕挛屈曲畸形的手术效果，总结了7年来对236例836个志指行疤痕松解植皮或邻近皮瓣转移修复的经验．将手指挛缩分为轻、中、重三度．手术的关键在于避免损伤两侧的指动脉和指神经，不应一味追求完全伸直而影响手指血运。必要时分次进行，结合功能锻炼逐步达到较理想的效果．     

角膜穿通损伤合并海水浸泡模型免角膜组织病理学及房水白细胞介素6的变化

背景：由于海水特殊的理化性质,海上创伤后细胞的病理改变常有特殊性。目的：观察兔角膜爆炸穿通伤合并海水浸泡角膜组织病理学改变及房水白细胞介素6水平变化。方法：取成年健康灰兔16只,单支鞭炮致兔角膜爆炸伤,于角膜中周部做长3mm全层切口。右眼为实验侧,左眼为对照侧。实验侧将海水通过角膜切口注入前房,海水持续冲洗眼表30min。对照侧使用生理盐水。结果与结论：光镜观察显示实验侧造模后1、2和3d角膜上皮细胞大片坏死脱落,基质层水肿明显伴大量炎细胞浸润。对照侧1、2d角膜病理改变同实验组,但程度较轻,造模后3、5d局部病理改变明显减轻。实验组造模后第1、2、3d房水中自细胞介素6水平明显高于对照侧（尸〈0．05）。表明海水组伤情较对照组明显加重,提示海水可能是重要的致伤因素之一。