Slip Length Measurements Using µPIV and TIRF-Based Velocimetry

The goal of this paper is to review progress (mostly recent) made in micro and nanovelocimetry, focusing on two techniques: µPIV (microparticle image velocimetry) and nanoPTV (nanoparticle tracking velocimetry). The paper focuses on the measurement of slippage (taken as a benchmark for these techniques), concentrating on work done in our group. We review the developments of µPIV that led, in the last ten years, to the achievement of 100 nm accuracy in the measurement of slip lengths. Later, this approach was complemented by nanoPTV, which recently obtained ±5 nm precision. Here, we also mention recent application of these techniques toward better characterization of microgel and polymer flows. As a whole, the two techniques have conveyed valuable information on flow behavior within and close to the boundaries of microchannels, on the importance of wetting, and on the role of surface heterogeneities. µPIV is commercially available but nanoPTV is not mature. Interesting instrumental developments are expected in the future for the latter technique.     

Use of PCR-based methods and PFGE for typing and monitoring homofermentative lactobacilli during Comté cheese ripening

In order to obtain a better understanding of the biochemical events taking place in Saccharomyces cerevisiae during the lag phase, the proteins expressed during the first hours after inoculation were investigated by two-dimensional (2-D) gel electrophoresis and compared to those expressed in late respiratory growth phase. The studies were performed on a haploid strain (S288C) grown in defined minimal medium. Some of the abundant proteins, whose expression relative to total protein expression was induced during the lag phase, were identified by MALDI MS, and the expression of the corresponding genes was assessed by Northern blotting. The rate of protein synthesis was found to increase strongly during the lag phase and the number of spots detected on 2-D gels increased from 502 spots just after inoculation to 1533 spots at the end of the lag phase. During the first 20 min, the number of detectable spots was considerably reduced compared to the number of spots detected from the yeast in respiratory growth just prior to harvest and inoculation (747 spots), indicating an immediate pausing or shutdown in synthesis of many proteins just after inoculation. In this period, the cells got rid of most of their buds. The MALDI MS-identified, lag phase-induced proteins were adenosine kinase (Ado1p), whose cellular role is presently uncertain, cytosolic acetaldehyde dehydrogenase (Ald6p) and (DL)-glycerol-3-phosphatase 1, both involved in carbohydrate metabolism, a ribosomal protein (Asc1p), a fragment of the 70-kDa heat shock protein Ssb1, and translationally controlled tumour protein homologue (Yk1056cp), all involved in translation, and S-adenosylmethionine synthetase I involved in biosynthesis reactions. The level of mRNA of the corresponding genes was found to increase strongly after inoculation. By pattern matching using previously published 2-D maps of yeast proteins, several other lag phase-induced proteins were identified. These were also proteins involved in carbohydrate metabolism, translation, and biosynthesis reactions. The identified proteins together with other, yet unidentified, lag phase-induced proteins are expected to be important for yeast growth initiation and could be valuable biological markers for yeast performance. Such markers would be highly beneficial in the control and optimisation of industrial fermentations.     

Does a Cleaner Thames Pass an Economic Appraisal?

Abstract

Due to substantial clean-up efforts, water quality in the River Thames in London has steadily improved over the last three decades. However, London's Victorian sewage system still discharges untreated sewage into the Thames, at times of moderate to heavy rainfall. This happens approximately 60 times every year, creating adverse environmental and amenity impacts on the river. Major and costly engineering works will be required to solve the sewage overflow problem. This paper reports the results of a stated preference study designed to value the multiple non-market benefits—eductions in sewage litter, risk to human health, and risk to fish populations—associated with a range of potential engineering solutions. Our findings indicate that household willingness to pay for these benefits is likely to be significantly greater than the projected costs per household of, at least, some potential technical solutions. Although arguably justifiable on economic grounds, the final decision on whether to proceed with such a large infrastructure project is unlikely to depend wholly on cost-benefit thinking, but involve complex trade-offs with other socially desirable criteria.     

Qualifikation des Datenschutzbeauftragten durch praxisnahe Ausbildung

Die Anforderungen an Fachkunde und Aufgaben eines betrieblichen Datenschutzbeauftragten (DSB) sind hoch. Der Beitrag stellt die am vom Anforderungsprofil eines Datenschutzbeauftragten ausgerichteten Inhalte und Methoden der Ausbildung durch die GDD-Datenschutz-Akademie vor. Dort k?nnen Datenschutzbeauftragte zus?tzlich ihre Qualifikation zertifizieren.     

Linguistic Signatures of Regulatory Focus: How Abstraction Fits Promotion More Than Prevention.

In 3 experiments, the authors investigated how strategic inclinations associated with promotion versus prevention orientations--that is, eager approach versus vigilant avoidance, respectively--affect the use of language. It is hypothesized that eager promotion strategies used to attain desired end states entail using more abstract language than used with vigilant prevention strategies. This is shown to hold for experimentally induced relationship goals (Experiment 1) and communication goals (Experiment 2). In the 3rd experiment, the authors examined the impact of abstractly and concretely worded messages upon the behavioral intentions of chronically prevention- and promotion-oriented individuals and found support for the hypothesis that behavioral intentions to engage in specific activities are stronger when there is a fit between message wording and chronic orientation than when there is no fit. The broader implications of these findings are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The relationship between scientific and theoretical orientation among clinical psychology doctoral students.

Examined the relationship between theoretical orientation and research attitudes and interests among 233 doctoral students in clinical psychology. Responses to a survey sent to 10 doctoral training programs indicated that behaviorally oriented students are more interested in conducting research and are more likely to endorse the basic tenets of science than those students expressing a preference for psychodynamic or humanistic orientations. Behavioral students were also younger and more likely to have majored in psychology as undergraduates, suggesting an earlier commitment to a career in psychology. The results are discussed in terms of their implications for the role of training programs in encouraging clinical psychology graduate students to become better consumers of scientific literature. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Managing a hybrid energy smart grid with a renewable energy source

The aim of this work is to develop tools for optimal power flow management control in a micro grid (MG). The latter consists of a wind turbine, energy storage system, two gas turbines (GTs), and the main grid. Unlike the traditional approach, which is limited to the distribution of active power, this paper models an electrical system to coordinate and optimize the flow of both active and reactive power using discrete controls. The proposed optimal power distribution strategy has two objectives. First, it aims at forecasting over a time horizon of 24 hours the optimal distribution of the active and reactive power required for each power source connected to the MG. The proposed management incorporates the forecasts of consumption, weather, and tariffs. Second, it aims at reducing the CO₂ emissions rate by optimizing both the operating point of the two GTs and the usage of the storage unit. The proposed optimization problem for the energy management system is solved using the Bellman algorithm through dynamic programming. Its effectiveness is illustrated with various simulations carried out in the Matlab environment. The supremacy of the proposed management algorithm is highlighted by comparing its performance with conventional (restricted) management.     

A new PMU based power swing detector to prevent mal-operation of distance relay

In conventional transmission line protection, a distance relay is used to provide the primary as well as remote backup protection. The voltage and current phasors measurement needed by the distance relay for determining the impedance may be affected by the power disturbances such as power swing. Consequently, this power swing may cause mal-operation of Zone three distance relays which in turn may affect on the reliability of the whole protective scheme. To mitigate these effects and hence improve the relay reliability, this work proposes a new real-time power swing detector using phasor measurement units for blinding the distance relay only during this transient disturbance. However, this developed detector will not block relay when the power swing accompanied with faults. To validate the present work, the performance of developed enhanced distance relay is tested by signals generated by Simulink/MATLAB simulator under different conditions. The test results show that this proposed scheme provides good discrimination between the transient currents and the fault current which in turn it may contribute in enhancing the reliability of Distance relay.     

Adhesion of human pathogenic enteric viruses and surrogate viruses to inert and vegetal food surfaces

Enteric viruses, particularly human Noroviruses (NoV) and hepatitis A virus (HAV), are key food-borne pathogens. The attachment of these pathogens to foodstuff and food-contact surfaces is an important mechanism in the human contamination process. Studies were done to investigate the nature of the physicochemical forces, such as hydrophobic and electrostatic ones, involved in the interaction virus/matrix but, at this day, only few data are available concerning surface properties of viruses and prediction of the adhesion capacity of one specific virus onto matrices is still very difficult. The purpose of this study was to propose a reference system, including a representative virus surrogate, able to predict as close as possible behaviour of pathogenic viruses in term of adhesion on inert (stainless steel and polypropylene) and food surfaces (lettuce leaves, strawberries and raspberries). The adhesion of human pathogenic enteric viruses, cultivable strain of HAV and non-cultivable strains of human NoV (genogroups I and II), have been quantified and compared to these of human enteric viruses surrogates, included the MNV-1 and three F-specific RNA bacteriophages (MS2, GA and Qβ). A standardized approach was developed to assess and quantify viral adhesion on tested matrices after a contact time with each virus using real-time RT-PCR. Methods used for virus recovery were in accordance with the CEN recommendations, including a bovine Enterovirus type 1 as control to monitor the efficiency of the extraction process and amplification procedure from directly extracted or eluted samples. The adhesion of human pathogenic viruses, ranging from 0.1 to 2%, could be comparable for all matrices studied, except for NoV GII on soft fruits. Adhesion percentages obtained for the studied surrogate virus and phages were shown to be comparable to those of HAV and NoV on inert and lettuce surfaces. The MNV-1 appeared as the best candidate to simulate adhesion phenomena of all human pathogenic enteric viruses on all studied surfaces, while MS2 and GA bacteriophages could be a good alternative as model of viral adhesion on inert and lettuce surfaces. These results will be usable to design relevant experimental systems integrating adhesion behaviour of enteric viruses in the assessment of the efficiency of a technological or hygienic industrial process.