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In 2 daily experience studies and a laboratory study, the authors test predictions from approach-avoidance motivational theory to understand how dating couples can maintain feelings of relationship satisfaction in their daily lives and over the course of time. Approach goals were associated with increased relationship satisfaction on a daily basis and over time, particularly when both partners were high in approach goals. Avoidance goals were associated with decreases in relationship satisfaction over time, and people were particularly dissatisfied when they were involved with a partner with high avoidance goals. People high in approach goals and their partners were rated as relatively more satisfied and responsive to a partner's needs by outside observers in the lab, whereas people with high avoidance goals and their partners were rated as less satisfied and responsive. Positive emotions mediated the link between approach goals and daily satisfaction in both studies, and responsiveness to the partner's needs was an additional behavioral mechanism in Study 2. Implications of these findings for approach-avoidance motivational theory and for the maintenance of satisfying relationships over time are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
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The authors review commentaries by J. E. Broderick and A. A. Stone (2006)(see record 2006-03820-007); H. Tennen, G. Affleck, J. C. Coyne, R. J. Larsen, and A. DeLongis (2006)(see record 2006-03820-008); and M. K. T. Takarangi, M. Garry, and E. F. Loftus (2006)(see record 2006-03820-009) on their original article (A. S. Green, E. Rafaeli, N. Bolger, P. Shrout, & H. T. Reis, 2006)(see record 2006-03820-006). The authors were pleased to find more agreement than disagreement regarding the choice of methods for conducting diary studies. It is clear that continued critical evaluation of all diary methods, both paper and plastic, is warranted. However, on the basis of their initial findings, the authors conclude that paper diaries are still likely to have a valuable place in researchers' toolboxes. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
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The goals of the current study were to investigate the stability of temperamental exuberance across infancy and toddlerhood and to examine the associations between exuberance and social–emotional outcomes in early childhood. The sample consisted of 291 4-month-olds followed at 9, 24, and 36 months and again at 5 years of age. Behavioral measures of exuberance were collected at 9, 24, and 36 months. At 36 months, frontal electroencephalogram (EEG) asymmetry was assessed. At 5 years, maternal reports of temperament and behavior problems were collected, as were observational measures of social behavior during an interaction with an unfamiliar peer in the laboratory. Latent profile analysis revealed a high, stable exuberance profile that was associated with greater ratings of 5-year externalizing behavior and surgency, as well as observed disruptive behavior and social competence with unfamiliar peers. These associations were particularly true for children who displayed left frontal EEG asymmetry. Multiple factors supported an approach bias for exuberant temperament but did not differentiate between adaptive and maladaptive social–emotional outcomes at 5 years of age. (PsycINFO Database Record (c) 2011 APA, all rights reserved)  相似文献   
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BACKGROUND: Immunodominant epitopes of Bet v 1a had been identified before, using recombinant (r) Bet v 1a-reactive T cell clones generated from peripheral blood mononuclear cells of patients allergic to birch pollen. This study aimed at evaluating the T cell-stimulating capacity of immunodominant Bet v 1a-derived peptides in a polyclonal system corresponding more closely to the situation in patients. METHODS: Short-term T cell lines (TCL) were established in presence of a protein extract of birch pollen (BP extract). TCL proliferation induced by the BP extract, by natural Bet v 1, rBet v 1a, rBet v 2 or 5 selected immunodominant Bet v 1a-derived peptides was determined. RESULTS: Consistent with the knowledge that Bet v 1 is the major IgE-binding allergen of birch pollen, we found comparable T cell reactivity to natural Bet v 1 and the BP extract within the majority of the TCL. Accordingly, the response to rBet v 2 was low compared with the reactivity to the BP extract. The response of the TCL to rBet v 1a proved to be highly heterogeneous. Furthermore, the TCL response to the 5 immunodominant Bet v 1a-derived peptides showed considerable diversity. The proliferative responses of most TCL (with one exception) following stimulation by these peptides were low, in relation to the expansion induced by the BP extract. CONCLUSION: These findings argue against the use of selected peptides derived from Bet v 1a in specific immunotherapy of patients with birch allergy.  相似文献   
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Lanthanide (Ln)-dependent methanol dehydrogenases (MDHs) have recently been shown to be widespread in methylotrophic bacteria. Along with the core MDH protein, XoxF, these systems contain two other proteins, XoxG (a c-type cytochrome) and XoxJ (a periplasmic binding protein of unknown function), about which little is known. In this work, we have biochemically and structurally characterized these proteins from the methyltroph Methylobacterium extorquens AM1. In contrast to results obtained in an artificial assay system, assays of XoxFs metallated with LaIII, CeIII, and NdIII using their physiological electron acceptor, XoxG, display Ln-independent activities, but the Km for XoxG markedly increases from La to Nd. This result suggests that XoxG′s redox properties are tuned specifically for lighter Lns in XoxF, an interpretation supported by the unusually low reduction potential of XoxG (+172 mV). The X-ray crystal structure of XoxG provides a structural basis for this reduction potential and insight into the XoxG–XoxF interaction. Finally, the X-ray crystal structure of XoxJ reveals a large hydrophobic cleft and suggests a role in the activation of XoxF. These studies enrich our understanding of the underlying chemical principles that enable the activity of XoxF with multiple lanthanides in vitro and in vivo.  相似文献   
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Concern has been raised about the lack of participant compliance in diary studies that use paper-and-pencil as opposed to electronic formats. Three studies explored the magnitude of compliance problems and their effects on data quality. Study 1 used random signals to elicit diary reports and found close matches to self-reported completion times, matches that could not plausibly have been fabricated. Studies 2 and 3 examined the psychometric and statistical equivalence of data obtained with paper versus electronic formats. With minor exceptions, both methods yielded data that were equivalent psychometrically and in patterns of findings. These results serve to at least partially mollify concern about the validity of paper diary methods. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
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The authors used a vulnerability-stress-adaptation framework to examine personality traits and chronic stress as predictors of the developmental course of physical aggression in the early years of marriage. Additionally, personality traits and physical aggression were examined as predictors of the developmental course of chronic stress. Data from 103 couples collected 4 times over the first 3 years of marriage were analyzed with an actor-partner interdependence model and structural equation modeling techniques. Personality traits of husbands predicted their own physical aggression and stress trajectories, as well as their wives' levels of stress and physical aggression. Personality traits of wives predicted their levels of stress and physical aggression and predicted changes in their physical aggression over time. Both husbands' and wives' changes in stress predicted changes in physical aggression over time. Implications for employment of a vulnerability-stress-adaptation model in the study of physical aggression and for improvement of the efficacy of therapies targeting physical aggression in intimate relationships are delineated. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
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Evaluating collaborative systems remains a significant challenge. Most evaluation methods approach the problem from one of two extremes: focused evaluation of specific system features, or broad ethnographic investigations of system use in context. In this paper, we develop and demonstrate a middle ground for evaluation: explicit reflections on scenarios of system use coupled with analysis of the consequences of these use scenarios, represented as claims. Extending prior work in scenario-based design and claims analysis, we develop a framework for a multi-perspective, multi-level evaluation of collaborative systems called SWIMs: scenario walkthrough and inspection methods. This approach is centered on the capture, aggregation, and analysis of users’ reflections on system support for specific scenarios. We argue that this approach not only provides the contextual sensitivity and use centricity of ethnographic techniques, but also sufficient structure for method replication, which is common to more feature-based evaluation techniques. We demonstrate with an extensive field study how SWIMs can be used for summative assessment of system performance and organizational contributions, and formative assessment to guide system and feature re-design. Results from the field study provide preliminary indications of the method’s effectiveness and suggest directions for future research.  相似文献   
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