太赫兹辐射对小鼠脑神经发生及认知能力的影响

为探究太赫兹(THz)辐射对成年小鼠海马齿状回亚颗粒区(SGZ)神经发生及对老年小鼠认知能力的影响,用0.14 THz波照射实验小鼠头部,10 min/次,2次/d,连续21 d,对照组小鼠进行相同时间的麻醉。采用5-溴脱氧尿嘧啶核苷(BrdU)染色评价新生细胞存活、BrdU/神经元细胞核(NeuN)染色评价细胞分化、Morris水迷宫(MWM)实验分析认知能力变化。实验结果表明：与对照组相比,0.14 THz 10 min/次,2次/d,连续照射21 d后,可有效促进成年小鼠SGZ神经发生,成年小鼠SGZ新生细胞存活明显改善(P<0.05),新生细胞向神经元的分化无明显改变。老年小鼠逃避潜伏期,运动寻找轨迹无显著变化,THz辐射没有影响老年小鼠的空间认知能力。     

Systemic study on the safety of immuno-deficient nude mice treated by atmospheric plasma-activated water

Cold atmospheric-pressure plasma is a new technology,widely used in many fields of biomedicine,especially in cancer treatment.Cold plasma can selectively kill a variety of tumor cells,and its biological safety in clinical trials is also very important.In many cases,the patient's immune level is relatively low,so we first studied the safety assessment of plasma treatment in an immunocompromised animal model.In this study,we examined the safety of immuno-deficient nude mice by oral lavage treatment of plasma-activated water,and studied the growth status,main organs and blood biochemical indexes.Acute toxicity test results showed that the maximum dose of plasma treatment for 15 rain had no lethal effect and other acute toxicity.There were no significant changes in body weight and survival status of mice after 2 rain and 4 min of plasma-activated water (PAW) treatment for 2 weeks.After treatment,the major organs,including heart,liver,spleen,lung and kidney,were not significandy changed in organ coefficient and tissue structure.Blood biochemical markers showed that blood neutrophils and mononuclear cells were slightly increased,and the others remained unchanged.Liver function,renal function,electrolytes,glucose metabolism and lipid metabolism were not affected by different doses of PAW treatment.The above results indicate that PAW treatment can be used to treat immuno-deficient nude mice without significant safety problems.     

冷等离子体对肉蛋白的影响及其在肉品保藏加工中的应用研究进展

冷等离子体主要通过产生的活性氮或活性氧对肉品杀菌并降低内源酶活性,从而达到肉品保藏的目的,活性氮的存在使其可作为肉制品中亚硝酸盐的替代物,但活性粒子对肉品成分尤其是对蛋白质和脂肪的影响不容忽视,过度氧化最终导致肉品品质劣变。本文综述国内外关于冷等离子体技术在肉蛋白及肉品中的作用形式,及其对肉蛋白中肌原纤维蛋白和肌红蛋白结构和功能的调控作用及相关机制,并概述冷等离子体技术在肉品保藏加工领域的应用研究现状,以期为冷等离子体技术在肉品工业中的广泛应用提供参考。     

等离子体活化水溶液用于癌症治疗的研究综述

大气压冷等离子体作为高效产生外源活性粒子的新技术，很有可能成为安全、高效的癌症治疗新方法，但等离子体直接处理人体组织存在渗透深度浅、有效时间短的固有局限，使得应用潜力难以有效释放。等离子体活化水溶液（PAS）作为大气压冷等离子体的一种间接应用形式，具有活性时间长、穿透能力强和作用范围大等优势，近年来被广泛用于癌症治疗研究。该文主要围绕PAS制备、体外抗癌效应和生物学机制、活体抗癌应用及生物安全性三个层面，对近十年来的国内外相关研究进展进行了综述。PAS癌症治疗研究方兴未艾，该综述将为相关的理论和应用研究提供参考。