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Esteban Vera Pingitore María Silvina Juárez Tomás Birgitt Wiese 《Drug development and industrial pharmacy》2015,41(6):942-952
Context: The administration of pharmabiotics is a promising alternative to antimicrobial drugs for the treatment and/or prevention of female urogenital infections.Objective: To design pharmabiotic formulations including bioactive ingredients of microbial origin combined with non-microbial substances and then to evaluate the stability of the combinations during freeze-drying and storage.Materials and methods: Different formulations including Lactobacillus gasseri CRL 1263, Lactobacillus salivarius CRL 1328, salivaricin CRL 1328 (a bacteriocin) and non-microbial compounds (lactose, inulin and ascorbic acid) were assayed, and the ingredients were freeze-dried together or separately. The formulations were stored in gelatin capsules at 4?°C for 360?d.Results: The viability of lactobacilli was affected to different extents depending on the strains and on the formulations assayed. L. salivarius and ascorbic acid were successfully combined only after the freeze-drying process. Salivaricin activity was not detected in formulations containing L. gasseri. However, when combined with ascorbic acid, lactose, inulin or L. salivarius, the bacteriocin maintained its activity for 360?d. The selected microorganisms proved to be compatible for their inclusion in multi-strain formulations together with lactose, inulin and ascorbic acid. Salivaricin could be included only in a L. salivarius CRL 1328 single-strain formulation together with non-microbial substances.Conclusions: This study provides new insights into the design of urogenital pharmabiotics combining beneficial lactobacilli, salivaricin CRL 1328 and compounds with different functionalities. 相似文献
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Elucidation of colour development and microstructural characteristics of Allium sativum fumigated with acetic acid 下载免费PDF全文
Li Li Dan Wang Xihong Li Yu Wang Xiaofeng Ju 《International Journal of Food Science & Technology》2015,50(5):1083-1088
It has been well known that the greening of Allium sativum cloves could be formed after immersed in acetic acid solution. Nonetheless, no investigation was reported on colour development of A. sativum in response to acetic acid vapour until now. In this study, the brief exposure of A. sativum to acetic acid vapour (200–400 ppm) was combined with controlled atmosphere (5%, 20% and 80% CO2) packaging storage to break cell membrane and green garlic. The garlic bulbs were fumigated with acetic acid before controlled CO2 atmosphere packaging for 25 days at 4 °C. Fumigation with 200 ppm acetic acid followed by high CO2 atmosphere packaging (80% CO2) facilitated the garlic greening. It was also verified that γ‐glutamyl transpeptidase was involved in garlic greening in present study, and the compromise of glacial acetic acid vapour fumigation and CO2 gas atmosphere in package stored at low temperature could result in garlic greening as well. 相似文献
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宁东供水工程供水水泵共有卧式和立式离心泵2种方案可供选择,也是工程论证时争论的焦点,经综合比较及主机设备的国际招标,最终确定了奥地利安德里兹公司的卧式双吸中开式离心泵为宁东供水工程的供水水泵。 相似文献
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北部湾盆地福山凹陷CO2气成因探讨 总被引:2,自引:1,他引:1
北部湾盆地福山凹陷油气钻探中发现了高含CO2气的天然气气藏。对CO2气稳定碳同位素、伴生稀有气体氦和氩同位素进行了分析研究,结果显示,福山凹陷CO2气稳定碳同位素偏重,(13CCO2为-5.01~-10.08‰,绝大多数样品大于-7.0‰,为无机成因CO2气特征;伴生稀有气体氦同位素3He/4He值为(4.74~5.03)×10-6,R/Ra值为3.38~3.59;伴生稀有气体氩同位素40Ar/36Ar值为1881~2190,也显示出幔源或壳幔混合CO2气的特征。综合判定认为,福山凹陷CO2为壳幔混合成因。始新统流沙港组岩浆岩体分布特征与CO2气藏分布范围基本一致,也表明幔源-岩浆可能是福山凹陷CO2气的主要来源。与南海北部边缘盆地其它地区如珠江口盆地西部、琼东南盆地东部CO2气成因一致,都为幔源-岩浆来源,或壳幔混合来源。 相似文献
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Disciplinary and linguistic considerations for academic Web linking: An exploratory hyperlink mediated study with Mainland China and Taiwan 总被引:4,自引:0,他引:4
The Web has become an important means of academic information exchange and can be used to give new insights into patterns
of informal scholarly communication. This study develops new methods to examine patterns of university Web linking, focusing
on Mainland China and Taiwan, and including language considerations. Multiple exploratory investigations into Web links were
conducted between universities in these two places. Firstly, inlinks were counted to each university Web site from its national
peers using four alternative Web document models. The results were shown to correlate significantly with research productivity
in Taiwan but not in the Mainland, although in the latter case less reliable institutional data could have been the cause.
For Taiwan, this is the first evidence of a scholarly association with academic linking for a non-English speaking region.
It was then ascertained that the same link counts associated more strongly with scientific than social scientific research
productivity in Taiwan. This confirms the general assumption of greater Web use by the hard sciences. We then investigated
Taiwan-Mainland university cross-links, and found that although English is extensively used on the Web, there was no evidence
that it was the language of preference for informal scholarly communication between the two areas.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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Saehoon Ju Hyeongdong Kim Hyung-Hoon Kim 《Microwave and Wireless Components Letters, IEEE》2003,13(9):405-407
This letter presents a numerical dispersion relation for the two-dimensional (2-D) finite-difference time-domain method based on the alternating-direction implicit time-marching scheme (2-D ADI-FDTD). The proposed analytical relation for 2-D ADI-FDTD is compared with those relations in the previous works. Through numerical tests, the dispersion equation of this work was shown as correct one for 2-D ADI-FDTD. 相似文献
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In the present study, attempts are made to extend the application of the mechanical model for the fatigue crack initiation (FCI) and the FCI life formula of metallic notched elements in laboratory air to those in the corrosive environment. The test results and analysis of the corrosion FCI (CFCI) life of aluminum alloys and Ti---6A1---4V show that the expression of the CFCI life obtained by modifying the FCI life formula in laboratory air can give a good fit to the test results of the CFCI life. The salt water (3.5% NaCl) environment has no effects on the CFCI resistant coefficient compared with the FCI resistant coefficient in laboratory air. However, 3.5% NaCl environment greatly decreases the CFCI threshold of aluminum alloy, but has little effect on the CFCI threshold of Ti---6A1---4V. The loading frequency ranging from 1 Hz to 10 Hz has no appreciable effect on the CFCI life, and thus, the CFCI threshold of aluminum alloys investigated. Hence, the expression for the CFCI life of metallic notched elements proposed in this study is a better one, which reveals a correlation between the CFCI life and the governing parameters, such as, the geometry of the notched elements, the nominal stress range, the stress ratio, the tensile properties and the CFCI threshold. However, this new expression of the CFCI life needs to be verified by more test results. 相似文献