经口间断置入胃管管饲法的观察

临床上,鼻饲法是一种常用的护理支持方法,它是将胃管经一侧鼻腔插入胃内,从管内灌注食物、水和药物的方法[1],从而促进机体的正常代谢需要。而在实际中也有一些吞咽困难     

山楂叶总黄酮对慢性脑缺血大鼠p38MAPK信号通路的影响

目的探讨山楂叶总黄酮(TFHL)对慢性脑缺血大鼠脑组织p38丝裂原活化蛋白激酶(MAPK)信号通路的影响。方法健康雄性SD大鼠,随机分为假手术组、模型组、TFHL组、银杏叶片组。采用永久性双侧颈总动脉结扎法制备慢性脑缺血模型。Morris水迷宫检测大鼠学习记忆能力;Western印迹法检测p38蛋白的表达。结果与模型组比较,TFHL可明显改善血管性痴呆大鼠学习记忆能力,缩短大鼠逃避潜伏期(P<0.05),增加大鼠穿越平台次数(P<0.05),抑制大鼠缺血脑组织p38蛋白的表达(P<0.05)。结论 TFHL对慢性脑缺血大鼠的神经功能具有保护作用,其机制可能与抑制p38蛋白表达,减轻脑神经元凋亡有关。     

补阳还五汤对脑出血模型大鼠脑组织CXCR4-PI3K自噬信号传导通路及Beclin-1的影响

BACKGROUND:Buyang Huanwu Decoction is commonly used in clinical medicine in treatment of intracerebral hemorrhage. Previous studies have been found that it has excellent neuroprotective effect, can efficiently inhibit the apoptosis of nerve cells. Autophagic activity is closely related to apoptosis of nerve cells. CXCR4-PI3K autophagy signaling pathway has been verified in clinic. However, the effect of Buyang Huanwu Decoction is poorly understood. There is no study on Beclin-1 in the neuroprotective studies of Buyang Huanwu Decoction.  OBJECTIVE:To explore the effects of Buyang Huanwu Decoction on CXCR4-PI3K autophagy signaling pathway and Beclin-1 in rats with cerebral hemorrhage and related mechanisms.  METHODS:According to Rosenberg method, a rat model of cerebral hemorrhage was replicated and intragastrically administered Buyang Huanwu Decoction. Western blot assay was used to measure Beclin-1 protein. Immunohistochemical method was utilized to detect the expression of PI3K, AKT, stromal cell derived factor 1 and CXCR-4 protein. TUNEL assay was applied to identify apoptosis.  RESULTS AND CONCLUSION: (1) After administration, Buyang Huanwu Decoction could reduce the number of neuronal apoptosis in rats with intracerebral hemorrhage, up-regulate the expression of Beclin-1, PI3K, AKT, stromal cell derived factor 1, and CXCR-4 protein, and exert neuroprotective effect. (2) Buyang Huanwu Decoction could activate CXCR4-PI3K autophagy signal transduction pathway, both to stimulate autophagy and to regulate autophagy state, can inhibit apoptosis, and exert cerebral protective effect.     

自体脂肪移植的临床应用

近年来，随着脂肪抽吸术的广泛应用，自体脂肪颗粒注射移植术已在国内外得到重视与开展。自体颗粒脂肪移植以其无排斥、感染率低、注射后组织相容性好的优势，是目前较理想的填充材料。我们在汲取国内外研究及临床经验后，从2003年起进行了自体脂肪颗粒注射移植术，临床经验证明：自体脂肪注射对浅表凹陷的隆起、浅表微细皱纹的去除、薄唇增厚隆胸以及老年手部软组织萎缩的丰满等疗效确切。     

补阳还五汤提取物灌胃对脑出血大鼠脑组织中PI3K、AKT、Caspase-3表达的影响

目的 观察补阳还五汤提取物对脑出血大鼠脑组织磷脂酰肌醇3激酶(PI3K)、丝/苏氨酸蛋白激酶(AKT)、半胱氨酸天冬氨酸蛋白水解酶3(Caspase-3)表达的影响,探讨其对脑组织的保护作用机制。方法 将72只SD大鼠随机分为假手术组、模型组、补阳还五汤组、银杏叶片组各18只,后4组采用Rosenberg法建立脑出血大鼠模型后,分别给予生理盐水、补阳还五汤提取物、银杏叶片连续灌胃35 d。用免疫组化法检测PI3K、AKT、Caspase-3蛋白表达,TUNEL法检测脑组织细胞凋亡情况,干湿重法检测脑组织含水量,甲酰胺法检测血-脑脊液屏障通透性。结果 与模型组比较,补阳还五汤组、银杏叶片组PI3K、AKT蛋白表达水平升高,Caspase-3蛋白表达水平降低,脑组织含水量、凋亡细胞数减少,血-脑脊液屏障通透性降低(P均<0.05)。结论 补阳还五汤提取物可能通过激活PI3K/AKT信号通路,降低血-脑脊液屏障通透性来保护脑出血大鼠脑组织。     

补阳还五汤对脑出血模型大鼠脑组织PI3K、Akt、Bcl-2、BAX蛋白表达的影响

BACKGROUND:Buyanghuanwu decoction has excellent neuroprotective effect and can efficiently suppress nerve cell apoptosis caused by cerebral ischemia-reperfusion injury.