大鼠吻侧前扣带皮层的传入投射纤维联系——荧光金逆行追踪法研究

本文采用荧光金(FG)逆行束路追踪技术研究了大鼠前扣带皮层吻侧(rostralanteriorcingulatecortex,rACC)的传入投射纤维联系。将0.2μl的3%荧光金注入到大鼠单侧rACC,7d后灌注取材,将切片贴于载玻片上,在荧光显微镜下观察FG标记神经元的分布。FG标记神经元主要位于注射区同侧的许多皮层和皮层下结构,如丘脑中线核群和板内核群、杏仁核、次级视皮层、次级听皮层、外嗅皮层和嗅周皮层等。上述结果表明rACC不但接受来自丘脑的伤害性信息传入,也接受来自视、听、嗅皮层等的环境信息的传入。本研究的结果为rACC参与痛的情绪反应提供了形态学证据。     

在结核病防治工作中发现肺癌病人的探讨

肺癌又称支气管肺癌,是肺部最常见的恶性肿瘤.目前国内外肺癌的发病率都有逐年上升的趋势.北京市肺癌死亡已占癌症死亡病人的第一位,上海占第二位.石家庄市结核病防治所,十几年来在结核病防治工作中,提高对肺癌病的警惕,早期发现,早期诊断,指导肺癌的群防群治.1 在结核病流行病学调查中(包括健康检查)筛选肺癌病人石家庄市的结核病流行病学调查共检查了58725人,肺结核患病率为(633)/10万.同时检出肺癌病人5例,肺癌患病率为(8.5)/10万.可见X线胸部检查在发现肺结核的同时对肺癌的发现也具有重要     

NMDA和非NMDA受体参与介导猫脊髓内脏伤害性信息传递(英文)

目的:研究NMDA(N-methyl-D-aspartic acid)和非NMDA受体在介导脊髓内脏痛传入中的作用,方法:气球膨胀(3-15 kPa,20 s)麻醉猫结-直肠诱发脊髓背角痛敏神经元发放,结果:1)扩张结-直肠引起神经元发放增加的为兴奋性型:17个SLA型(短潜伏期突然增加);11个SLS型(短潜伏期渐增);9个LL型(长潜伏期),15个神经元属于抑制性的Inh型,2)67.6％,78.4％和59.5％的膨胀肠诱发兴奋的神经元,分别被微电泳NMDA、使君子酸(QA)和海人藻酸(KA)激活;60％,86.7％和53.3％的Inh神经元也分别被3个酸激活.3)微电泳NMDA受体拮抗剂d,l-2-amino-5-phosphonovalemte(APV)和非NMDA受体拮抗剂6,7-dinitro-quinoxaline-2,3-dione(DNQX),分别使兴奋性反应减少35％±10％和65％±14％,DNQX明显强于APV(P<0.05).DNQX使3/7个Inh神经元抑制翻转30％-50％,而APV无效,结论:NMDA和非NMDA受体均参与介导脊髓内脏伤害性信息传递,而非NMDA受体的作用更强。 (责任编辑 李颖)     

氯胺酮在吗啡急性耐受大鼠的外周镇痛作用

我们产证明氯胺酮引起的外周镇痛作用与阿片受体的激活有关。本实验旨在建立吗啡外周镇痛耐受的大鼠模型，进一步观察氯胺酮的外周镇痛作用与阿片受体的关系。外周感受野局部皮下注射５μｌ吗啡（１０μｇ／μｌ）明显的抑制伤害性肌电反应。随吗啡注射次数的增加，伤害性反应的抑制逐渐减弱，一般于第五次注射，吗啡不再产生抑制，出现外周镇痛的急性耐受。但是，在耐受动物的同一部位注射０μｌ氯胺酮（５０μｇ／μｌ），仍产生很     

Inhibition of P2X receptor-mediated inward current by protein kinase C in small-diameter dorsal root ganglion neurons of adult rats

Objective

The aim of the present study is to verify the ATP-induced varied responses in isolated dorsal root ganglion (DRG) neurons of the adult rat, and investigate the modulatory effects of specific P2X receptor agonist β, γ-me-ATP and protein kinase C (PKC) on P2X receptor-mediated inward current in DRG neurons.

Methods

Whole cell patch-clamp was employed to record the currents on acutely isolated DRG neurons in the adult rats.

Results

β, γ-me-ATP, similar as ATP, evoked 2 distinct subtypes of P2X receptor-mediated inward currents in a dose-dependent manner in DRG neurons. Activation of PKC by phorbol 12,13-dibutyrate (PDBu) significantly inhibited both subtypes of inward currents mediated by P2X receptors in a dose-dependent manner.

Conclusion

Activation of PKC negatively modulated the P2X receptor-mediated currents in rat DRG neurons, which may be of benefit to preventing the over-excitation of nociceptor under inflammatory or neuropathic conditions.     

NMDA受体介导皮下注射福尔马林诱发的大鼠脊髓Fos蛋白的表达

AIM: To examine the effects of N-methyl-D-aspartate (NMDA) and non-NMDA receptors on noxious stimulation-induced Fos expression in the rat spinal cord. METHODS: Formalin (2%) was injected s.c. into one hindpaw of the rat. Fos expression was exhibited by immunocytochemical technique. RESULTS: Two hours after s.c. formalin, Fos-like immunoreactive (FLI) neurons were distributed mainly in medial part of the lamina I and the outer lamina II of the ipsilateral dorsal horn. dl-2-Amino-5-phosphonovalerate (APV) administered intrathecally (10 microL, 0.01, 0.1, or 1 g.L-1) before injection of formalin into a hindpaw reduced the number of FLI neurons dose-dependently in the dorsal horn (P < 0.01), while 6,7-dinitroquinoxaline-2, 3(1H,4H)-dione (DNQX) (1 g.L-1) was ineffective. CONCLUSION: NMDA receptor mediated noxious stimulation-induced Fos expression in the rat spinal cord.     

NMDA和非NMDA受体参与介导猫脊髓内脏作害性信息传递

AIM: To study the role of N-methyl-D-aspartic acid (NMDA) and non-NMDA receptors in processing nociceptive visceral information in the spinal cord. METHODS: The firing of spinal dorsal horn neurons to colorectal distension (3-15 kPa, 20 s) by inflation with air of latex balloon was recorded in 25 anesthetized cats. RESULTS: 1) According to the patterns of responses to colorectal distension, the neurons with increase and decrease in firing were classified as excitatory and inhibitory, respectively. The former consisted of 17 short-latency abrupt (SLA) neurons, 11 short-latency sustained (SLS) neurons, 9 long-latency (LL) neurons. The 15 inhibited (Inh) neurons were recorded. 2) Microelectrophoretic administration of NMDA, quisqualic acid (QA), and kainic acid (KA) activated 67.6%, 78.4%, and 59.5% of the colorectal distension-excited neurons tested. Also, 60%, 86.7%, and 53.3% of Inh neurons were activated by these 3 amino acids. 3) Colorectal distension-induced excitatory responses were reduced by 35% +/- 10% and 65% +/- 14% by a selective NMDA receptor antagonist d,l-2-amino-5-phosphonovalerate (APV) and a selective non-NMDA receptor antagonist 6,7-dinitro-quinoxaline-2,3-dione (DNQX), respectively. Such DNQX-induced inhibition was significantly more potent than that by APV (P < 0.05). Colorectal distension-induced inhibitory responses were partially relieved by 30%-50% in 3/7 Inh neurons by DNQX, but not APV. CONCLUSION: Both NMDA and non-NMDA receptors are involved in transmission and/or modulation of spinal visceral nociceptive information and non-NMDA receptors may play more important role than NMDA receptors.     

静脉注射普鲁卡因对脊髓反射的影响

目的 研究静注射普鲁卡因对脊髓反射的影响,旨在探讨普鲁卡因注射产生镇痛是否发生在脊髓水平。方法 利用猫处周伤害性刺激能够引起的屈肌反射产生易化的模型,观察静脉注射普鲁卡因５和１０ｍｇ／ｋｇ对屈肌反射及屈肌反射及屈肌反射易化的影响。结果 ５ｍｇ／ｋｇ组屈肌反射及屈肌反射易化的抑制率分别为２０．５％（Ｐ〈０．０１,ｎ＝１２）和５３．３％（Ｐ〈０．０１,ｎ＝１２）,１０ｍｇ／ｋｇ组抑制率分别为５０．６％     

永记师恩

张先生的学生桃李满天下，在众多的弟子中，我是缺点最多、不大听话和无大作为的一个，但也是至今一直还工作在他身边的一个。从1962年进入张先生实验室之日起，在从师的35年中，无时无刻都感受到他的身教言教和亲切的关怀。从手把手教做实验到写文章，从处事到做人，是他领我步入科研之门，是他带我踏上脑科学研究的征程。在张先生九十华诞之际，作为弟子奉献的最好的生日礼物和对老师几十年教育的回报，莫过于丰硕的科研成果。很惭愧由于在科学上无大建树，拿不出象样的研究成果，我使他老人家失望了。好在师兄弟们的成就弥补了老师的缺憾…