多西环素对骨髓瘤细胞株H929内源性凋亡的影响及其作用机制

目的:探索多西环素(DOX)诱导骨髓瘤H929细胞株凋亡的作用及其可能机制.方法:用DOX、MEK抑制剂 U0126和 RAS 激动剂 ML-098单独或联合处理 H929细胞,Western blot 检测 p-MEK、caspase-3、caspase-9、c-Jun等蛋白的表达;CCK8法检测细胞增殖;Annex...     

CLINICAL AND EXPERIMENTAL STUDIES OF CHRONIC GASTRITIS PATIENTS WITH QI-DEFIClENCY OF SPLEEN

We have studied the syndrome characteris-tics of chronic gastritis with Oi-deficiency ofSpleen through observations on the effects ofdrugs that invigorate the Spleen and replenish thevital energy 健脾益气药 and on 4 indicators.The preliminary results are as follows.CLINICAL INVESTIGATIONClinical DataIn this series,the 41 patients were confirmedby gastroscopy and pathology to be chronicgastritis,some of them complicated by ulcers ofthe stomach or duodenal bulb.26 cases weremale and 15 cases female;the age ranged from     

多西环素通过MEK/ERK信号通路促进MC3T3-E1细胞株体外成骨分化

目的：研究在体外条件下多西环素（doxycycline,DOX）对前成骨细胞株MC3T3-E1成骨分化的影响及可能机制。方法：在成骨诱导剂体外诱导MC3T3-E1细胞株成骨分化条件下，茜素红染色检测成骨细胞分化；实时定量PCR检测DOX对MC3T3-E1细胞相关成骨基因OCN、Runx2的影响；用DOX、MEK抑制剂（U0126）单独或联合处理MC3T3-E1细胞后，Western blot检测N-cadherin、p-MEK及p-ERK等蛋白的表达。结果：在MC3T3-E1细胞株体外成骨诱导分化中，加入DOX可以增强茜素红染色阳性率。DOX上调MC3T3-E1细胞相关成骨基因OCN、Runx2的表达。DOX处理MC3T3-E1细胞后，其N-cadherin蛋白表达水平下降（P <0.05）,p-MEK和p-ERK蛋白的表达增加（P <0.05）。而MEK拮抗剂（U0126）则显著上调N-cadherin蛋白表达水平，同时降低p-MEK、p-ERK水平。用U0126联合DOX处理MC3T3-E1细胞后，DOX对N-cadherin、p-MEK和p-ERK蛋白水平的影响可被U...