Basal cell carcinoma stem cells exhibit osteogenic and chondrogenic differentiation potential

Specific cell subpopulations identified as cancer stem cells (CSCs) can be found in basal cell carcinoma (BCC). Generally, CSCs have a marked trans-differentiation potential that could potentially be used in differentiation therapies. However, there are no studies regarding BCC CSCs multipotency. The aim of the study was to analyze the characteristic of CSCs of BCC with emphasis on their differentiation potential upon specific induction. Specific staining and cell morphology were used for differentiation confirmation, along with the expression analysis of osteogenic (ALP, BSP, Runx2, OCN, BMP2), chondrogenic (COL1 and COL2A1), adipogenic (PPAR-γ) and neurogenic (Nestin and MAP2) markers. BCC CSCs differentiated into osteogenic and chondrogenic lineages, as judged by staining and high expression of specific markers (from 2-to 92-fold higher upon induction). Concomitantly with differentiation, the levels of cancer stem cell markers decreased in the cultures. Adipo-differentiation and neuro-differentiation were unsuccessful. In conclusion, BCC CSCs exhibit the capacity to trans-differentiate, a characteristic that may potentially be useful in the development of new strategies for the treatment of aggressive BCCs.     

Using communication to reduce locality in distributed multiagent learning

Abstract. This paper attempts to bridge the fields of machine learning, robotics, and distributed AI. It discusses the use of communication in reducing the undesirable effects of locality in fully distributed multi-agent systems with multiple agents robots learning in parallel while interacting with each other. Two key problems, hidden state and credit assignment, are addressed by applying local undirected broadcast communication in a dual role: as sensing and as reinforcement. The methodology is demonstrated on two multi-robot learning experiments. The first describes learning a tightly-coupled coordination task with two robots, the second a loosely-coupled task with four robots learning social rules. Communication is used to (1) share sensory data to overcome hidden state and (2) share reinforcement to overcome the credit assignment problem between the agents and bridge the gap between local individual and global group pay-off.     

STUDYING THE ROLE OF EMBODIMENT IN COGNITION

This paper raises the question of the connection between embodiment and higher level cognition, which has been eloquently addressed before but has not yet received much focus in the AI community. The paper then proceeds to break the question down into subparts and address how each can be approached and studied. Finally, the paper briefly overviews two directions of our work, group behavior and imitative behavior, and describes their relation to the issue of embodiment and cognition.     

Erythrophagosomal haemolytic degradative pathway in rat brown adipocytes induced by hyperinsulinaemia: an ultrastructural study

The phagocytosis and degradation of erythrocytes were studied in brown adipose tissue of experimentally hyperinsulinaemic rats. We found that insulin induces intensive erythrophagocytosis by brown adipocytes and their degradation by haemolytic pathway. Ultrastuctural study revealed that haemolytic degradation of erythrophagosomes was characterized by progressive and uniform decrease of erythrocyte matrix density. At the beginning of the degradative process small, clear vesicles resembling primary lysosomes were visible inside the erythrophagosome. With time, the erythrocyte structure totally disappeared and transformed into a fine, granular material within the erythrophagosomal vacuole. Finally, the erythrocyte membrane detached from the phagosomal and clumped into the vacuolar space forming one or several small myelin‐like figures. In conclusion, brown adipocytes are capable of performing intensive erythrophagocytic activity when brown adipose tissue is stimulated and blood flow is enhanced. The molecular basis for favouring a haemolytic instead of more common granular erythrophagosomal degradative pathway remains unknown.