全文获取类型
收费全文 | 359篇 |
免费 | 33篇 |
国内免费 | 3篇 |
学科分类
医药卫生 | 395篇 |
出版年
2022年 | 8篇 |
2021年 | 6篇 |
2020年 | 3篇 |
2019年 | 4篇 |
2018年 | 3篇 |
2017年 | 7篇 |
2016年 | 3篇 |
2015年 | 12篇 |
2014年 | 8篇 |
2013年 | 12篇 |
2012年 | 12篇 |
2011年 | 12篇 |
2010年 | 7篇 |
2009年 | 15篇 |
2008年 | 13篇 |
2007年 | 11篇 |
2006年 | 9篇 |
2005年 | 10篇 |
2004年 | 5篇 |
2003年 | 8篇 |
2002年 | 10篇 |
2001年 | 11篇 |
2000年 | 15篇 |
1999年 | 11篇 |
1998年 | 23篇 |
1997年 | 18篇 |
1996年 | 15篇 |
1995年 | 6篇 |
1994年 | 14篇 |
1993年 | 7篇 |
1992年 | 8篇 |
1991年 | 4篇 |
1990年 | 10篇 |
1989年 | 5篇 |
1988年 | 8篇 |
1987年 | 4篇 |
1986年 | 6篇 |
1985年 | 3篇 |
1983年 | 4篇 |
1982年 | 5篇 |
1980年 | 2篇 |
1979年 | 7篇 |
1978年 | 4篇 |
1977年 | 2篇 |
1976年 | 8篇 |
1975年 | 3篇 |
1974年 | 5篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有395条查询结果,搜索用时 15 毫秒
1.
Arbour NC; Zlotogora J; Knowlton RG; Merin S; Rosenmann A; Kanis AB; Rokhlina T; Stone EM; Sheffield VC 《Human molecular genetics》1997,6(5):689-694
Achromatopsia is an autosomal recessive disease of the retina,
characterized clinically by an inability to distinguish colors, impaired
visual acuity, nystagmus and photophobia. A genome-wide search for linkage
was performed using an inbred Jewish kindred from Iran. To facilitate the
genome-wide search, we utilized a DNA pooling strategy which takes
advantage of the likelihood that the disease in this inbred kindred is
inherited by all affected individuals from a common founder. Equal molar
amounts of DNA from all affected individuals were pooled and used as the
PCR template for short tandem repeat polymorphic markers (STRPs). Pooled
DNA from unaffected members of the kindred was used as a control. A
reduction in the number of alleles in the affected versus control pool was
observed at several loci. Upon genotyping of individual family members,
significant linkage was established between the disease phenotype and
markers localized on chromosome 2. The highest LOD score observed was 5.4
(theta = 0). When four additional small unrelated families were genotyped,
the combined peak LOD score was 8.2. Analysis of recombinant chromosomes
revealed that the disease gene lies within a 30 cM interval which spans the
centromere. Additional fine-mapping studies identified a region of
homozygosity in all affected individuals, narrowing the region to 14 cM. A
candidate gene for achromatopsia was excluded from this disease interval by
radiation hybrid mapping. Linkage of achromatopsia to chromosome 2 is an
essential first step in the identification of the disease-causing gene.
相似文献
2.
3.
This long-term follow-up study examined patients with chronic inflammatory demyelinating polyneuropathy (CIDP) and only sensory
symptoms at first presentation, with emphasis on the development of motor symptoms and long-term disability. From all CIDP
patients referred to our Department between 1987 and 1995, seven had only sensory symptoms at first clinical presentation.
These were investigated according to a standard protocol, including a quantified clinical neurological examination and nerve
conduction studies. The mean duration of the disease before weakness developed was 3.1 years, but varied considerably (0.8–6.3
years). At follow-up, weakness developed in five patients and persisted in three of them. Five patients were not seriously
incapacitated by their disease (Rankin 1 or 2), four of them being in remission now and one showing a very slow progression
of disease. Two patients were moderately disabled (Rankin 3); one had severe persistent sensory ataxia and only weakness during
relapses and one had stepwise progression and moderate weakness. Motor nerve conduction studies revealed that the most notable
worsening in the entire group of patients was a decrease in distal compound muscle action potential amplitudes, indicating
the development of distal conduction block or axonal degeneration. These findings show that CIDP with only sensory symptoms
is a transient clinical stage that precedes the appearance of weakness in about 70% of patients. The long-term prognosis does
not differ from that of patients with CIDP who have weakness at the beginning of the disease.
Received: 3 December 1998 Received in revised form: 17 May 1999 Accepted: 2 July 1999 相似文献
4.
5.
Romy D Zwittink Cornelia CH Wielders Daan W Notermans Nelianne J Verkaik Annelot F Schoffelen Sandra Witteveen Varisha A Ganesh Angela de Haan Jeroen Bos Jacinta Bakker Caroline Schneeberger-van der Linden Ed J Kuijper Sabine C de Greeff Antoni PA Hendrickx 《Euro surveillance : bulletin européen sur les maladies transmissibles = European communicable disease bulletin》2022,27(50)
Since March 2022, there has been an emergence of multidrug-resistant organisms (MDRO) in the Netherlands in patients originating from Ukraine (58 patients, 75 isolates). For about half of these patients, recent hospitalisation in Ukraine was reported. Genomic surveillance revealed that the majority of the MDRO represent globally spread epidemic lineages and that 60% contain New Delhi metallo-β-lactamase (NDM) genes. Professionals should be aware of an increase in such MDRO associated with migration and medical evacuation of people from Ukraine. 相似文献
6.
7.
In vivo expression of the B7 costimulatory molecule by subsets of antigen-presenting cells and the malignant cells of Hodgkin's disease 总被引:10,自引:0,他引:10
Munro JM; Freedman AS; Aster JC; Gribben JG; Lee NC; Rhynhart KK; Banchereau J; Nadler LM 《Blood》1994,83(3):793-798
The B-lymphocyte/accessory-cell activation antigen B7 (BB1) has been shown in vitro to stimulate T-lymphocyte proliferation and cytokine production via CD28 present on the latter cells. In this study, benign lymphoid tissues, lymphomas, and extralymphoid inflammatory sites were examined immunohistochemically using anti-B7 and other relevant monoclonal antibodies. B7 was expressed by benign transformed germinal center B cells, as it was by B cells of follicular lymphomas. B7 was also expressed by a subpopulation (a mean of 31% to 65%) of macrophages and dendritic cells in a variety of lymphoid tissues. It was present in abundance on all macrophages constituting sarcoid granulomas in lymph nodes. In extralymphoid inflammation, 17% to 35% of macrophages expressed B7 only weakly. Cases of Hodgkin's disease showed expression of B7 by the majority of Reed-Sternberg cells or malignant mononuclear variants, a phenomenon that potentially contributes to the lymphocytic accumulation that is a feature of this condition. CD28+ T cells were seen in all areas where T cells were present. B7+ and CD28+ cells colocalized in, for example, lymphoid follicles, lymph node paracortex, sarcoid granulomas, and Hodgkin's disease tissue, indicating a potential for cellular interaction via these molecules at these sites. 相似文献
8.
Foudraine NA Hovenkamp E Notermans DW Meenhorst PL Klein MR Lange JM Miedema F Reiss P 《AIDS (London, England)》1999,13(2):177-184
OBJECTIVE: Unusual clinical inflammatory syndromes associated with underlying previously unrecognized opportunistic infections are increasingly being noted shortly after starting highly active antiretroviral therapy (HAART). This study examined the possible relationship between such unexpected disease manifestations and in vitro parameters of microbial antigen-specific immune reactivity in patients infected with HIV-1 who had a Mycobacterium avium intracellulare or Mycobacterium xenopi infection. DESIGN: In vitro T-cell proliferation experiments were performed after specific stimulation of a patient's peripheral blood mononuclear cells (PBMC) with M. avium and M. xenopi antigen and non-specific stimulation with phytohaemagglutinin (PHA). The results were compared with appropriate controls. PATIENTS: Five patients who presented with unusual clinical syndromes associated with M. avium or M. xenopi infection within weeks of experiencing large rises in CD4+ cell counts following the initiation of antiretroviral therapy. RESULTS: In all patients except one, mycobacteria-specific lymphoproliferative responses rose significantly following HAART; this was temporally associated with elevations in CD4+ cell counts and the occurrence of clinical disease. The patient with M. xenopi infection appeared to clear his infection subsequently without antimycobacterial therapy. In three of the four patients with M. avium infection, antimycobacterial treatment could be stopped without recurrence of infection. CONCLUSION: Our findings support the hypothesis that HAART may lead to clinically relevant inflammation as a result of restoration of specific immune reactivity against microbial pathogens that are subclinically present at the time treatment is initiated. Continuation of HAART may subsequently result in protective immunity and clearance of infection. 相似文献
9.
Wegdam-Blans MC Kampschreur LM Delsing CE Bleeker-Rovers CP Sprong T van Kasteren ME Notermans DW Renders NH Bijlmer HA Lestrade PJ Koopmans MP Nabuurs-Franssen MH Oosterheert JJ;Dutch Q fever Consensus Group 《The Journal of infection》2012,64(3):247-259
A review was performed to determine clinical aspects and diagnostic tools for chronic Q fever. We present a Dutch guideline based on literature and clinical experience with chronic Q fever patients in The Netherlands so far. In this guideline diagnosis is categorized as proven, possible or probable chronic infection based on serology, PCR, clinical symptoms, risk factors and diagnostic imaging. 相似文献
10.
Abraham C. J. Stork W-Ludo van der Pol Hessel Franssen Bart C. Jacobs Nicolette C. Notermans 《Journal of neurology》2014,261(7):1398-1404
The objective of this study was to investigate if the clinical and electrophysiological phenotype of patients with polyneuropathy associated with IgM monoclonal gammopathy (IgM-PNP) is related to the presence of antibodies against gangliosides or myelin-associated glycoprotein (MAG). We compared clinical and nerve conduction study (NCS) characteristics of 11 IgM-PNP patients with antibodies against asialo-GM1 or gangliosides (GM1, GD1a, GD1b, GM2 or GQ1b) to 11 consecutive IgM-PNP patients with anti-MAG neuropathy and to 9 IgM-PNP patients without antibodies against either MAG or gangliosides. Patients with anti-ganglioside antibodies could not be differentiated from those with anti-MAG antibodies based on clinical characteristics. However, within the group of anti-ganglioside antibody positive patients, antibodies against GD1b and GQ1b were associated with a purely sensory neuropathy (p = 0.002), while asymmetric weakness with symmetric sensory loss was associated with anti-asialo-GM1 antibodies. In conclusion, polyneuropathy associated with IgM monoclonal gammopathy and anti-ganglioside antibodies clinically resembles anti-MAG neuropathy. Pure sensory neuropathy and marked asymmetry may suggest the presence of anti-ganglioside rather than anti-MAG antibodies. 相似文献