全文获取类型
收费全文 | 25582篇 |
免费 | 1520篇 |
国内免费 | 399篇 |
学科分类
医药卫生 | 27501篇 |
出版年
2024年 | 21篇 |
2023年 | 185篇 |
2022年 | 245篇 |
2021年 | 592篇 |
2020年 | 432篇 |
2019年 | 607篇 |
2018年 | 914篇 |
2017年 | 694篇 |
2016年 | 817篇 |
2015年 | 895篇 |
2014年 | 1250篇 |
2013年 | 1496篇 |
2012年 | 1950篇 |
2011年 | 2163篇 |
2010年 | 1181篇 |
2009年 | 718篇 |
2008年 | 1361篇 |
2007年 | 1658篇 |
2006年 | 1607篇 |
2005年 | 1561篇 |
2004年 | 1537篇 |
2003年 | 1513篇 |
2002年 | 1227篇 |
2001年 | 577篇 |
2000年 | 622篇 |
1999年 | 435篇 |
1998年 | 179篇 |
1997年 | 110篇 |
1996年 | 69篇 |
1995年 | 79篇 |
1994年 | 49篇 |
1993年 | 42篇 |
1992年 | 64篇 |
1991年 | 48篇 |
1990年 | 56篇 |
1989年 | 31篇 |
1988年 | 33篇 |
1987年 | 39篇 |
1986年 | 25篇 |
1985年 | 34篇 |
1984年 | 20篇 |
1983年 | 27篇 |
1982年 | 28篇 |
1981年 | 35篇 |
1980年 | 22篇 |
1979年 | 27篇 |
1978年 | 24篇 |
1977年 | 19篇 |
1976年 | 15篇 |
1975年 | 20篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
Xiao-Long Tang Yan-Dong Miao Deng-Hai Mi 《World journal of gastrointestinal oncology》2022,14(1):366-368
The present letter to the editor is in response to the research “Outcomes of curative liver resection for hepatocellular carcinoma in patients with cirrhosis” by Elshaarawy et al in World J Gastroenterol 2021; 13(5): 424–439. The preoperative assessment of the liver reserve function in hepatocellular carcinoma (HCC) patients with cirrhosis is crucial, and there is no universal consensus on how to assess it. Based on a retrospective study, Elshaarawy et al investigated the impact of various classical clinical indicators on liver failure and the prognosis after hepatectomy in HCC patients with cirrhosis. We recommend that we should strive to explore new appraisal indicators, such as the indocyanine green retention rate at 15 min. 相似文献
2.
Qian Li Tian-Le Ma You-Qi Qiu Wen-Qiang Cui Teng Chen Wen-Wen Zhang Jing Wang Qi-Liang Mao-Ying Wen-Li Mi Yan-Qing Wang Yu-Xia Chu 《神经科学通报》2020,36(12):1484
Trigeminal neuralgia is a debilitating condition, and the pain easily spreads to other parts of the face. Here, we established a mouse model of partial transection of the infraorbital nerve (pT-ION) and found that the Connexin 36 (Cx36) inhibitor mefloquine caused greater alleviation of pT-ION-induced cold allodynia compared to the reduction of mechanical allodynia. Mefloquine reversed the pT-ION-induced upregulation of Cx36, glutamate receptor ionotropic kainate 2 (GluK2), transient receptor potential ankyrin 1 (TRPA1), and phosphorylated extracellular signal regulated kinase (p-ERK) in the trigeminal ganglion. Cold allodynia but not mechanical allodynia induced by pT-ION or by virus-mediated overexpression of Cx36 in the trigeminal ganglion was reversed by the GluK2 antagonist NS102, and knocking down Cx36 expression in Nav1.8-expressing nociceptors by injecting virus into the orofacial skin area of Nav1.8-Cre mice attenuated cold allodynia but not mechanical allodynia. In conclusion, we show that Cx36 contributes greatly to the development of orofacial pain hypersensitivity through GluK2, TRPA1, and p-ERK signaling.Electronic supplementary materialThe online version of this article (10.1007/s12264-020-00594-4) contains supplementary material, which is available to authorized users. 相似文献
3.
4.
5.
Agata Cieślik-Bielecka Paweł Reichert Rafał Skowroński Tomasz Bielecki 《Platelets》2019,30(6):728-736
The current literature suggests that the antibacterial effect of leukocyte- and platelet-rich plasma (L-PRP) is directly related to platelet and leukocyte concentrations. The aim of this study was twofold: first, to evaluate the antimicrobial effect of L-PRP against selected bacterial strains in vitro, and second, to correlate this effect with leukocyte and platelet content in the final concentration. Blood was collected from 20 healthy males, and L-PRP, acellular plasma (AP), and autologous thrombin were consecutively prepared. Flow cytometry analysis of the blood, L-PRP, and AP was performed. The L-PRP gel, liquid L-PRP, and thrombin samples were tested in vitro for their antibacterial properties against seven selected bacterial strains using the Kirby–Bauer disk-diffusion method. There was notable antimicrobial activity against selected bacterial strains. No statistically significant correlations between antimicrobial activities and the platelet concentration in L-PRP were observed. Statistically significant positive correlations between selected leukocyte subtypes and antimicrobial activity were noted. A negative correlation was found between elevated monocyte count and antimicrobial activity of L-PRP against one bacterial strain studied. L-PRP possesses antimicrobial activity and can be potentially useful in the fight against certain postoperative infections. The bactericidal effect of L-PRP is caused by leukocytes, and there exists a relationship among selected leukocyte subtypes and L-PRP antimicrobial activity. 相似文献
6.
Inflammation but not obesity or insulin resistance is associated with increased plasma fibroblast growth factor 23 concentration in the elderly 下载免费PDF全文
7.
8.
Konrad Matyja Anna Małachowska-Jutsz Anna K. Mazur Kazimierz Grabas 《Ecotoxicology (London, England)》2016,25(5):924-939
Dehydrogenase activity is frequently used to assess the general condition of microorganisms in soil and activated sludge. Many studies have investigated the inhibition of dehydrogenase activity by various compounds, including heavy metal ions. However, the time after which the measurements are carried out is often chosen arbitrarily. Thus, it can be difficult to estimate how the toxic effects of compounds vary during the reaction and when the maximum of the effect would be reached. Hence, the aim of this study was to create simple and useful mathematical model describing changes in dehydrogenase activity during exposure to substances that inactivate enzymes. Our model is based on the Lagergrens pseudo-first-order equation, the rate of chemical reactions, enzyme activity, and inactivation and was created to describe short-term changes in dehydrogenase activity. The main assumption of our model is that toxic substances cause irreversible inactivation of enzyme units. The model is able to predict the maximum direct toxic effect (MDTE) and the time to reach this maximum (TMDTE). In order to validate our model, we present two examples: inactivation of dehydrogenase in microorganisms in soil and activated sludge. The model was applied successfully for cadmium and copper ions. Our results indicate that the predicted MDTE and TMDTE are more appropriate than EC50 and IC50 for toxicity assessments, except for long exposure times. 相似文献
9.
10.
Lee Mi Jung Romero Sergio Velozo Craig A. Gruber-Baldini Ann L. Shulman Lisa M. 《Quality of life research》2019,28(6):1595-1603
Quality of Life Research - This study investigated the PROMIS Self-Efficacy Measure for Managing Chronic Conditions (PROMIS-SE) domain distributions and examined the factor structure of the... 相似文献