Morphological analysis of cementoenamel junction in premolars of Sri Lankans

Anatomical Science International - Cementoenamel junction is an anatomical landmark which indicates the meeting point of enamel of the crown and the cementum of the root. It is an important...     

An immunohistochemical study of the attachment mechanisms in different kinds of adhesive interfaces in teeth and alveolar bone of the rat

BACKGROUND AND OBJECTIVE: This study was designed to examine the histological and immunohistochemical nature of different kinds of adhesive interfaces in the rat molar region under identical experimental conditions and to discuss the structural and functional similarities between these adhesive interfaces. MATERIAL AND METHODS: Four kinds of adhesive interfaces - an initial attachment layer for principal fibers on the developing alveolar bone surface, a reattachment layer for principal fibers on resorbed alveolar bone surface, cement lines on the alveolar bone surface unrelated to the principal fibers, and the cemento-dentinal junction - were examined in 25-d-old male Wistar rats. Routine histological staining, immunohistochemical staining for bone sialoprotein and osteopontin, and digestion tests with trypsin were conducted. RESULTS: The adhesive interfaces showed very similar histological and immunohistochemical features: they were intensely hematoxylin-stainable, deficient in collagen fibrils, and rich in bone sialoprotein and osteopontin. After trypsin treatment the four adhesive interfaces had lost immunoreactivity to bone sialoprotein and osteopontin, and the two adjacent tissue parts held together finally separated at the adhesive interfaces. CONCLUSION: The above findings suggest that (i) the different types of adhesive interfaces in the rat molar region have a common structure in that they are filled with highly accumulated bone sialoprotein and osteopontin and deficient in collagen fibrils; (ii) accumulated bone sialoprotein and osteopontin are closely associated with the adhesion at the interfaces; and (iii) the adhesive interfaces have a similar developmental process.     

Immunohistochemical characterization of noncollagenous matrix molecules on the alveolar bone surface at the initial principal fiber attachment in rat molars

This study was designed to immunodetect proteoglycans (PGs) and the noncollagenous glycoproteins, bone sialoprotein (BSP) and osteopontin (OPN) on developing alveolar bone surface in rat molars by the indirect immunoperoxidase method, and to discuss the roles of these molecules at the initial principal fiber (PF) attachment. To characterize PGs, antibodies against five species of glycosaminoglycans (GAGs), chondroitin-4-sulfate (C4S), chondroitin-6-sulfate (C6S), unsulfated chondroitin (C0S), dermatan sulfate (DS), and keratan sulfate (KS) were used. Maxillary alveolar bone facing the distal root of the second molar was examined in 20- and 25-day-old male Wistar rats. Routine histological staining was also used. A hematoxylin-stained, fibril-poor layer always appeared on the alveolar bone surface just prior to the initial PF organization. This layer was strongly immunoreactive for C4S, C0S, OPN, and BSP, and weakly for C6S, but not for DS and KS. Then the initial PFs were attached to this layer. When new bone containing Sharpey's fibers covered this layer, it remained as a hematoxylin-stained, fibril-poor layer between Sharpey's fiber-containing and -lacking bone. The layer was consistently immunoreactive for OPN and BSP but had no immunoreactivity for GAGs. The results suggest that the accumulation of C4S-, C0S-, and C6S-carrying PGs, and of BSP and OPN is a primary event at the initial PF attachment, and is involved in the adhesion of PFs and mineralization of the initial attachment layer. The BSP and OPN act to maintain the interface integrity between Sharpey's fiber-containing and Sharpey's fiber-lacking alveolar bone after the PF attachment is established.