A Morphometric Study of the Lungs after Blast lnjury in Rats

A lot of research work has been carriod out on thc lungs after blast injury, but nomorphomctric study of the blast-injured lungs has ever been reported so far.The authors quantifiedthe morphological changes of the lungs with blast injury in rats with stereological methods.t wasfound that following the blast injury,the pulmonary capillary volume and the intcmal surface arca de-creased significantly,while the arithmetic and harmonic mean thickness of the air-blood barrier andthe volume of polymorphonuclear leucocytes and platelets in the capillaries incrcased markedly.Themorphometric oxygen diffusion capacity of the lungs was remarkably reduced mainly due to the de-crease of the conductance of the air-blood barrier and erythrocytcs.In addition,the changes of theoxygen diffussion capacity of the lungs were closely related to the partial pressure of arteria/blood oxy-gen.These facts imply that the damage to the pulmonary capillaries was one of the main causes ofalveolar bleeding and early respiratory insufficiency.It is possible that a mathematic relationshipcould be established between the intensity of the blast impact and the consequent morphologicalchanges of the injured lungs.     

三种止血带对骨骼肌组织结构的效应

为了探讨止血带使用的安全时限，我们用新研制的局部充气止血带对犬进行了实验，并用橡皮管止血带和环绕充气止血带作为对照，比较三种止血带对肢体的影响。     

病变血细胞膜对电磁波特异反应的微观表现

我们使用正电子湮没寿命谱(PALS)和电镜技术等研究了病变血细胞膜对激光和γ射线特异反应的某些微观表现,例如相变、电荷分布及流变学特性等。某些结果颇有意义:肺癌病人(7例)与正常人(10例)作对照的血样用PALS分析,谱的拟合三种组分有显著性差别,它表明病变血细胞膜的电子密度比正常人的明显偏高。柯兴氏综合症病人的血样经He—Ne激光(约50mW)照射后做电镜观察:某些白细胞膜出现分凝现象;此外,还发现高电子密度的环状包绕物包裹在细胞外周,而正常人血细胞膜作为     

冲击伤时肺泡上皮细胞和毛细血管内皮细胞膜内微粒分布的变化

冲击伤时肺泡上皮细胞和毛细血管内皮细胞膜内微粒分布的变化朱国标，甘韶雨，张克俊，王正国，王声文业已证实，冲击伤时大鼠肺泡上皮细胞和毛细血管内皮细胞结构和功能均有显著变化［１，２］，但对细胞膜结构的变化所知甚少［３］。本研究旨在采用冷冻蚀刻技术定量分析...     

实验性急性肾衰时血液流变学的研究

家兔甘油性急性肾衰时全血和血浆粘度,纤维蛋白原和血小板聚集率明显增加,电镜检查也见血栓形成和红细胞聚集。本文就上述改变的原因和意义进行了讨论。     

异种脱钙骨基质、骨基质胶加释放系统移植的组织学和电镜观察

XDBM XBMG用小公牛长管状骨制备;释放系统由 CaSO_1、Na_2HPO_1、KH_2PO:(DSA）、Ca-Cl_2、Na_2HPO_4、KH_2PO_1(DSB)组成。Wistar大鼠24只分四组:(1)XDBM加DSA;(2)XDBM加DSB;(3)XBMG加DSA;(4)XBMG加DSB;不设对照。将四种移植物分别植入大鼠股四头肌肌穴。定期取材制片,TEM—100SX透射电镜观察。结果:2天巨噬细胞、淋巴细胞密集,细胞器发达,微绒毛状突起;4天间充质细胞贴附在XDBM、XBMG表面,细胞器发达伪足伸入移植物缝隙;8天成软骨细胞活跃,软骨细胞少;14天软骨细胞中部分细胞的细胞器固缩、溶解;22天网状骨小梁,骨细胞突起部分相互连接;34天新生骨渐成熟,骨髓细胞,个别哈氏管出现;几种生骨细胞和间充质细胞常在一个视野或网眼内,相贴很近,始终未发现破骨细胞。实验证明:XDBM、XBMG加DS诱导成骨的细胞来源是间充质细胞,直到34天仍见巨噬细胞存在尚未转化为破骨细胞。     

PVC袋装注射液空袋处理工艺的研究

ＰＶＣ袋装注射液空袋处理工艺的研究李新生，王声文，孙贯一，代远明（第三军医大学第三附属医院重庆市６３００４２）随着塑料工艺的发展，自６０年代起国内外开始出现了使用塑料容器灌装输液和输血，目前常用聚氯乙烯（ＰＶＣ）制成输液（血）用容器。我们所根据需要研...     

大鼠冲击伤时肺毛细血管内皮细胞超微结构的变化

本实验应用超薄切片、硝酸镧示踪、微血管铸型及冷冻蚀刻等电镜技术,观察了大鼠冲击伤时肺毛细血管内皮细胞超微结构的变化。结果显示,肺毛细血管内皮细胞及其细胞紧密连接明显破坏,其对硝酸镧及甲基丙烯酸甲酯的通透性显著增加。     

Stereological study of quantitative relationships between morphological and functional changes of the lungs in early phase of blast injury in rats

The morphological and functional changes of the lungs in the early phase of blast injurywere analyzed and related quantitatively in rats with microcomputer-assisted morphometry.It wasfound that(1)significant or partial correlation exists between stereological and functional indices,(2)changes of the endothelium and epithelium rather than the interstitium contribute to the increase ofharmonic and arithmetic thickness(T_(ht),T_(at))of the air-blood barrier,and(3)the regression equa-tions are as follows:(a)PaO_2=3.56+1.62DL(O_2),r=0.678,P=0.032:(b)PaO_2=2.99+3.49DL(O_2)-0.0005S_A+0.003S_c-38.2V_c-0.257T_(ap)-0.028Dt(O_2),r_y=0.9999,P=0.008;(c)PaCO_2=7.69+0.0001S_A-4.35T_(at)+19.7T_(ht)-46.2T_(hp)-4.4T_(aen)-5.87T_(aep),r_y=0.9999,P=0.014;(d)pH=3.068+0.0005S_A-1.79T_(at)+12.2T_(ht)-50.8T_(hp)-2.52T_(aint)+0.69DL(O_2),r_y=0.9999,P=0.10.The significance and application of stereology in theestablishment of the quantitative relationship between morphological and functional indices are dis-cussed.Mathematical models to relate morphometric parameters to functional changes are possible.     

Morphological Changes of the Tight Junction of Pulmonary Epithelium and Capillary Endothelium after Blast Injury in Rats

The morphological changes of the tight junction of the alveolar epithelium andthe capillary endothelium of the lungs after blast injury in rats were studied with freeze-etching elec-tron microscopic technique.The results were as follows:1.The number of the discontiuities,discontinuities per μm,irregularity,and free-ending