Effects of basic fibroblast growth factor on biological characteristics of osteoblasts

Objective: To elucidate the effects of exogenous basic fibroblast growth factor ( bFGF ) on biological characteristics of rat osteoblasts cultured in vitro. Methods: The osteoblasts isolated from a Sprague-Dawley rat and cultured in vitro were treated with different concentrations of bFGF ( 5-50 ng/ml) respectively. At 24 hours after treatment, the proliferating cell nuclear antigen was measured with immunocytochemistry, alkaline phosphatase (ALP) activity was determined and the expression of transforming growth factor beta 1 ( TGF-β1 )was detected to observe the effects of bFGF on growth and differentiation of osteoblasts. Resu/ts: bFGF ( 5-50 ng/ml ) could obviously promote the growth of osteoblasts. The intracellular expression of TGF-β1 mRNA increased significantly, but the intracellular ALP content decreased. Conclusions: bFGF can obviously stimulate the proliferation of osteoblasts and promote the synthesis of TGF-β1, but cannot promote the differentiation of osteoblasts.     

多发性骨折后全身炎症反应综合征的监控

目的　分析多发性骨折后全身炎症反应综合征 (SIRS)的监测因素。方法　将 87例多发性骨折患者分为SIRS组、非SIRS组和死亡组 ,比较各组血清肿瘤坏死因子 (TNF -α)、粒细胞集落刺激因子 (G -CSF)、补体C3 、免疫球蛋白 (IgG)等的变化。同时观察失血量分级与SIRS的关系。结果　SIRS组G -CSF和IgG水平明显低于非SIRS组 ,TNF -α水平明显高于非SIRS组 (P <0 .0 5 ) ;死亡组G -CSF、补体C3 及IgG水平较SIRS组进一步降低 ,TNF -α水平进一步升高 (P <0 .0 5 )。随失血量增大 ,SIRS发生率、病死率升高 (P <0 .0 5 )。结论　G -CSF ,IgG ,TNF -α水平及失血量可作为SIRS的监控指标。     

Abmessung und Analyse der Bezugsflche von Acetabulum-Prothese-Transplantationin unterschiedlichen Krperlagen

Bei der Permutationsoperation des Pfannenge-lenks ist die Genauigkeit des Transplantation-swinkels der H櫣ftpfannen-Prothese ein wichtigerFaktor bez櫣glich der Auswirkung der Operation.Schlechte Positionierung der H櫣ftpfannenprothesekann zu Komplikationen wie Luxation nach Opera-tion,starke Verschleiss der Prothese,Lockerungder Prothese usw.f櫣hren[1,2].Bei der Transplan-tation der H櫣ftpfannenprothese wird meistens dieFlache zwischen Ant.Sup.Iliac spine auf beidenSeiten und Pubic t…     

Ultrasonographic Evaluation of Osteosarcomas

The ultrasonographic appearances of osteosarcomas and the roles of ultrasonography in the diagnosis and surgical staging of osteosarcomas were investigated. A comparative study was performed on 45 cases of osteosarcomas by ultrasonography and radiography. Bony changes, periosteal reaction and soft tissue mass were evaluated for each lesion. The results showed that ultrasonography revealed a solid mass around bone in 42 patients, bone destruction in 24 patients and periosteal reaction in 16 patients. Plain radiographs showed bony changes in 44 patients and no bony change in remaining one patient, shadowing of soft tissue swelling in 30 patients, and pulmonary metastases in 3 patients. Surgical biopsy and pathological examination confirmed osteosarcoma in all 45 patients. Soft tissue mass was confirmed in 42 patients surgically. The diagnostic accuracy of soft tissue masses by ultrasonography and radiography was 100 % (42/42) and 71.4 % (30/42), respectively. The positive rate of ultrasonography and radiography in displaying bony changes was 53.3 % (24/45) and 97.8 % (44/45), respectively. In conclusion, in the detection of soft tissue mass of osteosarcoma, ultrasonography is superior to radiography, and in displaying bony changes of osteosarcomas, radiography is superior to ultrasonography. So it may come to a conclusion that plain radiography combined with ultrasonography can completely display the bony and soft tissue lesion of osteosarcomas.     

骨髓细胞检查在贫血病因诊断中的临床价值

目的研究分析骨髓细胞检查在诊断贫血病因诊断方面的临床价值。方法选择2011年3月~2014年3月在我院收治的200例贫血患者,对其行骨髓的细胞学检查,并对检查结果进行详细分析以判断其病因。结果有179例(89.5%)患者被诊断出分别患有12种以上的疾病,但仍有21例(10.5%)患者的疾病原因不能被诊断出。结论目前临床的骨髓细胞学检查为贫血患者的病因诊断提供了重要且可靠性的依据,但仍有一定的局限性存在。     

艾滋病合并肺结核35例CT征象分析

目的 分析艾滋病合并肺结核35例患者的临床资料及CT征象分析,总结其CT影像特点,提高对艾滋病合并肺结核的影像诊断水平.方法 收集35例艾滋病合并肺结核患者的临床资料及CT征象,分析和探讨艾滋病合并肺结核的CT特点.结果 艾滋病合并肺结核患者,其临床症状重,但胸部影像表现多不典型:(1)双肺不典型血播多见,表现为双肺弥漫粟粒结节影并广泛间质增厚,部分融合成小片状.(2)1～2个肺段的片团状实变多见,出现干酪坏死少见.(3)双肺毛玻璃影多见.(4)合并多浆膜腔积液(双侧胸腔积液及心包积液)多见.(5)空洞少见.结论 艾滋病合并肺结核的临床表现不典型,CT表现有一定特点,早期临床医生应该高度重视,提高其诊断率.     

距骨颈骨折的手术治疗及疗效分析(附31例报告)

目的探讨距骨颈骨折的手术治疗方案及疗效。方法随访43例距骨颈骨折手术治疗患者,行闭合或切开复位,以皮质骨螺钉、松质骨螺钉、空心螺纹钉或可吸收螺钉进行固定。术后均石膏固定,早期功能康复锻炼。结果平均随访时间24个月以上。术后按照Howkins疗效标准进行疗效评价,其中优13例,良9例,可6例,差3例,优良率70.3%。术后2例发生局部皮肤坏死。8例发生距骨缺血性坏死,7例踝关节创伤性关节炎,距下关节创伤性关节炎14例,内翻畸形愈合2例。结论选择恰当的复位、手术时机、手术方法,尽量保护软组织,早期进行功能康复锻炼,可获得较好的临床疗效。     

青少年Pilon骨折11例报告

胫骨Piln骨折是胫骨远端关节内骨折，最早由Destot于1911年提出，约占胫骨骨折的79％，虽然其发病率较低，但治疗结果却不容乐观。目前关于胫骨Pilon骨折的报道大多为成人的，关于青少年Pilon骨折的报告则较少。我院自1997年4月至2004年8月先后收治青少年Pilon骨折11例，现总结报告如下。     

单侧椎板开窗髓核摘除术治疗中央型椎间盘突出症

[目的]探讨单侧椎板间开窗髓核摘除术治疗中央型腰椎间盘突出症适应证及疗效. [方法]对自2004年1月～2006年12月收治66例确诊中央型腰椎闻盘突出症患者行单侧椎板问开窗髓核摘除术. [结果]全部病例得到3～36个月随访(平均17个月).疗效按照NaKai评定标准,其中优48例,良13例,可4例,差1例.优良率92.4%.疗效差1例分析原因可能因为术中过度牵拉神经根所致,予以卧床休患2周,并加用激素、神经营养药物等,2周后缓解.本组病例无意外损伤及感染等病例发生. [结论]单侧椎板间开窗髓核摘除术是治疗中央型腰椎间盘突出症有效方法,临床效果满意.