Visualization of mitochondria with green fluorescent protein in cultured fibroblasts from patients with mitochondrial diseases

cDNAs for green fluorescent protein (GFP) and for a GFP fusion protein containing the presequence of human ornithine transcarbamylase (pOTC-GFP) were transfected into cultured human fibroblasts. GFP cDNA gave diffuse fluorescence throughout the cytoplasm and the nucleus, whereas pOTC-GFP cDNA gave mitochondria-associated fluorescence. Fluorescent mitochondrial structures could be classified into five patterns: thread-like mitochondria, fine thread-like ones, rod-like ones, granular ones, and granular ones with weak cytosolic fluorescence. pOTC-GFP mutants resulted in a loss of mitochondrial fluorescence and an appearance of weak fluorescence throughout the cytoplasm. pOTC-GFP cDNA was transfected into fibroblasts from patients with various mitochondrial diseases. Higher ratios of fibroblasts with granular mitochondria and those with fine thread-like ones were observed in a patient with Reye's syndrome and a patient with Kearns-Sayre syndrome. Weak cytosolic fluorescence was sometimes observed in fibroblasts from these patients. This method will be useful to analyze mitochondrial structural alterations and disorders of mitochondrial protein import.     

A feasibility study of a direct-mapping parallel processing method to solve linear equations in load-flow calculation

With the growing size and complexity of power systems, system analysis—such as transients calculation—takes much time. Hence, fast calculation methods are required. Although parallel processing is a hopeful method, there have been difficulties in the parallel solution of linear equations which appear in power-flow calculations by the Newton-Raphson method. This paper aims at the fast calculation of the power-flow problem by means of parallel processing. In order to improve the suitability to the parallel solution of the differential equation in transients calculation, we assume the use of a direct-mapping parallel processing machine to map directly the network of a power system onto a network of processors. Under this assumption, we propose a new parallel-processing-oriented method in which the linear equation is solved by linear iterations between nodes with Aitken acceleration. We simulate the method on three model power systems and compare this Parallel Iterative Method (PIN) with a Parallel Direct Method (PDM) which uses the banded matrix according to the number of operations required. As a result, we can expect that PIM may solve linear equations faster than PDM with m processors, although the PIM might be inferior to the PDM with m × m processors, where m denotes the half-band width of the banded matrix.     

Generation of adaptive gait patterns for quadruped robot with CPG network including motor dynamic model

This paper describes the generation of adaptive gait patterns using new Central Pattern Generators (CPGs) including motor dynamic models for a quadruped robot under various environments. The CPGs act as the flexible oscillators of the joints and adjust joint angles to required values. The CPGs are interconnected with each other and sets of their coupling parameters are adjusted by a genetic algorithm so that the quadruped robot can realize stable and adequate gait patterns. Generation of gait patterns results in the formation of the CPG networks suitable for the formation of not only a straight walking pattern but also of rotating gait patterns. Experimental results demonstrate that the proposed CPG networks are effective for the automatic adjustment of the adaptive gait patterns for the tested quadruped robot under various environments. Furthermore, the target tracking control based on image processing is achieved by combining the general gait patterns. © 2006 Wiley Periodicals, Inc. Electr Eng Jpn, 155(1): 35–43, 2006; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20225     

Electrochemical formation of AlN in molten LiCl-KCl-Li3N systems

Electrochemical formation of aluminum nitride was investigated in molten LiCl-KCl-Li₃N systems at 723 K. When Al was anodically polarized at 1.0 V (versus Li⁺/Li), oxidation of nitride ions proceeded to form adsorbed nitrogen atoms, which reacted with the surface to form AlN film. The obtained nitrided film had a thickness of sub-micron order. The obtained nitrided layer consisted of two regions; the outer layer involving AlN and aluminum oxynitride and the inner layer involving metallic Al and AlN. When Al electrode was anodically polarized at 2.0 V, anodic dissolution of Al electrode occurred to give aluminum ions, which reacted with nitride ions in the melt to produce AlN particles (1-5 μm of diameter) of wurtzite structure.     

Two‐way multi‐view 2‐D/3‐D display combining LC lens and HVxDP panel using novel pixel arrangement

We have developed a two‐way multi‐view 2‐D/3‐D display combining a liquid crystal lens and horizontally and vertically x times‐density pixels (HVxDP) arrangement. The two‐way multi‐view display features the same display resolution in 2‐D and 3‐D modes and a quite small color moiré for landscape and portrait, respectively, when using the HVxDP arrangement. In this paper, we realized suitable 3‐D properties for achieving a good balance between 3‐D moiré and 3‐D crosstalk for landscape and portrait by a two‐way liquid crystal lens with two kinds of focal lengths for the edge part and the center part of the lens.     

Experimental measurement on tangential momentum accommodation coefficient in a single microtube

The tangential momentum accommodation coefficient (TMAC) was investigated experimentally from the mass flow rate through a single microtube under the slip flow and the early part of the transition regime. The measurements were carried out by the constant-volume method under the mean Knudsen number smaller than 0.3, which is based on the mean pressure of the inlet and the outlet of the microtube, to apply the second-order slip boundary condition. To measure TMACs on various materials, quite large microtube was employed, which require the reduction in leakage. TMAC was obtained from the slip coefficient determined by the relation of the mass flow rate to the mean Knudsen number. The obtained mass flow rate was well explained by the theoretical equation. TMACs of deactivated-fused silica with argon, nitrogen, and oxygen were measured, showing the tangential momentum was not accommodated completely to the surface, and the values showed good agreement with previous studies. From the comparison between microtubes with different inner diameter, it is showed that TMAC is determined mainly by gas species and surface material.     

Traceable P2P record exchange: a database-oriented approach

In recent years, peer-to-peer (P2P) technologies are used for flexible and scalable information exchange in the Internet, but there exist problems to be solved for reliable information exchange. It is important to trace how data circulates between peers and how data modifications are performed during the circulation before reaching the destination for enhancing the reliability of exchanged information. However, such lineage tracing is not easy in current P2P networks, since data replications and modifications are performed independently by autonomous peers—this creates a lack of reliability among the records exchanged. In this paper, we propose a framework for traceable record exchange in a P2P network. By managing historical information in distributed peers, we make the modification and exchange histories of records traceable. One of the features of our work is that the database technologies are utilized for realizing the framework. Histories are maintained in a relational database in each peer, and tracing queries are written in the datalog query language and executed in a P2P network by cooperating peers. This paper describes the concept of the framework and overviews the approach to query processing.     

Avoiding Routing Loops on the Internet

Suppose that some particular link in the Internet is currently congested. A natural solution is to try to make packets bypass that link. This can be done by increasing the cost of that link intentionally, say from a ₁ to a ₂, since the Internet uses shortest-path routing. Unfortunately, however, this often causes temporary loops for packet traveling, called routing loops. In this paper we show that routing loops can be avoided by increasing the cost of the link not directly from a ₁ to a ₂ but through an intermediate value, a ₃, i.e., from a ₁ to a ₃ and then to a ₂. We may need several intermediate values. We show that in this case the greedy strategy, namely, raising the cost as much as possible in each step, is optimal.     

The Detection of Immunity against WT1 and SMAD4P130L of EpCAM+ Cancer Cells in Malignant Pleural Effusion

Malignant pleural effusion (MPE) provides a liquid tumor microenvironment model that includes cancer cells and immune cells. However, the characteristics of tumor antigen-specific CD8⁺ T cells have not been investigated in detail. Here, we analyzed MPE samples taken from a patient with pancreatic cancer who received a dendritic cell vaccine targeting Wilms’ Tumor 1 (WT1) antigen over the disease course (two points at MPE^1st and 2^nd, two months after MPE1^st). Epithelial cell adhesion molecule (EpCAM)⁺ cancer cells (PD-L1⁻ or T cell immunoglobulin mucin-3, TIM-3⁻), both PD-1 or TIM-3 positive CD8⁺ T cells, and CD14⁺CD68⁺CD163⁺TIM-3⁺ macrophages increased from the MPE^1st to MPE^2nd. The ratio of WT1-specific cytotoxic lymphocytes (WT1-CTLs) to MPE CD8⁺ T cells and IFN-γ secretion of WT1-CTLs were reduced with disease progression. Coincidentally, the fraction of central memory T (T_CM) of WT1-CTLs was decreased. On the other hand, CD8⁺ T cells in response to SMAD4^P130L, which is homogeneously expressed in EpCAM⁺ cancer cells, were detected using in vitro expansion with the HLA-A*11:01 restrictive SVCVNLYH neoantigen. Furthermore, the CD8⁺ T cell response to SMAD4^P130L was diminished following remarkably decreased numbers of CD8⁺ T_CM in MPE samples. In conclusion, CD8⁺ T cells responding to WT1 or SMAD4^P130L neoantigen expressed in EpCAM⁺ pancreatic cancer cells were detected in MPE. A tumor antigen-specific immune response would provide novel insight into the MPE microenvironment.