Application of thermal ultrasound-assisted liquid–liquid micro-extraction coupled with HPLC-UV for rapid determination of synthetic phenolic antioxidants in edible oils

A simple, fast, and reliable liquid–liquid micro-extraction (LLME) method assisted by thermal ultrasound approach was developed for simultaneous determination of synthetic phenolic antioxidants (SPAs) in edible oils by high-performance liquid chromatography equipped with ultraviolet detector (HPLC-UV). The synthetic antioxidants were propyl gallate (PG), butylated hydroxyanisole (BHA), tert-butylhydroquinone (TBHQ), and butylated hydroxyltoluene (BHT). The best extraction conditions were observed were methanol/acetonitrile (1:1, v/v) as the solvent, ultrasound at 4 min, and a temperature of 40°C. The linearity of the calibration curves for the optimum conditions were R² > 0.989 for all of the SPAs in a range from 1–200 μg ml⁻¹. Relative standard deviation (RSD %) for five analysis was in range of 2.83% to 4.21%. Limit of detection (LOD) and limit of quantification (LOQ) were obtained in range of 0.012–0.06 and 0.04–0.2 μg g⁻¹, respectively. With regard to recovery, a range of 91%–116% was calculated for the spiked edible oils.     

Relay-Assisted Wireless Energy Transfer for Efficient Spectrum Sharing in Harsh Environments

International Journal of Wireless Information Networks - With exponential growth in the number of wireless devices and limited available spectrum, the problem of spectrum sharing remains in...     

Multifunctional Gadolinium‐Doped Mesoporous TiO2 Nanobeads: Photoluminescence,Enhanced Spin Relaxation,and Reactive Oxygen Species Photogeneration,Beneficial for Cancer Diagnosis and Treatment

Materials with controllable multifunctional abilities for optical imaging (OI) and magnetic resonant imaging (MRI) that also can be used in photodynamic therapy are very interesting for future applications. Mesoporous TiO₂ sub‐micrometer particles are doped with gadolinium to improve photoluminescence functionality and spin relaxation for MRI, with the added benefit of enhanced generation of reactive oxygen species (ROS). The Gd‐doped TiO₂ exhibits red emission at 637 nm that is beneficial for OI and significantly improves MRI relaxation times, with a beneficial decrease in spin–lattice and spin–spin relaxation times. Density functional theory calculations show that Gd³⁺ ions introduce impurity energy levels inside the bandgap of anatase TiO₂, and also create dipoles that are beneficial for charge separation and decreased electron–hole recombination in the doped lattice. The Gd‐doped TiO₂ nanobeads (NBs) show enhanced ability for ROS monitored via ^?OH radical photogeneration, in comparison with undoped TiO₂ nanobeads and TiO₂ P25, for Gd‐doping up to 10%. Cellular internalization and biocompatibility of TiO₂@x Gd NBs are tested in vitro on MG‐63 human osteosarcoma cells, showing full biocompatibility. After photoactivation of the particles, anticancer trace by means of ROS photogeneration is observed just after 3 min irradiation.     

Microencapsulation of Nigella sativa seeds oil containing thymoquinone by spray‐drying for functional yogurt production

The present work reports on the microencapsulation of Nigella sativa seeds oil containing thymoquinone (TQ) by spray‐drying, using modified starch (MS) and maltodextrin (MD) mixture as wall materials aimed at producing functional yogurt. First, the impact of different ratios of MS/MD on microencapsulation efficiency (ME) and TQ retention was investigated. The highest ME (90.10%) was found in microcapsules prepared from emulsion with 80/20 ratio of MS/MD; however, the microcapsules prepared with 50/50 ratio was selected for considering TQ stability under storage conditions and functional yogurt production due to an acceptable ME (89.48%) and better TQ retention (61.12%). The results showed that the microcapsules stored at refrigerator temperature had the highest content of TQ after 4 weeks. Moreover, the results of chemical and sensory analysis suggest that N. sativa seeds oil microcapsules can be used for producing functional yogurt due to high stability of TQ and proper chemical and sensory properties.     

Sweet lemon mechanical damage detection using image processing technique and UV radiation

Non-destructive measurement of qualitative parameters of agricultural produce is quite beneficial in the postharvest operations. The consequential effect of mechanical damage in citrus fruits is rarely visible in their appearance compared to other commodities. The purpose of this study was to propose a fast, non-destructive method for sweet lemon mechanical damage detection using image processing technique and UV radiation. For this purpose, 135 sweet lemons were tested based on a completely randomized factorial design. In order to examine mechanical damage, the independent variables included drop height, fruit diameter and tempering period (holding time at room temperature after treatment). Fruits were dropped from heights of 2, 2.5 and 3 m onto the ground. Then images were captured under UV light having a wavelength of 365 nm, 1, 3 and 6 days after treatment. The images were sent to a PC and analyzed using MATLAB software. “Green Spot Index” or GSI was defined to show the extent of mechanical damage. Results of the analysis of variance showed that the percentage of green spots on fruit skin is significant at the 1% level considering the main and double interaction effects of drop height and fruit diameter. Green spot index significantly increases with the level of mechanical damage. Accuracy of the developed method in differentiating the bruised and undamaged fruits was found to be 100%.     

Tamoxifen-loaded solid lipid nanoparticles-induced apoptosis in breast cancer cell lines

An in vitro study was conducted to determine the apoptosis induced by tamoxifen (TAM) and TAM-loaded solid lipid nanoparticles (SLNs) in breast cancer cell lines, MCF-7 and MDA-MB231 cells. The effect of free drug and drug-loaded SLN on the cell lines was characterised by cell morphology and cell cycle distribution using phase contrast microscopy, nuclear morphology and flow cytometry, respectively. The results showed that TAM-loaded SLNs have an equally efficient cytotoxic activity against MCF-7 and MDA-MB231 cells, compared to free TAM, and the half maximal inhibitory concentration (IC₅₀) of TAM-loaded SLNs was generally lower than that of free TAM. In the presence of TAM and TAM-loaded SLN, the viability of the both cells diminishes and the cancer cells lose their normal morphological characteristics, detaches, aggregates and later develops apoptotic bodies. Flow cytometry analysis showed that TAM-loaded SLN like the free TAM caused a dose- and time-dependent apoptosis without cell cycle arrest of human breast cancer cells. Therefore, TAM-loaded SLN has great potential in human medicine for the treatment of breast cancers.