Genetic and Epigenomic Modifiers of Diabetic Neuropathy

Diabetic neuropathy (DN), the most common chronic and progressive complication of diabetes mellitus (DM), strongly affects patients’ quality of life. DN could be present as peripheral, autonomous or, clinically also relevant, uremic neuropathy. The etiopathogenesis of DN is multifactorial, and genetic components play a role both in its occurrence and clinical course. A number of gene polymorphisms in candidate genes have been assessed as susceptibility factors for DN, and most of them are linked to mechanisms such as reactive oxygen species production, neurovascular impairments and modified protein glycosylation, as well as immunomodulation and inflammation. Different epigenomic mechanisms such as DNA methylation, histone modifications and non-coding RNA action have been studied in DN, which also underline the importance of “metabolic memory” in DN appearance and progression. In this review, we summarize most of the relevant data in the field of genetics and epigenomics of DN, hoping they will become significant for diagnosis, therapy and prevention of DN.     

Flow of granules through cylindrical hopper

The gravity flow characteristics of pharmaceutically important granules were studied in a cylindrical hopper with attachable orifice disk at the bottom. Results indicate that materials property and orifice diameter play key role in determining mass flow rate while keeping environmental condition (temperature) at fixed range. A new dimensionally analyzed equation was developed to predict gravity flow of pharmaceutically important granules. The developed correlation agreed well with the experimental data. Calcium gluconate and lactose were used for preparing granules. Generally, glidant/lubricant materials are added to pharmaceutical granules during tablet manufacturing process, in order to improve physical property of granular material. Hence, the effect of glidant/lubricant on mass flow of granules through hopper was also studied. Magnesium stearate (1.5%) was added as glidant before granulation.     

Fast attack detection using correlation and summarizing of security alerts in grid computing networks

Due to the extensive growth of grid computing networks, security is becoming a challenge. Usual solutions are not enough to prevent sophisticated attacks fabricated by multiple users especially when the number of nodes connected to the network is changing over the time. Attackers can use multiple nodes to launch DDoS attacks which generate a large amount of security alerts. On the one hand, this large number of security alerts degrades the overall performance of the network and creates instability in the operation of the security management solutions. On the other hand, they can help in camouflaging other real attacks. To address these issues, a?correlation mechanism is proposed which reduces the security alerts and continue detecting attacks in grid computing networks. To obtain the more accurate results, a?major portion of the experiments are performed by launching DDoS and Brute Force (BF) attacks in real grid environment, i.e., the Grid??5000 (G5K) network.     

Overview of the potent cyanobacterial neurotoxin β-methylamino-L-alanine (BMAA) and its analytical determination

Blue-green algae are responsible for the production of different types of toxins which can be neurotoxic, hepatotoxic, cytotoxic and dermatotoxic and that can affect both aquatic and terrestrial life. Since its discovery the neurotoxin β-methylamino-L-alanine (BMAA) has been a cause for concern, being associated with the neurodegenerative disease amyotrophic lateral sclerosis/Parkinsonism–dementia complex (ALS/PDC). The initial focus was on Guam where it was observed that a high number of people were affected by the ALS/PDC complex. Subsequently, researchers were surprised to find levels of BMAA in post mortem brains from Canadian patients who also suffered from ALS/PDC. Recent research demonstrates that BMAA has been found at different levels in the aquatic food web in the brackish waters of the Baltic Sea. There is emerging evidence to suggest that sand-borne algae from Qatar can also contain BMAA. Furthermore, there is now concern because BMAA has been found not only in warmer regions of the world but also in temperate regions like Europe. The aim of this review is to focus on the methods of extraction and analysis of the neurotoxic non-protein amino acid BMAA. We also consider the neurotoxicity, aetiology, and diverse sources and routes of exposure to BMAA. In recent years, different methods have been developed for the analysis of BMAA. Some of these use HPLC-FD, UPLC-UV, UPLC-MS and LC-MS/MS using samples that have been derivatised or underivatised. To date the LC-MS/MS approach is the most widely used analytical technique as it is the most selective and sensitive method for BMAA determination.     

Understanding the influence of demographic and psychological variables on retirement planning.

The authors examined the degree to which workers were engaging in financial, health, interpersonal/leisure, and work planning for retirement, exploring whether demographic and psychological variables inhibited or promoted planning in each of these domains. Planning in each domain was influenced by a unique set of variables. Goals emerged as a consistent and positive predictor of planning. Gender accounted for health and interpersonal/leisure planning, while work planning behavior was negatively predicted by income. Time perspective also helped to clarify the amount of retirement planning undertaken in the financial and interpersonal/leisure domains. Practical implications for designing retirement interventions are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Tuneable electrical properties of hafnium aluminate gate dielectrics deposited by metal organic chemical vapour deposition

Hafnium aluminate (HfAlO) high-k films deposited by Metal Organic Chemical Vapour Deposition (MOCVD) with various Al concentrations were investigated. The results of electrical measurements show the feasibility of adjusting the relative dielectric constant of the layers in a wide range (9–16), when the aluminium concentration varies between 4% and 38%. The minimum leakage current occurs for Al concentrations up to 9%. The thinner films show Poole–Frenkel-like conduction at low field and Fowler–Nordheim-like conduction at moderate/high field, even at higher concentrations of Al into the film, while thicker films show a higher hysteresis due to an increased number of slow trapping centres in the film.     

Longitudinal trends in river functioning: Patterns of nutrient and carbon processing in three Australian rivers

Understanding longitudinal trends in the processing of carbon in rivers represents a much conceptualised, but infrequently tested, issue in aquatic ecology. In this study, we conducted concurrent longitudinal examinations of three very different rivers in eastern Australia to determine whether general principles in river functioning exist across broad geographic and hydrologic scales. Specifically, we examined trends in ambient basic water chemistry, nutrient concentrations, dissolved organic carbon (DOC), extracellular enzymes and food web structure and functioning and conducted bioassays to examine the degree to which DOC and nutrients limit heterotrophic bacterial respiration. These parameters revealed striking similarities across all sites. For metazoan communities, stable isotope analysis showed that algal carbon was the dominant basal resource utilised by consumers in all three rivers, suggesting that in‐stream primary producers strongly underpin trophic pathways regardless of the position within a catchment or catchment condition. Analyses of extracellular enzymes revealed that microbial communities are actively utilising DOC at all sites. In fact, heterotrophic microbial respiration was strongly limited by DOC at all sites, with nutrient additions resulting in only relatively minor increases in respiration. Ultimately, this study demonstrates that DOC and algal carbon are critically important drivers of ecosystem processes in Australian riverine ecosystems. Furthermore, across all of our sites and rivers, ambient nutrient concentrations did not influence carbon processing. The consistent longitudinal trends in river function identified in this study provide useful insights for catchment managers and modellers with respect to identifiying key principles that underpin ecosystem functioning in Australian rivers. Copyright © 2009 John Wiley & Sons, Ltd.