Damage localization for beams based on the wavelet correlation operator

The continuous wavelet transform (CWT) is one of the crucial damage identification tools in the vibration-based damage assessment. Because of the vanishing moment property, the CWT method is capable of featuring damage singularity in the higher scales, and separating the global trends and noise progressively. In the classical investigations about this issue, the localization property of the CWT is usually deemed as the most critical point. The abundant information provided by the scale-domain information and the corresponding effectiveness are, however, neglected to some extent. Ultimately, this neglect restricts the sufficient application of the CWT method in damage localization, especially in noisy conditions. In order to address this problem, the wavelet correlation operator is introduced into the CWT damage detection method as a post-processing. By means of the correlations among different scales, the proposed operator suppresses noise, cancels global trends, and intensifies the damage features for various mode shapes. The proposed method is demonstrated numerically with emphasis on characterizing damage in noisy environments, where the wavelet scale Teager-Kaiser energy operator is taken as the benchmark method for comparison. Experimental validations are conducted based on the benchmark data from composite beam specimens measured by a scanning laser vibrometer. Numerical and experimental validations/comparisons present that the introduction of wavelet correlation operator is effective for damage localization in noisy conditions.     

Synthesis of porous oxide ceramics using a soft responsive scaffold

Ceramic materials have significant utility. Developing synthetic protocols that are facile and provide low energy alternatives to traditional methods remains a major driver in materials synthesis. We present here the adaptation of a method recently developed in our group for the synthesis of porous silica using a non-ionic emulsion template. The silicate materials are porous on both the nanometre and micrometre length scales and surface-to-volume ratios may be readily modified by altering the volume fraction of the emulsion template. Switching the silica precursor for an alumina or titania precursor resulted in the formation of porous alumina and titania materials which were prepared as thin films or monoliths. The pores formed in the amorphous alumina materials were ~0.8 μm and ~50 nm, with a primary particle size of 50–100 nm. The titania materials had pores on one length scale only: ~0.8 μm, with a smaller primary particle size of 20–60 nm. As-synthesized materials were investigated using scanning electron microscopy and X-ray diffraction.     

On the inhomogeneous hydration kinetics and stiffness evolution of HNBR‐MgO reactive elastomer composites

Swellable elastomers are widely used in oilfield industry for sealing and zonal isolation applications. These materials need to sustain a large amount of external load after swelling. A newly developed reactive hydrogenated nitrile butadiene rubber (HNBR) based elastomer composite with magnesium oxide (MgO) as filler can swell and stiffen when exposed to water, which makes it ideal for oil field applications. However, both the filler hydration and the stiffness evolution inside this composite material are observed to be highly inhomogeneous even for samples on the length scale of millimeters. To understand this coupled diffusion‐hydration process is critical for applications of these materials with larger length scales. In this work, the hydration kinetics and stiffness evolution of the HNBR‐MgO composite are quantitatively studied on microscopic level. The extent of MgO hydration along the thickness of the sample are measured at the different stage of swelling. These results are used to determine the diffusion coefficient of water inside the composite. The diffusivity increases orders of magnitude after the filler hydration. In addition, the modulus change is non‐proportional to the degree of filler hydration as demonstrated by instrumented grid indentation on the hydrated composites. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133, 43420.     

Model of the nonlinear elastic behavior of a powder composite material. I. Properties of heteroresistant powder materials

A nonlinear elastic isotropic body model is proposed to describe the mechanical behavior of a powder composite material with different properties under tension and compression. The composite is considered as a three-phase body with an elastic matrix, throughout the volume of which pores and rigid inclusions are distributed. The unsymmetrical properties of this material are connected with the nucleation of defects, in particular with decohesion or the formation of discontinuities at the matrix—inclusion interfaces.     

Systematic testing and formal verification to validate reactive programs

The use of systematic testing and formal verification in the validation of reactive systems implemented in synchronous languages is illustrated. Systematic testing and formal verification are two techniques for checking the consistency between a program and its specification. The approach to validation is through specification: two system views are developed in addition to the program, a behavioural specification for systematic testing and a logical specification for formal verification. Pursuing both activities, reactive programs can be validated both more efficiently (in terms of costs) and more effectively (in terms of confidence in correctness). This principle is demonstrated here using the well known lift example.     

Parallel implementation of spectral element method for Lamb wave propagation modeling

The proposed spectral element method implementation is based on sparse matrix storage of local shape function derivatives calculated at Gauss–Lobatto–Legendre points. The algorithm utilizes two basic operations: multiplication of sparse matrix by vector and element‐by‐element vectors multiplication. Compute‐intensive operations are performed for a part of equation of motion derived at the degree of freedom level of 3D isoparametric spectral elements. The assembly is performed at the force vector in such a way that atomic operations are minimized. This is achieved by a new mesh coloring technique The proposed parallel implementation of spectral element method on GPU is applied for the first time for Lamb wave simulations. It has been found that computation on multicore GPU is up to 14 times faster than on single CPU. Copyright © 2015 John Wiley & Sons, Ltd.     

Environmentally controlled emulsion electrospinning for the encapsulation of temperature-sensitive compounds

The introduction of highly volatile fragrances within polymeric nano-scaled fibers is a promising route for efficient and simple encapsulation of temperature-sensitive materials. This work describes the investigation of selected parameters influencing the electrospinning of emulsions of poly(vinyl alcohol) (PVA) and (R)-(+) limonene or hexadecane. Thereby the influence of environmental parameters such as temperature and relative humidity on the fiber structure and encapsulation efficiency (EE) of the fragrance is demonstrated. For that purpose, the electrospinning process was carried out in a climatic cabin in which temperature and relative humidity were controlled. Studied temperatures ranged from 8 to 24 °C and relative humidity varied between 55 and 85 %. The influence of temperature was dependent on the PVA concentration in the emulsion. The relative humidity influenced both the obtained fiber morphology and fragrance EE to a higher extent than the temperature due to the hydrophilic nature of the PVA. This study is of importance when considering the use of emulsion electrospinning for encapsulation purposes.     

Dendrosomes as novel gene porters‐III

BACKGROUND: It was previously reported that dendrosomes, i.e. neutral, biodegradable, covalent or self‐assembled, hyperbranched, spheroidal nano‐particles with a size ranging from 15 to 100 nm, provide a convenient and efficient means of gene delivery into various kinds of cells such as human hepatoma and kidney cells as well as animal models. RESULTS: New studies via circular dichroism show that hydrophilic and amphipathic dendrosomes either do not affect the DNA structure or moderately transform it from B‐ to A‐conformation. Gene delivery into human liver, kidney, and endothelial cells as well as other animal cells like Bowes, U‐937, Raw, CCRF‐CEM, MOLT‐4, K562, Huh‐7 and VERO reveal that the genes are efficiently expressed and in comparison with other gene porters like Lipofectin or bacterial ghosts, do quite well. It is also shown that dendrosomes are able to deliver genes into cells like endothelials that are usually hard to transfect. Cell culture experiments as well as intraperitoneal/intradermal injections of dendrosomes into mice establish their nontoxicity (up to 2.5 mg kg^?1 of animal weight in the latter case). Studies on immunization of BALB/c mice using conventional adjuvants such as aluminium phosphate, C_pG motif and one of the dendrosomes, indicate that the latter leads to the mildest initial response development while exceeding them afterwards. CONCLUSION: CD studies reveal that, owing to the neutrality of dendrosomes, formation of Den/DNA complexes is accompanied by slight structural modifications of DNA cell culture, and animal studies reveal that dendrosomes are inert, non‐toxic and highly efficient gene porters that perform at extremely low doses. In comparison with bacterial ghosts and some common porters, they are efficient in delivery of genes into animals and a variety of cells including those that are usually hard to transfect. Copyright © 2008 Society of Chemical Industry     

Disulfide Bond Substitution by Directed Evolution in an Engineered Binding Protein

Breaking ties : The antitumour protein, neocarzinostatin (NCS), is one of the few drug‐carrying proteins used in human therapeutics. However, the presence of disulfide bonds limits this protein's potential development for many applications. This study describes a generic directed‐evolution approach starting from NCS‐3.24 (shown in the figure complexed with two testosterone molecules) to engineer stable disulfide‐free NCS variants suitable for a variety of purposes, including intracellular applications.