水蛭素聚乙二醇化及其体外抗凝活力分析

Hirudin is the most anticoagulant drug found in nature, but its short serum half-life significantly inhibits its clinical anpplication. The PEGvlation of hirudin, the most promising anticoagulant drug, was performed in this paper. The optimal reaction conditions for PEG ylated hirudin were investigated, wh.en the PEGylation react, on.wasconducted under 4℃ after 10h, in the borate buffer at pH 8.5 .with the molar ratio 230 ： 1 of PEG to hirudin, a higher modification extent was achieved. Finally, the bioactivity of PEGylated hirudin was measured in vitro.Compared with unmodified hirudin, 26% of anti-thrombin activity was retained.     

熔融制片-X射线荧光光谱法测定碳酸盐岩石中主次量元素

采用四硼酸锂和偏硼酸锂2:1混合溶剂熔融制备样片，Zetium X射线荧光光谱仪测定碳酸盐岩石中氧化铝、氧化钙、氧化铁、氧化钾、氧化镁、氧化钠、氧化钡、五氧化二磷、三氧化硫、二氧化硅、二氧化钛、氧化锰等12种组分。重点研究了熔样比、熔样方式、测定条件、基体干扰矫正等，经有证标准物质验证，分析方法的精密度满足DZ/T0130《地质矿产实验室测试质量管理规范》,结果与标准值一致。