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A hydrocarbon degrading bacterium KL2-13 was isolated from ten sites of oil contaminated soil in the Karamay oilfield. It was identified as the Bacillus fusiformis sp. bacterium based on its morphological and physiological characteristics and the 16S rDNA sequence analysis. The factors influencing the hydrocarbon degradation by the bacterium KL2-13 were determined. The test results have showed that the hydrocarbon degrading bacterium KL2-13 requires an optimum pH range of 6-8, and the optimum inoculation quantity is 3%. The low-concentration metal ions Fe2+, Mg2+ and Ca2+ can improve the degradation ability of the bacteria KL2-13.Atoo low concentration of Tween-80 does not show obvious promotion to the de- grading bacterium KL2-13, and an excessively high concentration can decrease the degradation ability of the bacterium, the best dosage of which is 2%. The hydrocarbon degrading rate reached 59.07%±0.37% under the optimum culture conditions. 相似文献
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以微介孔ZSM-5分子筛为载体,采用等体积浸渍法制备了新型有序微介孔非均相Fe-Ce/ZSM-5 Fenton催化剂。采用红外光谱和氮气吸附-脱附对催化剂进行表征,通过H2O2协同降解甲基橙模拟废水,考察了进水pH、甲基橙浓度、催化剂加入量、H2O2浓度及反应温度对催化剂性能的影响。结果表明:制备的Fe-Ce/ZSM-5保持了ZSM-5的骨架结构,且具有较大的比表面积;在进水pH=5.0、甲基橙浓度100 mg/L、催化剂加入量1.0 g/L、H2O2浓度20 mmol/L及反应温度20°C的条件下,甲基橙脱除率为87%,铁离子流失量仅为0.019 mg/L。 相似文献
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从石油烃污染土壤中分离得到菌株L-2,对石油烃(TPH)的降解进行研究。通过16S rRNA鉴定L-2为肠杆菌属。采用基于Box-Behnken设计(BBD)的响应面方法(RSM),考察温度、pH和TPH质量浓度3个自变量对TPH降解率的交互效应,建立二次回归方程预测模型,确定3个实验因素的最佳组合,通过方差分析验证该二次模型的有效性。确定系数R2=0.9943,表明二次模型与实验数据吻合较好。响应面优化得到最佳降解条件:温度为30℃,pH为7.13和TPH质量浓度为3.93g/L,TPH降解率为87.79%。结果表明,温度对TPH降解速率的影响(P<0.0001)较其他两个参数更显著。GC/MS结果表明菌株L-2可降解十六烷(C16)至二十五烷(C25)范围内的TPH组分。 相似文献
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