Octadecylamine as chemical modifier for tuned hydrophobicity of surface modified cellulose: toward organophilic cellulose nanocrystals

Cellulose - A novel, environmentally friendly and simple method for chemical functionalization of microcrystalline cellulose (MCC) to produce organophilic cellulose nanocrystals (CNC-ODA) is herein...     

Bayes minimax estimators of the mean of a scale mixture of multivariate normal distributions

Bayes estimation of the mean of a variance mixture of multivariate normal distributions is considered under sum of squared errors loss. We find broad class of priors (also in the variance mixture of normal class) which result in proper and generalized Bayes minimax estimators. This paper extends the results of Strawderman [Minimax estimation of location parameters for certain spherically symmetric distribution, J. Multivariate Anal. 4 (1974) 255-264] in a manner similar to that of Maruyama [Admissible minimax estimators of a mean vector of scale mixtures of multivariate normal distribution, J. Multivariate Anal. 21 (2003) 69-78] but somewhat more in the spirit of Fourdrinier et al. [On the construction of bayes minimax estimators, Ann. Statist. 26 (1998) 660-671] for the normal case, in the sense that we construct classes of priors giving rise to minimaxity. A feature of this paper is that in certain cases we are able to construct proper Bayes minimax estimators satisfying the properties and bounds in Strawderman [Minimax estimation of location parameters for certain spherically symmetric distribution, J. Multivariate Anal. 4 (1974) 255-264]. We also give some insight into why Strawderman's results do or do not seem to apply in certain cases. In cases where it does not apply, we give minimax estimators based on Berger's [Minimax estimation of location vectors for a wide class of densities, Ann. Statist. 3 (1975) 1318-1328] results. A main condition for minimaxity is that the mixing distributions of the sampling distribution and the prior distribution satisfy a monotone likelihood ratio property with respect to a scale parameter.     

Highly hydrophilic surfaces from polyglycidol grafts with dual antifouling and specific protein recognition properties

Homopolymer grafts from α-tert-butoxy-ω-vinylbenzyl-polyglycidol (PGL) were prepared on gold and stainless steel (SS) substrates modified by 4-benzoyl-phenyl (BP) moieties derived from the electroreduction of the parent salt 4-benzoyl benzene diazonium tetrafluoroborate. The grafted BP aryl groups efficiently served to surface-initiate photopolymerization (SIPP) of PGL. In similar conditions, SIPP of hydroxyethyl methacrylate (HEMA) permitted the production of PHEMA grafts as model surfaces. Water contact angles were found to be 66°, 15°, and 0° for SS-BP, SS-PHEMA, and SS-PPGL, respectively. The spontaneous spreading of water drops on SS-PPGL was invariably observed with 1.5 μL water drops. PPGL thus appears as a superhydrophilic polymer. Resistance to nonspecific adsorption of proteins of PPGL and PHEMA grafts on gold was evaluated by surface plasmon resonance (SPR) using antibovine serum albumin (anti-BSA). The results conclusively show that PPGL-grafts exhibit enhanced resistance to anti-BSA adsorption compared to the well-known hydrophilic PHEMA. PPGL grafts were further modified with BSA through the carbonyldiimidazole activation of the OH groups providing immunosensing surfaces. The so-prepared PPGL-grafted BSA hybrids specifically interacted with anti-BSA in PBS as compared to antimyoglobin. It is clear that the superhydrophilic character of PPGL grafts opens new avenues for biomedical applications where surfaces with dual functionality, namely, specific protein grafting together with resistance to biofouling, are required.     

Evaluation of endothelial cell adhesion onto different protein/gold electrodes by EIS

To study cell attachment to biomaterials, several proteins such as fibronectin, collagen IV, heparin, immunoglobulin G, and albumin have been deposited onto polystyrene adsorbed on a self-assembled monolayer (silane or thiol) on glass or gold, respectively. The different steps of this multilayer assembly have been characterized by electrochemical impedance spectroscopy (EIS). These data are compared to those of adhesion rate, viability percentage, and cytoskeleton labeling for a better understanding of the cell adhesion process to each protein. All the proteins are endothelial cell adhering biomolecules but not with the same features. A linear relationship has been established between adhesion rate and resistance of the endothelial cell/protein interface for all negatively charged proteins.     

Elasticae and inradius

The elastic energy of a convex curve bounding a planar convex body \(\Omega \) is defined by \(E(\Omega )=\frac{1}{2}\,\int _{\partial \Omega } k^2(s)\,ds\) where k(s) is the curvature of the boundary. In this paper we are interested in the minimization problem of \(E(\Omega )\) with a constraint on the inradius of \(\Omega \). In contrast to all the other minimization problems involving this elastic energy (with a perimeter, area, diameter, or circumradius constraints) for which the solution is always the disk, we prove here that the solution of this minimization problem is not the disk and we completely characterize it in terms of elementary functions.     

Endothelial cell adhesion on polyelectrolyte multilayer films functionalised with fibronectin and collagen

The seeding of endothelial cells on biomaterial surfaces has become a major challenge to achieve better haemocompatibility of these surfaces. Multilayers of polyelectrolytes formed by the layerby-layer method are promising in this respect. In this study, the interactions of endothelial cells with multilayered polyelectrolytes films were investigated. The build-ups were prepared by selfassembled alternatively adsorbed polyanions and polycations functionalised with fibronectin and collagen. Anionic poly(sodium 4-styrenesulfonate) and cationic poly(allylamine hydrochloride) polyelectrolytes were chosen as a model system. Elaborated surfaces were characterised by electrochemical impedance spectroscopy and cyclic voltammetry. The modified electrode showed good reversible electrochemical properties and high stability in an electrolyte solution. The film ohmic resistance was highest when the film was coated with fibronectin; the parameters so determined were correlated with atomic force microscopy images. Cell colorimetric assay (WST-1) and immunofluorescence were used to quantify the cell viability and evaluate the adhesion properties. When cultured on a surface where proteins were deposited, cells adhered and proliferated better with fibronectin than with collagen. In addition, a high surface free energy was favourable to adhesion and proliferation (48.8 mJ m⁻² for fibronectin and 39.7 mJ m⁻² for collagen, respectively). Endothelial cells seeded on functionalised-polyelectrolyte multilayer films showed a good morphology and adhesion necessary for the development of a new endothelium.     

Modulation of diffusion with polarized lasers

Laser diffusion is generally used to modify the metallurgical composition at the surface of materials for improving the mechanical properties. Platinum has been diffused into titanium and tantalum sheets in this study, and the concentrations of Pt in the substrates are determined. The concentration of Pt is higher at lower scanning speeds due to higher surface temperature and longer diffusion time than in the case of higher scanning speeds. Additionally, the samples treated with a linearly polarized laser beam exhibit slightly higher concentration of Pt. The enhanced diffusion in the case of linearly polarized laser treatment can be attributed to controlled excitation of the local vibration modes of the atoms in the substrate. The reflectivity of the samples are also measured at the wavelength of 1,064 nm and compared with theoretical results.