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By two-dimensional electrophoretic technique, it was confirmed that BSV RNA-1 and RNA-2 contained circular molecules and that they Were more stable than the ether viroids in thermodynamics. BSV RNAs extracted from individual diseased plants gave only one specific band on analytical polyacrylamide gel, either BSV RNA-1 or RNA-2. No plant which has yet been detected contained both. No variation whatever was observed in the electrophoresis mobility, even over a period of two and a half years for BSV RNA-1 and RNA-2.The calli developed from diseased leaves grew rather slowly compared with healthy ones. The replication of BSV RNA-1 in calli which had continuously grown for 6—8 months was evidenced by the distinct band of BSV RNA-1 on file gels. 相似文献
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实验课是高校培养高层次人才的重要手段,是学生把理论和实践相结合的重要通道.认真上好每一堂实验课,有意识地培养良好的实验习惯,对保证实验的正常进行、确保安全、防止差错有着不可忽视的作用. 相似文献
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