双波长高效液相色谱法分析催泪喷射器内容物

建立了采用双波长,利用简单流动相组成测定催泪喷射器中各有效成分的反相高效液相色谱法。色谱柱为Kromasil C18(250 mm×4.6 mm,5μm),流动相为甲醇：水(80:20,V/V),流速0.8 mL／min,柱温25℃;紫外检测波长为227 nm和300 nm.能够清楚地分辨催泪喷射器内容物中主要的3种成分：邻氯苯亚甲基丙二腈(CS),辣椒素(OC)和二氢辣椒素(DC)．该方法简便快捷,线性关系良好,结果准确可靠,对建立催泪喷射器内容物检测的新质量标准具有参考作用。     

Nano-silica particles enhanced adsorption and recognition of lysozyme on imprinted polymers gels

Molecular imprinting technology has a great potential to be used in protein separation and purification.In this work,lysozyme imprinted polyacrylamide gel was prepared with silica particles as a sacrificial template to generate macro-porosity for fast adsorption.The adsorption equilibrium time and adsorption capacity were 9 h and 56 mg/g respectively,which was 2 h less and 2.3-fold more than polymers without the sacrificial template.In order to test molecular imprinting polymers(MIPs)'selectivity,bovine serum albumin(BSA)was chosen as interferent for binary adsorption tests.In addition,the adsorption selectivity was further investigated using different molar ratios of lysozyme to BSA with fixed total concentration of proteins,as well as using various total concentrations of proteins with an equimolar ratio of lysozyme to BSA.It has been proven that the total concentration of proteins should be larger than1.5×10~(-7) mol/mL,when the molar ratio of BSA to Lyz is 1∶1,in order to effectively separate Lyz from the binary protein mixture.The macro-porous lysozyme molecularly imprinted polymers have less adsorption time,larger adsorption capacity,and better imprinting effect.     

Quantitative Analysis of Components in OC-CS Sprays by High Performance Liquid Chromatography with Double Wavelength UV Detection

The method of reversed-phase high performance liquid chromatography was established for the determination of effective components in OC-CS spray using double wavelength UV detection.The method was utilized under following conditions:a column of Kromasil C18(250 mm × 4.6 mm,5 μm),mobile phase consisting of methanol/water(80 /20) at a flow rate of 0.8 mL/min,column temperature of 25 ℃,and the UV detection at 227 nm and 300 nm.Three key components in OC-CS spray could be distinguished clearly,including o-chlor...     

固相萃取-超高效液相色谱法测定水中四环素类抗生素

本研究建立了测定水中四种四环素类抗生素的超高效液相色谱(ultra-high performance liquid chromatography,UPLC)分析方法。采用固相萃取法提取水中的四环素类抗生素,分别以甲醇和水活化、平衡HLB小柱,用甲醇进行洗脱。在UPLC条件中采用甲醇、乙腈及0.01mol/L柠檬酸溶液作为流动相进行梯度洗脱,外标法定量,分析时间仅为4.5分钟。四种四环素类抗生素的线性关系良好,线性相关系数大于0.999。低中高三个浓度加标回收率分别为56.0%-86.3%,68.0%-78.5%,73.8%-80.4%。土霉素、四环素、金霉素和强力霉素的方法检出限分别为0.71,0.36,0.47,0.88μg/L,与HPLC-MS方法相当。该方法重现性较好,操作简单,分析时间短,可用于高通量水体样品中四环素类抗生素的测定。