High connectivity and directional gene flow in European Atlantic and Mediterranean populations of Ciona intestinalis sp. A

Understanding the factors that cause population divergence has long been of interest to marine biologists in their attempts to interpret the effect of human‐mediated vectors. Broadcast‐spawning species with limited dispersal capability are excellent candidates to measure the present‐day patterns of genetic diversity. The tunicate Ciona intestinalis (Ascidiacea) is comprised of a complex of morphologically cryptic species that form vigorous aggregates in eutrophic habitats (harbors, gulfs and lagoons) where they can compete with the epibenthic community and cause biofouling problems. This study investigated biogeographic variability and migration patterns of C. intestinalis sp. A along Northeast Atlantic and Mediterranean coasts using microsatellite markers. Data presented here on 371 specimens collected from 17 populations reveal high genetic polymorphism, but with a deficit of heterozygote deficiency. Absence of evidence for isolation by distance suggests that the genetic patterns do not reflect the geographic distribution of sampled populations. Substantial gene flow and artificial potential for dispersal boost high levels of within‐population genetic variability and prevent genetic differentiation within and between seas. A predominant eastward migration pattern was revealed by the data set, with very limited opportunity for C. intestinalis sp. A to travel westward. This directional movement indicates that other properties (e.g. habitat quality, genetic traits, mating system, life cycle) may cause adaptive divergence at a large biogeographic scale.     

Comparing seven candidate mission configurations for temporal gravity field retrieval through full-scale numerical simulation

The goal of this contribution is to focus on improving the quality of gravity field models in the form of spherical harmonic representation via alternative configuration scenarios applied in future gravimetric satellite missions. We performed full-scale simulations of various mission scenarios within the frame work of the German joint research project “Concepts for future gravity field satellite missions” as part of the Geotechnologies Program, funded by the German Federal Ministry of Education and Research and the German Research Foundation. In contrast to most previous simulation studies including our own previous work, we extended the simulated time span from one to three consecutive months to improve the robustness of the assessed performance. New is that we performed simulations for seven dedicated satellite configurations in addition to the GRACE scenario, serving as a reference baseline. These scenarios include a “GRACE Follow-on” mission (with some modifications to the currently implemented GRACE-FO mission), and an in-line “Bender” mission, in addition to five mission scenarios that include additional cross-track and radial information. Our results clearly confirm the benefit of radial and cross-track measurement information compared to the GRACE along-track observable: the gravity fields recovered from the related alternative mission scenarios are superior in terms of error level and error isotropy. In fact, one of our main findings is that although the noise levels achievable with the particular configurations do vary between the simulated months, their order of performance remains the same. Our findings show also that the advanced pendulums provide the best performance of the investigated single formations, however an accuracy reduced by about 2–4 times in the important long-wavelength part of the spectrum (for spherical harmonic degrees ${<}50$ ), compared to the Bender mission, can be observed. Concerning state-of-the-art mission constraints, in particular the severe restriction of heterodyne lasers on maximum range-rates, only the moderate Pendulum and the Bender-mission are beneficial options, of course in addition to GRACE and GRACE-FO. Furthermore, a Bender-type constellation would result in the most accurate gravity field solution by a factor of about 12 at long wavelengths (up to degree/order 40) and by a factor of about 200 at short wavelengths (up to degree/order 120) compared to the present GRACE solution. Finally, we suggest the Pendulum and the Bender missions as candidate mission configurations depending on the available budget and technological progress.     

Bacterial killing by Mytilus hemocyte monolayers as a model for investigating the signaling pathways involved in mussel immune defence

The signaling pathways involved in mussel immune defence were investigated utilizing a model of killing of Escherichia coli by Mytilus galloprovincialis hemocytes in a co-culture setting. In particular, the role played by different mitogen activated protein kinases (MAPKs) and by the production of eicosanoids were investigated utilising specific cell permeant, pharmacological enzyme inhibitors. Hemocyte pretreatment with the p38 MAPK inhibitor SB203580 significantly reduced bacterial killing, whereas PD98059 (an inhibitor of ERK--extracellularly regulated kinase--MAPK activation) had no significant effect. Wortmannin also inhibited bacterial killing, indicating a crucial role for PI3-kinase activation in the immune response. Killing of E. coli was also reduced by inhibitors of both PLA2 and cyclooxygenase activities, indicating that eicosanoid production is involved in mediating the response to bacterial challenge. The results demonstrate that bacterial killing by mussel hemocytes is particularly sensitive to inhibitors of the key steps involved in the transduction of bacterial signals into the host cell. Moreover, these data indicate that the hemocyte bactericidal activity can be suitably utilized not only for identifying the signaling pathways involved in the response to bacterial infection, but also as a potential investigative-toxicology model to test drugs and contaminants for their effect on the overall mussel immune defence.     

Structural trends and palaeogeography of the central and western Sicily belt: new insights

The geology of the Sicilian mainland is summarized by N–S geological sections. A continuous late Cenozoic orogenic belt through central and western Sicily resulted from a complex deformative history, recorded by several tectonic events. The deformation mainly involved the sedimentary cover of the old African continental margin, formed in a large basinal area, bordered at its southern margin by a shallow-water carbonate environment attached to Gondwana. The orogenic belt involves a complex architecture of thrust systems, of different size, geometry and palaeogeographical origin. Deformation, which mainly developed in the earlier stages of thrusting in the basinal rock assemblages, mainly gave rise to a stack of three different duplex structures, respectively, composed of Palaeozoic, Mesozoic–Palaeogene and Neogene strata. Large-scale clockwise rotation of the thrusts predated transpressional movements in the hinterland during the latest Miocene to Pliocene. High- angle reverse faults, with lateral components, modified earlier tectonic contacts within the allochthons. Contemporaneous southwards- directed imbrications affected the external southern areas, progressively incorporating foreland and piggyback basirts. The stratigraphic relationships of basin-fills to the tectonic structures reveals that reactivation processes have been active during the last Plio-Pleistocene.     

Predictive optimal control for seismic analysis of non-linear and hysteretic structures

In this paper, an effective active predictive control algorithm is developed for the vibration control of non-linear hysteretic structural systems subjected to earthquake excitation. The non-linear characteristics of the structural behaviour and the effects of time delay in both the measurements and control action are included throughout the entire analysis (design and validation). This is very important since, in current design practice, structures are assumed to behave non-linearly, and time delays induced by sensors and actuator devices are not avoidable. The proposed algorithm focuses on the instantaneous optimal control approach for the development of a control methodology where the non-linearities are brought into the analysis through a non-linear state vector and a non-linear open-loop term. An autoregressive (AR) model is used to predict the earthquake excitation to be considered in the prediction of the structural response. A performance index that is quadratic in the control force and in the predicted non-linear states, with two additional energy related terms, and that is subjected to a non-linear constraint equation, is minimized at every time step. The effectiveness of the proposed closed-open loop non-linear instantaneous optimal prediction control (CONIOPC) strategy is presented by the results of numerical simulations. Since non-linearity and time-delay effects are incorporated in the mathematical model throughout the derivation of the control methodology, good performance and stability of the controlled structural system are guaranteed. Copyright © 1999 John Wiley & Sons, Ltd.     

A numerical study on seismic risk assessment of historic masonry towers: a case study in San Gimignano

After a brief introduction on the research project RiSEM (Seismic Risk of Monumental Buildings), the paper discusses on the seismic assessment of historic masonry towers according to the Italian “Guidelines for the assessment and mitigation of the seismic risk of the cultural heritage” that identifies an analysis methodology based on three different levels of evaluation, according to an increased knowledge of the structure. The RiSEM project aimed at developing and testing innovative and expeditious methodologies (i.e. either without or with a minimal direct contact with the masonry building) to evaluate all the main structural features of the monumental buildings required for the assessment of their seismic safety. As a relevant case study the “Town of Fine Towers”, San Gimignano (Italy), listed under the UNESCO World Cultural Heritage Sites, was selected. The paper summarizes the analyses performed on one of the San Gimignano towers: the Coppi-Campatelli one. The seismic vulnerability of the tower was evaluated with reference to the above guidelines, and the paper reports and critically compares the results obtained for the three levels of evaluation there defined: LV1 (analysis at territorial level), LV2 (local analysis) and LV3 (global analysis).     

What Can be Expected from the GRACE-FO Laser Ranging Interferometer for Earth Science Applications?

The primary objective of the gravity recovery and climate experiment follow-on (GRACE-FO) satellite mission, due for launch in August 2017, is to continue the GRACE time series of global monthly gravity field models. For this, evolved versions of the GRACE microwave instrument, GPS receiver, and accelerometer will be used. A secondary objective is to demonstrate the effectiveness of a laser ranging interferometer (LRI) in improving the satellite-to-satellite tracking measurement performance. In order to investigate the expected enhancement for Earth science applications, we have performed a full-scale simulation over the nominal mission lifetime of 5 years using a realistic orbit scenario and error assumptions both for instrument and background model errors. Unfiltered differences between the synthetic input and the finally recovered time-variable monthly gravity models show notable improvements with the LRI, on a global scale, of the order of 23 %. The gain is realized for wavelengths smaller than 240 km in case of Gaussian filtering but decreases to just a few percent when anisotropic filtering is applied. This is also confirmed for some typical regional Earth science applications which show randomly distributed patterns of small improvements but also degradations when using DDK4-filtered LRI-based models. Analysis of applied error models indicates that accelerometer noise followed by ocean tide and non-tidal mass variation errors are the main contributors to the overall GRACE-FO gravity model error. Improvements in these fields are therefore necessary, besides optimized constellations, to make use of the increased LRI accuracy and to significantly improve gravity field models from next-generation gravity missions.     

Grim4-C1, -C2p: combination solutions of the global earth gravity field

On the basis of the GRIM4-S1 satellite-only Earth gravity model, being accomplished in a common effort by DGFI and GRGS, a combination solution, called GRIM4-C1, has been derivcd using 1° × 1° mean gravity anomalies and 1° × 1° Seasat altimeter derived mean geoid undulations. In the meantime improvements could be achieved by incorporating more tracking data (GEOSAT, SPOT2-DORIS) into the solution, resulting in the two new parallel versions, the satellite-only gravity model GRIM4-S2 and the combined solution GRIM4-C2p (preliminary). All GRIM4 Earth gravity models cover the spectral gravitational constituents complete up to degree and order 50.In this report the emphasis is on the discussion of the combined gravity models: combination and estimation techniques, capabilities for application in precise satellite orbit computation and accuracies in long wavelength geoid representation. It is shown that with the new generation of global gravity models general purpose satellite-only models are no longer inferior to combination solutions if applied to satellite orbit restitution.     

Determination of heavy metals in fresh and sea waters of S. Rossore Park (PISA)

A study was performed in the Tyrrhenian Sea in front of S. Rossore Park (Pisa) to ascertain the degree of Cu, Pb, Cd and Cr pollution and to investigate whether the rivers flowing into the sea may be considered potential sources of seawater contamination. Owing to the variability of such a system, the research, covering a one-year period, involved five surveys on 13 selected sampling stations and two surveys following the floats along the river Arno and its plume. The results refer to the total concentration of the above metals in the water phase after filtration. The content of Cu, Pb and Cd was always found to be higher than in the reference station (about 8 km offshore), generally reaching maximum values around the mouths of the rivers. Concentrations exceeding the minimal risk concentration were observed in the case of Cd. As for Cr, its concentration was always below the detection limit of the analytical method used. Rivers thus appear to be the main sources of coastal metal pollution.By the samplings carried out along the river Arno and its plume (involving 3–5 km from the mouth), it was found that the dilution model represents the concentration pattern for Cu and Pb fairly well over a large range of salinity.     

Improvement of the Excavation Performance of PCD Drag Tools by Water Jet Assistance

The possibility of increasing the performance of mechanical tools with the assistance provided by a high-velocity water jet has been explored in the last 20 years. The results of past research have often been controversial and found to vary within a wide range, mainly due to the different experimental conditions adopted. Number of hypotheses have been advanced to explain the variability of the results as well as to disclose the mechanism of water jet assistance. In this paper, after a discussion of the main issues regarding the assistance of mechanical excavation with water jet, the results of an experimental work carried out at the University of Cagliari are illustrated and discussed. The research was aimed both at studying the processes by which mechanical excavation is improved and at quantifying the increment of the excavation performance parameters with water jet. In particular, the mechanisms involved in the rock–tool–water jet interaction have been studied with the goal of putting into evidence the contribution of the jet both as a way to weaken the rock and to increase the stress leading to scale formation. Better knowledge achieved will be useful for the development of the technology up to a commercial scale.