ISOLATION,CHARACTERIZATION AND EXPRESSION OF THE COMPLEMENTARY DNA FOR HUMAN TUMOR NECROSIS FACTOR (TNF-α)

A cDNA for human TNF-α (615bp) was isolated by means of polymerase chain reaction (PCR) using first strand cDNA from PMA-induced HL-60 cells as template. The result from sequencing the 615 bp cDNA fragment indicated that it corresponded to the entire sequence of mature human TNF coding region. Direct expression of mature human TNF was achieved using a plasmid pHT-1 constructed by ligation of the cDNA and a synthetic DNA. The IPTG-induced bacterial product (hTNF) showed cytotoxicity to mouse L-929 cells. The TNF activity was further identified by neutralization of a specific monoclonal antibody against human TNF-α. Approximately 80,000 units of activity were detected per ml of culture at A600=2.     

HPLC-ICP-MS联用技术测定水样中苯基锡

建立了水样中苯基锡化合物(二苯基锡、三苯基锡和四苯基锡)的高效液相色谱-电感耦合等离子体质谱(HPLC-ICP-MS)的分析方法。水样采用正己烷(含0.01%环庚三烯酚酮)液液萃取,VARIAN Pursuit 5μ-C18反相色谱柱分离,ICP-MS测定。流动相为V甲醇V乙酸(10%,用三乙胺调pH为3.0)=8515。3种化合物在0.5～40μg·L-1范围内均呈良好的线性关系,线性相关系数r0.999;仪器检测限分别为0.16,0.11和0.18μg·L-1;加标回收率在85.3%～109%之间;相对标准偏差8%。方法应用于实际水样中3种苯基锡化合物的分析,结果满意。     

基于改进型傅氏变换轮廓术检测螺纹钢肋径

采用改进型傅氏变换轮廓(I FTP)术,提出了一种实用化的非接触式三维测量系统。通过计算机建模仿真,证明了该技术用于三维测量的可行性。采用了一种局部滤波的方法,通过滤波修正测量结果,使得测量精度明显提高。利用该系统对螺纹钢肋径进行实际测量,获得了良好的实验结果,经与实际值对比,并进行误差分析,验证了该系统具有实用价值和开发前景。     

TDI CCD像元响应不一致性校正算法

线阵TDI CCD遥感相机的不一致性校正对获取高质量的图像来说具有重要意义。主要对TDI CCD像元响应不一致性的校正算法及其硬件实现进行了深入的研究。对CCD的噪声特性进行了详细的分析,给出了像元响应不一致性(PRNU:Pixel-response non-uniformity)的退化模型及校正算法,并对其计算精度进行了分析,在硬件电路上对校正算法进行了验证,详细分析了影响校正效果的各种因素。实测结果表明,校正后图像的信噪比得到显著提高。     

CGNS API和FVM在非结构混合网格计算中的应用

用CGNS API（CFD General Notation System Application Programming Interface）作为非结构混合网格求解器的前处理和后处理,用FVM（Finite Volume Method）作为偏微分方程求解方法．在前处理过程中,用hash表法对内部网格面和边界网格面进行编号,并计算出相应的几何信息,以满足FVM求解器的需要．从FVM求解器计算出来的各种场信息可以写入原来的CGNS文件,该文件可以被许多专业商业后处理软件（如Tecplot,Fluent,CFX等）读取和进行可视化;对于求解器,用基于网格中心的FVM及SIMPLEC（Semi Implicit Methodfor Pressure Linked Equation Consistent）方法求解压力速度耦合．最后给出两个说明算例．     

The Thermal Conductivity Theory of Non-uniform Granular Flow and the Mechanism Analysis

According to the fractal characteristics appearing in non-uniform granular system, we found the fractalmodel to study the effective thermal conductivity in the mixed system. Considering the quasi-equilibrium, we bringforward the fractal velocity probability distribution function. The equipartition of energy is employed to the non-uniform granular system, and the granular temperature is derived. We investigate the thermal conductivity in granularflow due to the movement of the particles, namely the heat transfer induced by the streaming mode only. The thermalconductivity in the mixed system changes with the fractal parameters such as the solid fraction v, structural characterparameter η, and fractal dimension D of size distribution. These parameters depict the characteristics of the thermalconductivity in the actual complex granular system. Comparing our conclusion with the correlative experimental dataand the theoretical conclusion of binary mixture of granular materials, the results can qualitatively confirm the generalityof our prediction on the granular system.     

Curvelet结合图像方向离散度的DSPI无损检测缺陷边缘提取

边缘提取是DSPI无损检测缺陷自动识别的基础.根据人类视觉识别的特点,提出以Curvelet多尺度几何分析为基础进行缺陷图像的边缘提取,为了对DSPI检测过程中因光照不足等原因产生的伪缺陷进行分辨,基于矢量积定义了图像的方向离散度,并与Curvelet多尺度分析方法相结合,在橡胶复合材料DSPI无损检测中进行应用.     

一类三阶自治系统的积分法

研究一类三阶自治系统的积分方法,证明了在一定条件下,这类系统的积分问题归结为求解由该方程的系数所确定的Riccati方程     

小功率空气-氩气混合气冷却ICP中电子密度的测定

在与小功率全Ａｒ－ＩＣＰ类似的条件下,使用顺序扫描等离子体光谱,用Ｓｔａｒｋ加宽法,选择Ｈβ（４８６．１ｎｍ）谱线,测定了空气－氩气混合气冷却ＩＣＰ中在感应线圈上０、２、５、１０ｍｍ处电子密度的侧向分布并将其中部分侧向分布经Ａｂｅｌ变换为径向分布。     

TiO2/SnO2复合空心球在染料敏化太阳能电池中的应用

采用模板辅助法制备了SnO2/TiO2复合空心球,样品直径为1.5~4.0μm,比表面积达到了92.9 m^2·g^-1,复合空心球表现出优越的光散射性能.以这种复合空心球作为染料敏化太阳能电池的光阳极,电池的光电转换效率可达到7.72%,高于SnO2微米球(2.70%)和TiO2微米球(6.26%).此外,以锐钛矿型TiO2纳米晶作为底层,SnO2/TiO2复合空心球作为光散射层制备的双层结构光阳极,电池光电转换效率进一步提升至8.43%.