排序方式: 共有64条查询结果,搜索用时 46 毫秒
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目的探讨外伤性小肠破裂的诊断及治疗方法,减少误诊。方法 对2000~2006年收治的27例外伤性肠破裂患者的临床资料进行回顾性分析。结果 治愈27例。结论 早期诊断,及时手术,选择合理术式,是减少小肠破裂术后并发症的关键。 相似文献
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目的 分析α粒子辐射诱发永生化人支气管上皮细胞恶性转化过程中DNA甲基化谱的变化.方法 分别提取α粒子诱发永生化人支气管上皮恶性转化细胞BERP35T4和对照细胞BEP2D的基因组DNA,用非甲基化敏感酶MseI对基因组DNA进行酶切,然后在酶解后的片段两端连接上连接臂,再用甲基化敏感内切酶进行消化,最终消化产物进行PCR扩增和荧光标记,最后与甲基化芯片进行杂交.杂交结果进行扫描,并对芯片图像进行分析,对芯片上的数据进行归一化处理,最后以差异倍数为1.5的标准来确定差异表达基因.结果 α粒子辐射诱发永生化人支气管上皮细胞恶性转化后,有16个基因发生了甲基化的改变,其中,甲基化上调基因有9个,下调基因7个.鞘氨酸激酶SKIP,蛋白质磷酸酶PPP3CC,蛋白激酶MAP2K6,杀伤细胞免疫球蛋白受体KIR2DLI、KIR2DL4、KIR3DP1,锌指蛋白ZNF493、ZNF100,转录因子NKX2-5、TFAP2D、DR1,钾离子通道KCNJ16,肉瘤抗原CCDC18,以及甘油甲酸酯结合蛋白FNBP1L、同源异形蛋白IRX4、HSF蛋白片段EPB41L3、TCP10蛋白等都发生了甲基化改变.结论 证实了电离辐射能够通过改变细胞的表观遗传修饰而在肿瘤发生中发挥一定的作用.Abstract: Objective To identify the changes of DNA methylation profile in the process of malignant transformation of BEP2D cell induced by α particles.Methods The genomic DNAs were isolated from the malignant transformation BERP35T4 cells and immortalized human bronchial epithelial cell line BEP2D.Genomic DNAs were digested by MseI and ligated of PCR linkers.Methylated DNAs were digested by BstUI and amplified by PCR.The methylated DNA probes were prepared by labeling with Cy3 and Cy5 fluorescence dyes individually and hybridized to the methylation CpG-Island microarray.The hybridization results were scanned and analyzed.Intensity values were quality controlled and normalized.The normalized data were used to identify the differentially expressed genes based on a 1.5 fold difference of the expression level.Results There were 16 genes which showed changes of methylation level in malignant transformation BERP35T4 cells, 9 of them were hypermethylation and 7 were hypomethylation.These genes were including the SKIP gene, PPP3CC gene, MAP2K6 gene, KIR2DL1 gene, KIR2DL4 gene, KIR3DP1 gene, ZNF493 gene, ZNF100 gene, NKX2-5 gene, TFAP2D gene, DR1 gene, KCNJ16 gene, CCDC18 gene, FNBP1L gene, IRX4 gene, EPB41L3 gene, TCP10 gene and so on.Conclusions The DNA methylation might have effects on ionizing radiation drived tumorigenesis. 相似文献
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目的比较超声检查和钼靶显像的结果,评价两种检查方法在诊断乳腺疾病中的作用.方法对我院2000年~2003年经手术病理证实的183例乳腺病人,其中,乳腺增生30例,乳腺腺瘤21例,乳腺恶性肿瘤132例.术前进行彩色多普勒超声和钼靶检查,由多位检查者根据诊断标准和经验作出诊断.结果超声诊断乳腺增生、乳腺腺瘤、乳腺恶性肿瘤的正确率及对乳腺肿块的显示率分别为46.7%、52.4%、62.3%和97.4%,钼靶检查相应值为23.3%、42.9%、59.8%和85.0%.它们之间经x2检验乳腺增生及二者对乳腺肿块的显示率比较具有统计学意义.结论乳腺增生超声显像比钼靶照片具有更高的准确性,当怀疑为乳腺恶性肿瘤时超声与钼靶结合可增加对其诊断的准确性. 相似文献
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