Influence of aeration and agitation modes on solid‐state fermentation of apple pomace waste by Phanerochaete chrysosporium to produce ligninolytic enzymes and co‐extract polyphenols

The effect of different types of agitation (i) continuous agitation (C); (ii) continuous, discontinuous, continuous agitation (C/D/C); (iii) discontinuous, continuous, discontinuous agitation (D/C/D) and aeration (0.87, 1.25, 1.66 vvm) on ligninolytic enzyme production and polyphenolic compounds extraction by solid‐state cultures of Phanerochaete chrysosporium BKM‐F‐1767 was investigated. Higher production of manganese peroxidase (MnP) (1690.3 ± 87.6), lignin peroxidase (LiP) (387.9 ± 14.3) and laccase (898.9 ± 53.3 U gds^?1) and liberation of total polyphenolics (ranging from 12.22 ± 1.1 to 30.12 ± 0.88 mg gallic acid equivalents per gram DW) was obtained after 195, 147, 219 and 204 h of fermentation, respectively, using 1.66 vvm as airflow and (C/D/C) agitation mode. Maximal enzyme production and total polyphenolic content were influenced by aeration, and higher values were obtained using 1.66 vvm as airflow rate, followed by 1.25 and 0.83 vvm, respectively. They were also influenced by agitation, and maximal values were obtained using C/D/C, followed by D/C/D and continuous agitation, respectively. The agitation modes influenced the production of ligninolytic enzymes and simultaneous extraction of polyphenols.     

Modeling VOC Emissions in the High-Purity Oxygen Activated Sludge Process

Mass balances for volatile organic compounds (VOCs) were added to a structured mathematical model of the high-purity oxygen activated sludge (HPO-AS) process. The model was sized to correspond to two large existing HPO-AS treatment plants. The stripping of ten different VOCs was modeled and compared to stripping from conventional air activated sludge process. The results show that the covered aeration tanks can reduce stripping by more than 90%, depending on the specific VOC. If biodegradation is considered, the HPO-AS process degrades more than the conventional process due to the higher liquid-phase concentrations that result because of reduced stripping. The increase in biodegradation depends on the VOCs degradability but should increase to nearly 100% for highly volatile but biodegradable VOCs.     

Glutaminase Production using Zygosaccharomyces rouxii NRRL‐Y 2547: Effect of Aeration,Agitation Regimes and Feeding Strategies

Systematic investigations on the effect of varying dissolved oxygen (DO) concentrations and agitation profiles on glutaminase yield from Zygosaccharomyces rouxii NRRL Y‐2547 were conducted in a batch stirred tank reactor. The agitation rate was varied in the range of 100–400 rpm, while the DO values studied were 20, 40 and 60 %, respectively. Furthermore, a stepwise reduction in DO was investigated wherein the DO concentration was decreased from 40 % for the first 12 h to 20 % for the following 24 h, and finally decreased to 5 % DO up to 54 h (end of batch period). The maximum enzyme yield in all cases was observed after 48 h at 37 °C. An agitation rate of 200 rpm and a three‐stage DO maintenance strategy (40‐20‐5 % DO) gave maximum glutaminase productivity. Yeast extract and glucose feeding increased the enzyme production from 370.75 ± 4.97 units/L to 437.14 ± 4.44 and 417.99 ± 5.82 units/L, respectively.     

Biomass effects on oxygen transfer in membrane bioreactors

Ten biomass samples from both municipal and industrial pilot and full scale submerged membrane bioreactors (MBRs) with mixed liquor suspended solids concentrations (MLSS) ranging from 7.2 to 30.2g L(-1) were studied at six air-flow rates (0.7, 1.3, 2.3, 3, 4.4 and 6m(3)m(-3)h(-1)). Statistical analyses were applied to identify the relative impacts of the various bulk biomass characteristics on oxygen transfer. Of the biomass characteristics studied, only solids concentration (correlated with viscosity), the carbohydrate fraction of the EPS (EPS(c)) and the chemical oxygen demand (COD) concentration of the SMP (SMP(COD)) were found to affect the oxygen transfer parameters k(L)a(20) (the oxygen transfer coefficient) and alpha-factor. The relative influence on k(L)a(20) was MLSS>aeration>EPS(c)>SMP(COD) and on alpha-factor was MLSS>SMP(COD)>EPS(c)>aeration. Both k(L)a(20) and alpha-factor increased with increasing aeration and EPS(c) and decreased with increasing MLSS and SMP(COD). MLSS was found to be the main parameter controlling the oxygen transfer.     

The effect of pre-aeration on the purification processes in the long-term performance of a horizontal subsurface flow constructed wetland

Different conditions (water level, oxygen supply) prevailing in both beds of the Kodij?rve double-bed horizontal subsurface flow (HSSF) constructed wetland (CW) (Southern Estonia; constructed in 1996, total area 312.5 m(2), 40 pe) provide the opportunity to compare how different operational methods have altered the efficiency of the purification processes inside the HSSF CW. In summer 2002 a vertical subsurface flow (VSSF) CW (total area 37.4 m(2)) was added as the first stage of the system. Data from 18 sampling wells installed in Kodij?rve HSSF CW from two periods is compared: 1st period -- January 2000-April 2002 (before the VSSF CW was built); 2nd period --October 2002-December 2004 (after the construction of the VSSF filter). The VSSF CW has remarkably improved aerobic conditions in both beds of the HSSF. Apart from total phosphorus concentrations in the right bed and nitrate nitrogen concentrations in the outflow of both beds, all of the water quality indicators (dissolved oxygen, total suspended solids, biological oxygen demand, ammonia nitrogen, nitrite nitrogen, total nitrogen and total iron) improved after the construction of the VSSF filter. Typically, purification processes in the HSSF CW were dependent on oxygen supply, which was partly influenced by the water level inside the filter beds.     

Treatment of landfill leachate using an aerated, horizontal subsurface-flow constructed wetland

A pilot-scale subsurface-flow constructed wetland was installed at the Jones County Municipal Landfill, near Anamosa, Iowa, in August 1999 to demonstrate the use of constructed wetlands as a viable low-cost treatment option for leachate generated at small landfills. The system was equipped with a patented wetland aeration process to aid in removal of organic matter and ammonia nitrogen. The high iron content of the leachate caused the aeration system to cease 2 years into operation. Upon the installation of a pretreatment chamber for iron removal and a new aeration system, treatment efficiencies dramatically improved. Seasonal performance with and without aeration is reported for 5-day biochemical oxygen demand (BOD(5)), chemical oxygen demand (COD), ammonia nitrogen (NH(4)-N), and nitrate nitrogen (NO(3)-N). Since winter air temperatures in Iowa can be very cold, a layer of mulch insulation was installed on top of the wetland bed to keep the system from freezing. When the insulation layer was properly maintained (either through sufficient litterfall or replenishing the mulch layer), the wetland sustained air temperatures of as low as -26 degrees C without freezing problems.     

压力曝气生物反应器结构设计

对压力曝气生物反应器的结构进行了设计——反应器采用塔式结构,高径比为3∶1,内设环流循环导流筒,采用膜片式微孔曝气器,并对反应器的附件、自动稳定系统和外围设施进行了优化。     

Influence of filtration conditions on membrane fouling and scouring aeration effectiveness in submerged membrane bioreactors to treat municipal wastewater

Membrane fouling and scouring aeration effectiveness were studied using three large pilot-scale submerged membrane bioreactors (MBRs) operated at a series of permeate fluxes, scouring aeration intensities and cyclic aeration frequencies to treat municipal wastewater. The results showed that when operated at the sustainable conditions, the MBRs had a stable reversible fouling resistance. At unsustainable conditions, the reversible fouling resistance increased exponentially as filtration progressed. For each of above two cases, the fouling ratios newly defined by Eqs. (7) and (8) were calculated from the transmembrane pressure increases to compare the relative reversible fouling rates. With the range of sustainable filtration conditions, the fouling ratios at the same reference scouring aeration intensity were found to be proportional to permeate flux. Similarly, the fouling ratios calculated with the same reference permeate flux decreased exponentially with increasing scouring aeration intensity. Moreover, the effects of scouring aeration intensity and permeate flux on the fouling ratios were found to be independent of one another. As a result, an empirical relationship was derived to relate the stable reversible fouling resistance to sustainable permeate fluxes and scouring aeration intensities. Its application was demonstrated by constructing transmembrane pressure contours overlaid with scouring aeration effectiveness contours to aid in the selection of optimal MBR filtration conditions.