嗜酸细胞性胃肠炎三例分析

嗜酸细胞性胃肠炎(eosinophilic gastroenteritis,EG)是一种临床上比较少见的胃肠疾病,是以胃肠道组织中嗜酸细胞(eosinophilic cell,EC)的异常浸润为特征.由于其临床表现为多样性、非特异性,易发生漏诊、误诊.吉林大学第二医院从1995至2002年共确诊3例患者,现将其临床表现、实验室检查、内镜和病理、治疗及预后作一详细探讨.     

父子同患嗜酸细胞性胃肠炎

患者男．27岁，因上腹部痉挛性疼痛，伴发热、腹泻、体重减轻2周就诊。查体：体温38．9℃，脉搏92次／分，呼吸21次／分．血压125／80mmHg。急性病面容，神志清楚，自动体位，步态正常。皮肤粘膜无黄染、皮疹或出血点。全身浅表淋巴结未触及。两侧呼吸动度一致．两肺呼吸音清，未闻及干、湿性罗音。腹无膨隆，腹软，上腹部剑下压痛，无反跳痛，肝、脾未触及，移动性浊音阴性。     

维生素A酸对小鼠囊胚细胞凋亡的影响

目的:探讨全反式维生素A酸(ATRA)对体外培养小鼠囊胚中内细胞群(ICM)和滋养层(TE)细胞凋亡的影响。方法:获取妊娠3.5d小鼠囊胚,分别培养在含0μmol/l、1μmol/l和10μmol/l的ATRA的M199培养基中24h,用带有荧光(FITC)标记的原位末端标记(TUNEL)检测法,观察ATRA对小鼠囊胚细胞凋亡的效应。结果:ATRA可诱导囊胚细胞凋亡,并增强小鼠囊胚Fas蛋白表达。结论:维生素A酸对小鼠胚胎的发育具有细胞毒性作用。     

异维A酸联合白花蛇舌草注射液治疗重度痤疮36例

痤疮是青年人群中最常见的一种皮肤病，重度痤疮常因面部出现较多炎性皮疹、囊肿、结节而使容貌受损，极大地影响患者身心健康。本病治疗方法较多，但疗效不满意。2000年以来，笔者应用口服异维A酸联合白花蛇舌草针肌肉注射治疗本病，取得较好效果，现报道如下。     

托尼萘酸治疗老年戊型肝炎

目的：观察托尼萘酸治疗老年戊型肝炎的安全性及临床疗效。方法：56例老年戊型肝炎病人分为治疗组和对照组，2组病人均给予常规护肝、支持、对症治疗。治疗组36例，男性28例，女性8例：年龄（67&;#177;s11）a。加用托尼萘酸，2片，po，tid。对照组20例，男性16例，女性4例：年龄（66＋12）a，仅给予基础治疗。治疗后4，8wk时比较2组病人的临床指标改变。     

^1H-magnetic resonance spectroscopy screening for animals with acute cerebral infraction suitable forthrombolytic therapy

BACKGROUND: As a non-invasive technique which can provide comprehensive biological information, 1H-magnetic resonance spectroscopy (1H-MRS) may provide valuable reference data for irreversible recovery or reversible changes in ischemic tissue after stroke. OBJECTIVE: To monitor and evaluate the effect of the urokinase thrombolytic therapy after experimental acute cerebral ischemia by 1H-MRS technology and investigate its adaptability. DESIGN: Randomly controlled animal study. SETTINGS: Shenzhen Hospital of Peking University and National Key Laboratory of Pattern and Atom & Molecular Physics, Wuhan Physics and Mathematics Institute, Chinese Academy of Science. MATERIALS: Eleven healthy adult Sprague-Dawley (SD) rats, weighing 260–300 g and of both genders, were supplied by Experimental Animal Center of Tongji Medical Collage, Huazhong University of Science and Technology [SCXK (e) 2004-007]. 4.7T superconducting nuclear magnetic resonance meter was provided by Brucker Company. METHODS: The experiment was carried out in Shenzhen Hospital of Peking University and National Key Laboratory of Pattern and Atom & Molecular Physics, Wuhan Physics and Mathematics Institute, Chinese Academy of Science from August 2003 to December 2005. ① The rats were randomly divided into 30-minute self-thrombo-embolism group (n =6) and 60-minute self-thrombo-embolism group (n =5). Six rats in 30-minute self-thrombo-embolism group were occluded with clot embolus for 30 minutes and 5 rats in 60-minute self-thrombo-embolism group were occluded for 60 minutes. 10 000 U/kg urokinase was dissolved in 2 mL saline and the operation lasted for 5 minutes. ② 1H-MRS was performed before thrombolysis and at 3 hours and 24 hours after successful embolization. The metabolic changes of N-acetyl-L-aspartic acid (NAA)/phosphocreatine (PCr) + creatine (Cr), choline phosphate (Cho)/PCr+Cr and lactic acid (Lac)/PCr+Cr in the region of interests were analyzed. ③ The T2W image was conducted 24 hours after the thrombolytic therapy with TR=500 ms and TE=25 ms. ④ The subjects were sacrificed immediately after 1H-MRS and the brain tissues were cut into pieces and stained with HE method; in addition, pathological changes were observed under optic microscope. MAIN OUTCOME MEASURES: ① Metabolic changes of NAA/PCr+Cr, Cho/PCr+Cr and Lac/PCr+Cr in the region of interests; ② T2W image at 24 hours after the thrombolysis; ③ pathological observation of brain tissue. RESULTS: Eleven rats were all involved in the final analysis. ① Metabolic changes in the region of interests : In 30-minute self-thrombo-embolism group, the Lac peak emerged immediately after the embolism, but the ischemic zone decreased 3 hours after the thrombolytic therapy (0.252±0.01, 0.603±0.01, P < 0.01). Lac/(PCr+Cr) ratio was 0.290±0.01 at 24 hours after thrombolysis, which was higher than that at 3 hours after thrombolysis (P < 0.01). The NAA/ (PCr+Cr) ratio decreased significantly at 3 hours after the thrombolysis as compared with that before thrombolysis (0.922±0.16, 1.196±0.01, P < 0.05). In 60-minute self-thrombo-embolism group, the Lac/(PCr+Cr) ratio was higher at 3 hours after thrombolysis than that before thrombolysis (0.846±0.12, 0.601±0.11, P < 0.05) and the NAA/(PCr+Cr) decreased at 3 hours after the embolism. Fluctuation of NAA/ (PCr+Cr) ranged from 0.68 to 0.75 before thrombolysis and from 0.71 to 0.75 at 3 hours after thrombolysis. ② T2W image: T2W image showed that 2 subjects in 30-minute self-thrombo-embolism group whose Lac/NAA was higher than 0.7 suffered from intracranial hemorrhage. This meant that the subjects with Lac/NAA > 0.7 were more likely to suffer from intracranial hemorrhage. ③ Histological and morphological examinations: Optic microscope demonstrated that interspace surrounding nerve cells was widened at ischemic center; neurons were swelling; nucleus was stained lightly; pyknosis and mesenchymal edema were mainly observed in lateral cortex of brow and vertex and in lateral part of corpus striatum. CONCLUSION: ①Compound parameters in ischemic area before thrombolysis should be regarded as an important predicting marker for thrombolytic therapy, effect evaluation and termination. ② 1H-MRS combining with other imaging technique is a detecting way for screening cases who are suitable for thrombolytic therapy.     

骨髓间充质干细胞在慢性马兜铃酸肾病肾组织动态分布的实验研究

目的 研究雄性大鼠骨髓间充质干细胞（MSCs）经尾静脉移植到慢性马兜铃酸肾病（CAAN）雌性大鼠后，在CAAN肾脏的分布路线和增殖规律。     

诺丽青果化学成分研究

目的 研究诺丽Morinda citrifolia青果的活性成分.方法 诺丽青果干燥粉末经95%乙醇提取浓缩,浸膏用水分散.分别用石油醚、醋酸乙酯和正丁醇萃取,醋酸乙酯部分经硅胶,Sephadex LH-20等材料分离,波谱学方法鉴定化合物结构.结果 共分离得到了15个化合物,鉴定了其中14个化合物,分别为香兰素(Ⅰ)、乌苏酸(Ⅱ)、β-谷甾醇(Ⅲ)、morindone-5-methylether(Ⅳ)、5,15-dimethyl morindol(Ⅴ)、乌苏酸内酯(Ⅵ)、6-甲氧基-7-羟基香豆素(Ⅶ)、香兰酸(Ⅷ)、scutellaric acid(Ⅸ)、rotungenic acid(Ⅹ)、americanin A(Ⅺ)、barbinerric acid(Ⅻ)、6,7-二羟基香豆素(ⅩⅢ )和胡萝卜苷(ⅩⅣ ).结论 化合物Ⅵ和Ⅷ为首次从该种中分到.     

辛芩片薄层鉴别与HPLC含量测定

目的:用薄层色谱法和高效液相色谱法对辛芩片中有关物质进行定性定量检测.方法 :用薄层色谱法对辛芩片中细辛、白芷、黄芪、黄芩、苍耳子五种药材进行鉴别;以高效液相色谱法测定辛芩片中黄芩苷、马兜铃酸A的的含量.结果 :五种药材的薄层鉴别均呈阳性;HPLC测定出黄芩苷的含量每片不低于14.0mg,马兜铃酸A的的含量每片不大于0.015mg.结论 :两种方法都可用于辛芩片中有关物质的检测,为该制剂的质量控制指标提供了实验依据.