健脾补肾疏肝解毒法治疗肝癌体会

原发性肝癌,属于中医学"积聚"、"黄疸"、"臌胀"、"胁痛"等范畴,具有起病隐匿、潜伏期长、高度恶性、进展快、侵袭性强、易转移、预后差等特点.随着近年来综合治疗概念的确立,中医药更加积极参与了肝癌临床防治,使总体疗效得到了进一步的提高.现将我们运用中医药治疗原发性肝癌的体会介绍如下.     

Mediation of mitochondrial translocator protein by tanshinone ⅡA in apoptosis of HepG2 cells北大核心CSCD

Aim To investigate the role of mitochondrial translocator protein(TSPO)in the apoptosis of HepG2 cells induced by tanshinone IIA(Tan II A)and the involved mechanism. Methods Following the HepG2 cells treated with Tan ⅡA at 2.5, 5 and 10 μmol·L-1, the cell viability was determined by MTT assay, and intracellular ATP content was determined by luciferin-luciferase method. Oxygen utilization was measured polarographically with a Clark oxygen electrode. Cell apoptosis was determined by Hoechst 33342 staining and flow cytometry. The mitochondrial membrane potential was assessed with JC-1 staining. The intracellular distribution of TSPO was examined by TSPO immunostaining, and the expressions of TSPO, Cyto C, caspase-3, caspase-9 were determined by immunoblotting analysis. Results Tan II A inhibited the proliferation of HepG2 cells in a dose-and time-dependent manner. The treatment with Tan II A inhibited ATP production and oxygen utilization of mitochondria. In addition, Tan ⅡA enhanced TSPO expression and accumulation in nuclei and up-regulated the expression of Cyto C, caspase-3 and caspase-9. Conclusions Tan II A induces the apoptosis of HepG2 cells, which may be related to the TSPO-mediated mitochondrial dysfunction. © 2023 Publication Centre of Anhui Medical University. All rights reserved.