沿海某地海鱼弧菌伤口感染能力研究

目的 研究从某沿海海水中分离出的海鱼弧菌的致病性。方法 ①毒力实验组：35只二级昆自小鼠随机分为4组,实验组腹腔注射海鱼弧菌,阳性对照组腹腔注射标准金黄色葡葡球菌、标准大肠杆菌,阴性对照组腹腔注射无菌生理盐水。②伤口感染实验组：35只无特定病原体小鼠,腿部致伤后随机分为4组,实验组(浸泡含有细菌的人工海水)和阳性对照组(含有标准金黄色葡萄球菌、标准大肠杆菌),阴性对照组(浸泡无菌人工海水)。观察小鼠一般状况、血常规、血培养、脏器细菌培养、伤口分泌物培养、脏器和伤肢病理光镜检查,并将鼠体内分离的菌株和实验前菌株进行核酸指纹图比较分析,确认细菌来源。结果 ①毒力实验：海鱼弧菌组(海鱼组)腹腔注射细菌悬液后一般情况差,持续24h,病理学检查见多脏器水肿、变性。②伤口感染实验：实验组和阳性对照组伤口感染阳性率为100％,病理学检查海鱼组及阳性对照组伤肢皮下及横纹肌可见大量嗜中性粒细胞浸润,有蜂窝织炎形成。结论 自某沿海海域分离出的海鱼弧菌在10^6CFU／ml时,具有感染能力,可致实验鼠的伤口感染。     

犬肠管破裂伤合并海水浸泡后血流动力学及病理学变化

目的 分析犬肠管破裂伤合并海水浸泡后血流动力学及病理学变化的特点,为海战肠管破裂伤的早期救治提供理论依据。方法 成年杂种犬30只,制作肠管破裂伤动物模型,随机分为海水浸泡组(n=10)、生理盐水浸泡组(n=10)和对照组(n=10)。前两组伤后分别浸泡于人工海水和生理盐水中,对照组单纯致伤不浸泡。观察犬肠管破裂伤后在血流动力学及病理学的变化规律。结果 肠管破裂伤合并海水浸泡后出现明显的平均动脉压(MAP)、肺动脉嵌压(PAWP)、中心静脉压(CVP)和心排出量(CO)下降等血流动力学紊乱及重要器官损伤的病理学改变。生理盐水浸泡组和对照组在致伤前后无明显血流动力学及病理学变化。结论 海水浸泡是导致犬肠管破裂伤合并海水浸泡后血流动力学紊乱的主要因素。     

异基因骨髓移植术后1例尸检报告

急性白血病患者进行异基因骨髓移植术治疗发展较快,移植后合并粟粒性绪核的病例实属罕见,现报告1例。病历摘要患者男性,36岁,籍贯北京。于1986年10月27日心慌、气短、下肢浮肿急诊入院。患者于1985年1月确诊为急性早幼粒细胞白血病,4月7日行异基因骨髓移植,骨髓供应者为患者胞姐。供受体之间 HLA、ABC DR 位点相同,MLC 无反应。移植后病程顺利,8月10日因高热1周伴咳嗽再次入院。     

兔烧伤合并海水浸泡血流动力学变化

目的 建立兔烧伤合并海水浸泡的实验模型，观察实验动物血液动力学变化。方法 实验动物烧伤后随机分为单纯烧伤组（B组,n=6）和烧伤合并海水浸泡组（B＋I组，n=12)。烧伤合并海水浸泡组烧伤后置于人工配制的海水中，监测血液动力学指标变化，直至动物死亡。单纯烧伤组置于干燥处同时监测相同指标。结果 烧伤合并海水浸泡组血液动力学指标紊乱明显重于单纯烧伤组，死亡率明显高于单纯烧伤组，平均存活时间为6.9h。有严重的体温过低。结论 烧伤合并海水浸泡可引起机体的严重的病理生理变化，血液动力学变化明显重于单纯烧伤组，表明海水浸泡使烧伤休克加重，导致实验动物存活时间显著缩短。     

胰岛内分泌肿瘤中α-HCG蛋白及mRNA表达的研究──应用免疫组化及原位PCR技术对52例的观察

应用免疫组化对52例胰岛内分泌肿瘤（PET）α－HCG蛋白表达进行观察；并用原位PCR技术中对其α-HCGmRNA的表达进行观察。结果发现α-HCG在各类正常胰岛细胞中未见蛋白及基因的阳性表达。在良性及恶性PET中α-HCG蛋白检出率分别为17．9％（5／28）及58．3％（14／24）。在α－HCG抗原阳性的PET中应用原位PCR技术均可见有α－HCGmRNA的表达。说明该类肿瘤细胞具有合成α-HCG的能力。对α－HCG在PET中表达的临床病理意义进行了研讨。     

海水浸泡腹部开放性损伤对大鼠体液代谢的影响

目的：了解腹部开放性损伤的大鼠经海水浸泡不同时间后机体体液代谢的变化。方法：观察雄性Ｗｉｓｔａｒ大鼠腹部开放性损伤条件下,经海水浸泡不同时间后大鼠血浆的水、电解质、晶体渗透浓度、红细胞体积分数及血红蛋白含量等的变化,并设单纯腹部开放性损伤组及生理盐水浸泡组作为对照。结果：与单纯腹部开放性损伤组及生理盐水浸泡组相比,高渗海水进入腹腔后１ｈ,大鼠血浆Ｎａ＾＋,Ｋ＾＋,Ｃｌ＾－浓度即显著升高,ＨＣＯ３＾     

大肠腺瘤及大肠癌细胞动力学的免疫组织化学观察

收集的活检标木包括:正常大肠粘膜5例、大肠腺瘤18例、大肠癌8例,应用体外组织培养技术掺入5-溴脱氧尿核甙(BrdUrd),并以Brd Urd单克隆抗体免疫组织化学方法检测具有分裂能力的阳性细胞,计算标记指数(LI),观察细胞增殖带范围。结果发现:大肠腺瘤及大肠癌的LI明显高于正常粘膜(P<0.001)°腺瘤伴不同程度异型增生的LI也不同,中、重度异型增生高于轻度异型增生腺瘤(P<0.05),而与腺癌无明显差异。正常粘膜增殖带局限于隐窝下半部,而腺瘤及大肠癌的具有分裂能力的细胞则散布整个肿瘤细胞区域。这一结果证实从异型增生发展到癌的组织发生过程。     

THE ENDOCRINE CELLS OF EXTRATUMORAL MUCOSA IN RELATION TO THE CARCINOMA IN STOMACH

One hundred and sixty-one surgically resected gastric carcinomas and 5 stomachs obtained at necropsy were evaluated using anti chromogranin A (CgA). CgA-Positive cells were found in 55 of 161 gastric carcinoma. Endocrine cells (EC) of the 55 cases of rumors and extratumoral mucosa and 5 normal gastric mucosa were distinguished by immunoreastive gastrin (CAST), somatostatin (SS), serotonin (SERO), calcitonin (CT), ACTH, human chorionic gonadotropin (HCG) , glucagon (GLU) , pancreatic polypeptide (PP), bombesin (BOM). Each type EC in extratumoral mucosa was quantitated by Image analyser. TWO variants of changes of EC were identified on the basis of presence or absence of associated antral atrophy. In the group of antral atrophy, amount of G cells decreases than normal control (58. 7mm2, P<0. 001); D cells and BOM-positive cell also decrease; GLU-positive cells distributing in glands of intestinal metaplasia increase (18. 6/mm2, P<0. 001) in antral mucosa. There is not obvious change in the average thickne     

Effects of seawater immersion combined with open abdominal injury on the expression of NF-κB,and IκBαof small intestine in rats

Objective To observe effects of seawater immersion combined with open abdominal injury on the expression of NF-κB,and IκBαas well as the change pattern in rats. Methods Ninety-one Wistar rats were randomly divided into 3 groups: the control group (n =7), the open abdominal injury group(n =42) and open abdominal injury combined with 1-hour seawater immersion group ( n =42). The expression of NF-κB,andIκBαin small intestine tissues was measured by Western blot and statistical analyses were also made in the study. Results The expression of NF-κB,in the seawater immersion combined with open abdominal injury group increased significantly 3 hours after injury, when compared with that of the open abdominal injury group(P<0. 05), whereas the expression of NF-κB, of the pure injury group was slightly lower than that of the control group, but no statistical differences could be seen between them(P>0.05). The change pattern in the expression of IκBαwas quite the opposite to that of NF-κB. Conclusions NF-κB seemed to be rapidly and persistently involved in the whole inflammatory response to trauma induced by opened abdominal injury and seawater immersion, when a comparison was made with the pure open abdominal injury group. Injuries for the rats in the open abdominal injury combined with seawater immersion group were serious, and the feedback mechanism for NF-κB was not established for quite a long time.