胎儿生长受限仔鼠脂肪组织中葡萄糖转运蛋白4的表达和细胞转位变化

Objective To investigate the effects of fetal growth restriction (FGR) on the expressions and translocation of glucose transporter 4 (GLUT4) in adipose tissue of male offsprings and its relationship with insulin resistance in FGR. Methods Male 8-week-old offspring from maternal with protein-malnutrition or normal diet were studied. The weight of rats at 8 weeks of age were obtained and insulin resistance index (IRI) was examined at 4 weeks and 8 weeks of age. Perinephric fat pads were harvested to measure the expression of GLUT4 mRNA by fluorescent quantitative RT-PCR and the GLUT4 protein level and insulin-stimulated transloeation were assessed by Western blot. Results Birth weight of FGR animals were significantly lower than that of control (t=6.399, P<0.01), but surpassed the control at 8 weeks with increased fasting IRI(P<0.05). The GLUT4 mRNA expression in adipose tissue of FGR rats (0.36± 0.04) was lower than that of control (1.01±0.11) (t=2.854, P<0.05). Declined total GLUT4 protein concentration(913.47±87.32) was also noted in the FGR group compared with the controls (1248.30± 131.52) (t=2.617, P<0.05). After administration of insulin, plasma membrane associated-GLUT4 concentration significantly increased to 897.03±102.87, which was equal to 2.12 times of the basal level (423.05±41.26) in control rats(t=4.759, P<0.01). However, insulin-responsive GLUT4 transloeation was markedly blunted in FGR rats(525.12±54.96) compared with controls, only increased to 1.38 times of the basal level (379.57±38.71)(t=2.083, P<0.05). Conclusions Decreased GLUT4 expression and insulin-stimulated translocation in adipose tissue of the male FGR offspring might be caused by exposure to protein malnutrition during pregnancy, which may disturb the uptake and utilization of glucose and subsequently be related to diabetes mellitus in later life.     

实行人本管理加强院前急救队伍建设

探讨针对院前急救的行业特点实行人本管理的新思路.从正反两个方面分析实行人本管理的重要性和必要性.坚持以人为本的管理理念,加强院前急救队伍建设,提高院前急救能力,为保障人民健康服务.     

EGB对脑缺血再灌注大鼠脑组织GFAP表达的作用

目的:观察银杏叶提取物(extract of Ginkgo biloba,EGB)对大鼠局灶性脑缺血再灌注梗死区胶质纤维酸性蛋白(GFAP)表达的影响。方法:大脑中动脉插线法制作大鼠局灶性脑缺血再灌注模型。观察各时间点大鼠神经功能缺损程度,并应用Metamoph图像分析系统对结果进行分析。结果:EGB药物组神经功能评分较缺血再灌组好(P<0.05),GFAP阳性细胞于脑缺血2h再灌注24h后即已出现,48、72、96h阳性细胞表达量增加,其中以72h为最多,EGB可抑制缺血后GFAP的表达(P<0.05)。结论:局灶性脑缺血再灌注后,可诱导脑组织GFAP表达增强,EGB可抑制脑缺血后星形胶质细胞GFAP的高表达,提示EGB可能对缺血诱导的星形胶质细胞活化具有抑制作用,这可能是EGB抗脑缺血损伤保护神经元作用的机制之一。     

化妆品中肾上腺素的含量测定

目的：建立一种快速、灵敏检测化妆品中肾上腺素的方法。方法：分光光度法。结果：肾上腺素浓度在0.004～0.2 g/L范围内呈线性关系,回收率在96.0%～101.2%之间,检测下限为0.002 g/L。结论：该方法操作简便,重现性好,线性范围宽,可用于化妆品中肾上腺素的测定。     

针灸治疗颈肩综合征疗效观察

颈肩综合征是一种以颈、胸椎关节失稳及其周围肌肉、韧带劳损造成的颈后、肩背部疼痛不适甚至颈部活动受限等一系列症状的一种病症。我采用针灸治疗辅以颈胸椎手法复位，取得很好疗效。现将收治260例总结如下：     

抗大鼠肾小球基底膜肾炎模型建立方法的改进

目的探讨快速建立抗大鼠肾小球基底膜（glomerular basement membrane，GBM）肾炎动物模型的方法。方法30只正常SD大鼠随机分为三组，肾炎模型A、B组和正常对照组。肾炎模型A、B组大鼠一次性尾静脉注射兔抗GBM血清；正常对照组，尾静脉注射等量正常兔血清。肾炎模型A组大鼠尾静脉注射兔抗大鼠GBM血清前一周，通过足垫皮内注射正常兔血清进行预免疫。上述三组大鼠于尾静脉注射兔血清后第2、7、14和21天，检测24h尿蛋白、血肌酐、血尿素氮含量；第21天处死大鼠取肾组织观察肾小球和肾小管的病理变化。结果肾炎模型A、B组，尾静脉注射兔抗大鼠GBM血清后，24h尿蛋白，血肌酐和血尿素氮含量均明显升高（P〈0．01），A组升高程度均较B组明显（P〈0．05）。光镜检测A组新月体形成率较B组明显升高，肾小管内蛋白管型较B组多，部分肾小球不完全纤维化，B组肾小球未见纤维化。对照组上述指标均无明显变化。结论大鼠注射兔抗GBM血清之前，进行正常兔血清预免疫，可以更快诱导大鼠产生抗GBM肾炎。     

小半夏合苓桂术甘汤治疗急性胃炎理论探析

现代医学认为,急性胃炎是由多种原因引起的急性胃黏膜炎症,临床上急性发作,常表现为上腹部症状,并将其分为数个亚型.然而,从中医学角度来分析现代医学所述的急性胃炎,临床上的急性恶心、呕吐、胸脘痞闷等症可与之互参,且多伴有眩晕、乏力等全身症状.     

经颈内动脉注射pLXSN-bcl-2对脑局灶性缺血再灌注大鼠脑组织的影响

目的:研究经颈内动脉注射pLXSN-bcl-2对大鼠脑缺血再灌注损伤后的影响。方法:51只健康成年Wistar大鼠随机分对照组、空质粒组、bcl-2组,每组分缺血再灌注9,24h两小组。采用线栓法制作大脑中动脉梗死模型,2h后实现再灌注。3h后经颈内动脉注射质粒pLXSN,pLXSN-bcl-2。检测缺血再灌注24h后大鼠脑梗死体积,及各组大鼠脑内bcl-2、caspase-3的表达情况和神经细胞凋亡情况。结果:bcl-2组缺血再灌注24h的脑梗死体积最小;bcl-2组在缺血再灌注9,24h后bcl-2阳性表达明显升高;缺血再灌注24h caspase-3蛋白表达及凋亡细胞数明显降低,缺血再灌注9h后与其他组之间无差异。结论:脑缺血再灌注3h后经颈内动脉注射pLXSN-bcl-2可以增加bcl-2在脑内的表达,并在缺血再灌注24h后通过抑制caspase-3的表达,抑制神经细胞凋亡,减少脑梗死体积。而在缺血再灌注9h尚未表现出明显的神经保护作用。     

曲克芦丁致全身肌肉急烈刺痛1例

患者,男,51岁,2005年6月13日因口齿不清,右侧肢体活动不自如20 h来我院就诊并入院治疗。既往有一年余高血压病史,长期自服阿司匹林50 mg qd、美托洛尔25 mg bid。体检:T 36．3℃,P 76次·min-1,R 20次·min-1,BP 110／80 mmHg,发育营养正常,急性病容,口齿不清,不能行走,扶人病房,神清,查体合作。急诊CT示:双侧基底节区及左侧放射冠区腔隙灶;实验室检查:D2-聚体阳性(++ +),诊断为脑梗死。治疗方案:第1组注射用阿魏酸钠0．3 g加入5％葡萄糖注射液500 ml,ivd qd;第2组曲克芦丁注射液0．5 g加入5％葡萄糖注射液250 ml,ivd qd;第3组能量输液;口服尼莫地平片20 mg,tid;益脉康片40 mg×2片, tid。5 d后病情稳定,但患者有时出现呕吐现象,另加注射用