Critical-point drying versus freeze drying for scanning electron microscopy: a quantitative and qualitative study on isolated hepatocytes

Critical-point drying and freeze drying were compared both quantitatively and qualitatively as preparative procedures for scanning electron microscopy. Isolated hepatocytes were used as model cells. Nomarski differential interference contrast microscopy was used for light microscopic measurements of the hepatocytes in the unfixed, the glutaraldehyde fixed, the glutaraldehyde + OsO₄ fixed, the critical-point dried and the freeze dried states. Critical-point dried hepatocytes were found to shrink to 38% of glutaraldehyde + OsO₄ fixed volume, whereas optimal freeze dried hepatocytes (frozen in water saturated with chloroform and freeze dried at 183 K for 84 h) were found to shrink to 51% of glutaraldehyde + OsO₄ fixed volume. Transmission and scanning electron micrographs of the critical-point dried cells showed well-preserved ultrastructure and surface structure. Micrographs of the freeze dried cells showed ultrastructure destroyed by internal ice crystals and surface structure destroyed by external ice crystals. Double-fixed isolated hepatocytes were shown to swell during storage in buffer and to shrink during storage after critical-point drying. For low magnification scanning electron microscopy (up to about 3000 times) both critical-point drying and freeze drying can be used. However, for high magnification scanning electron microscopy, critical-point drying is superior to freeze drying.     

文72块沙三中油藏天然气驱可行性试验研究

根据文南油田72块沙三中油藏的性质和流体特征，通过室内细管驱替试验和长岩心驱替试验研究，表明注入天然气组成达到一定比例和注入气和温度大于某一临界值，就可以对该油藏进行注烃类气体混相驱，说明注气开发是可行的，并能够提高油藏的采收率。     

Novel application of radiotracer techniques for corrosion studies of zinc and aluminium in acidic medium

The adsorption of sulphate ions on Zn and Al was studied in 0.5 M NaClO₄ supporting electrolyte at various pH values in the range of pH=2-7 by radiotracer techniques. It was found in both cases that the adsorption of sulphate passes over maximum in the pH range studied.The phenomena observed are interpreted by the assumption that the main component of the overall process is anion adsorption on the protonated oxides/hydroxides formed as a result of corrosion. At low pH values the steady state coverage with respect to these products should be very low owing to their dissolution; consequently the extent of anion adsorption induced must also be very low. At higher pH values where no protonation occurs the adsorption of anions decreases significantly.     

松辽流域水利规划及战略研究总体思路

在总结水利规划工作经验教训的基础上,结合松辽流域现在水利工作实际,进行了水利规划及战略研究工作,提出切实可行的解决途径,并着重阐述了松辽流域水利规划和战略研究的总体思路.     

企业滋生腐败与防范中的问题研究

分析了产生腐败的内在因素和外在环境;提出了企业在反腐败工作中存在的自律方面的问题、权利作用问题和监督部门的设置问题,并提出建立“分离分立不分家,制约制衡不掣肘”的防范机制。     

跋山水库抛石挤淤压重的试验研究

通过对水库坝前沉积的淤泥进行详细的试验分析,摸清淤泥的物理性质、状态指标,找出一条适宜的施工工艺,进行淤泥清淤,既能保证工程质量,又能加快进度,节省投资,为山区丘陵地区的水库加固提供新的经验。     

对国际岩石力学委员会J_V公式的质疑

通过对国际岩石力学委员会JV 公式的论证 ,指出了该公式不符合数理逻辑。结合工程勘察实践 ,拟建了Ky新公式 ,提高了工程岩体完整程度评价方法的科学性。     

钢纤维喷射混凝土作为面板材料的可行性研究

钢纤维混凝土作为一种新型增强材料，以其较好的受力变形特性在水利水电，交通，海岸防护，港口及军事等诸多工程中得到了广泛的应用。针对混凝土面板堆石坝面板的受力变形特点，为改善水布垭面板堆石坝面板的受力条件，提高其防渗能力，同时方便施工，现提出采用钢纤维喷射混凝土作为水布垭面板堆石坝面板材料的新构想，并通过对钢纤维喷射混凝土的物理力学特性及堆石坝面板受力变形进行分析，为钢纤维喷射混凝土在水布垭面板堆石坝面板中的实际应用提出了建议方案。     

Laboratory study of property-modified prebaked carbon anode and application in large aluminum electrolysis cells

A kind of complex additive mainly containing Al, Mg, F, and O was prepared. The synthetical performances of the property-modified prebaked anodes containing additives were tested in laboratory. On the basis of ideal testing results obtained, a large number of industrial prebaked property-modified anodes are prepared in a large-scale aluminum company. Further more, they are all used in 160 kA prebaked anode aluminum electrolysis cells. The statistic result show that, compared with common anodes, the property-modified ones enhance current by 11.6 kg per ton aluminum averagely.     

变频电液速度控制系统的实验研究

本文基于变频调速技术,构建了一个较合理的变频电液速度控制系统,并采取模糊控制算法进行试验,实现了快速维持负载突变时液压马达转速的稳定。