2016-2018年郑州市男男性行为人群哨点监测结果及HIV感染影响因素分析

目的 了解郑州市男男性行为(men who have sex with men,MSM)人群艾滋病哨点监测结果及人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染影响因素,为针对MSM人群科学防治艾滋病提供依据。方法 收集郑州市2016-2018年MSM艾滋病哨点监测问卷及检测数据,对资料进行整理,并利用SPSS进行统计学分析。结果 2016-2018年共监测838例,3年HIV抗体阳性率无统计学差异(P>0.05);梅毒抗体阳性率逐年降低(〖XC小五号.EPS;P〗趋势=8.37,P=0.004);艾滋病相关知识知晓率分别为63.46%、69.64%、74.83%,3年总体知晓率为69.57%,各年知晓率逐年升高(〖XC小五号.EPS;P〗趋势=8.45,P值=0.004);多因素分析显示,最近6个月发生同性性行为、最近一周同性肛交次数2次及以上、梅毒阳性为HIV感染促进因素;最近6个月与女性发生性行为、最近6个月发生同性行为坚持使用安全套、大专及以上、接受过干预安全套或艾滋病咨询与检测为减少HIV感染的保护因素。结论 郑州市MSM人群HIV阳性率较高,艾滋病相关知识知晓率不高,应依据HIV感染影响因素采取有效防治措施进行干预。     

垃圾清运工眼及呼吸系统损害调查

对垃圾清运工人眼及呼吸系统损害情况进行调查,结果显示所有眼结合膜及呼吸道刺激症状发生率显著增高;肺功能也受到损害,敏感指标是FVC和FEV1.0。     

胃癌细胞脱落抗单克隆抗体的制备

以无血清培基培养胃癌细胞M85,收集脱落抗原。以胃癌细胞及其脱落抗原免疫Balb/C小鼠,取其脾细胞与小鼠骨髓瘤细胞SP2/0进行融合。经选择培养后,以免疫荧光染色及脱落抗原固相ELISA进行双重筛选。三次克隆化后获得稳定地分泌单克隆抗体(MCAb)的杂交瘤系S_1、S_2及S_3。McAb S_1、S_2及S_3主要与胃癌细胞系呈阳性反应而不与正常细胞及经检测的其它肿瘤细胞起反应。其对应抗原定位于靶细胞的膜上且可释放于培养上清中。     

抗胃癌单克隆抗体的研制—几种免疫流程的比较

本文对用于制备抗胃癌单抗的三种免疫流程进行了比较,并对所获得的单抗的选择性进行了评价。免疫流程Ⅰ以胃癌细胞系MGC 803为免疫原制备出单抗PD4。免疫流程Ⅱ采用胃癌细胞系M85以抗正常胃粘膜组织及混合淋巴细胞血清包被处理作为免疫原,制备出单抗     

MONOCLONAL ANTIBODIES AGAINST HUMAN GASTRIC CANCER

Spleen cells from Balb/c mice immunized with five human gastric cancer cell lines in sequence were fused with murine myeloma cell line SP2/0, and hybridomas 3F4, 3G9 and 3H11, secreting monoclonal antibodiees (MoAbs) against gastric cancer, were obtained through selective culture and screening. These MoAbs have both good selectivity and a high positive rate of reaction for gastric cancer, reaching 5/5 and 84.8% to 93.5% for gastric cancer cells and tissues respectively. The reaction of MoAbs with normal cells and tissues was neglible.The corresponding antigens of the MoAbs were sensitive to digestion by trypsin and pronase and resistant to treatment with sodium periodate, indicating their nature as proteins. The antigen 3G9 could be visualized with Western blotting as two bands with molecular weights of 100KD and 70KD, however no band was found for antigens 3F4 and 3H11. There was a high expression of antigens in the majority of gastric cancer cells and tissues independent of his-topathological type of     

酶联免疫吸附试验(ELISA)在抗MGC 803单克隆抗体的筛选及检测中的应用

自Kohler及Milstein建立淋巴细胞杂交瘤技术以来,该技术已广泛地用于肿瘤抗原的研究。其中,建立一种灵敏、特异简便、快速的筛选及检测方法是应用该技术的关键之一。常用的方法中以固相放射免疫法(RIA)及酶联免疫吸附试验(ELISA)较理想。后者由于无放射污染之虑,酶联抗体可较长期地保存,应用较为方便。我们在制备抗胃癌细胞株MGC803单克隆抗体时,采用ELISA进行筛选及检测,对靶细胞的固相化,高质量酶联抗体的制备等环节进行了改进,获得较为理想的结果。     

普萘洛尔或血管紧张素Ⅱ结合胃癌单克隆抗体与丝裂霉素交联物导向治疗的实验研究

观察了普萘洛尔和血管紧张素Ⅱ(AT-Ⅱ)对胃癌单克隆抗体3H11和丝裂霉素(MMC)交联物导向治疗的增强作用。在荷瘤裸鼠体内观察,交联物的抑瘤率达50％,联合应用普萘洛尔或AT-Ⅱ,可使抑瘤率分别提高到79％和60％。从~(131)Ⅰ-3H11,在荷瘤裸鼠体内的生物学分布实验中也看到,普萘洛尔或AT-Ⅱ均能提高肿瘤组织对~(131)Ⅰ-3H11的摄取量。此结果表明,血管活性物质确可通过改善肿瘤血管的血流灌注,增加肿瘤内交联物含量,增强导向治疗的疗效。     

中国癌症控制策略研究报告

据报道2000年全球癌症发病及死亡人数分别为1010万和620万。危害较大的癌症依次为肺癌、乳腺癌、结直肠癌、胃癌及肝癌,发达国家癌症的发病及死亡率明显高于发展中国家。全球癌症发病及死亡率继续增长的趋势预示,癌症将成为新世纪人类的第一杀手,已构成对公共卫生的巨大挑战。20世纪90年代以来,美国癌症的发病及死亡率均开始下降,这主要归功于控制吸烟及早诊早治等有效的干预措施。     

THE SPECIFIC PHOTODYNAMIC EFFECTS OF MONOCLONAL ANTIBODIES CONJUGATED WITH HEMATOPORPHYRIN DERIVATIVE ON GASTRIC CANCER IN VITRO AND IN VIVO

Murine monoclonal antibody (MoAb) BB4.3, raised against the human gastric cancer cell line BGC823, was puriffied with Protein A-Sepharose CL-4B affinity chromatography and identified as IgG2a. It was then conjugated with a hematoporphyrin derivative (HPD) by using carbodiimide. The qualitative analysis of this conjugate showed that the amount of free HPD was negligible and there were no IgG aggregates among the conjugates. The conjugate retained both the antibody and photochemical activity of HPD.In vitro, the phototoxic effect of this HPD-BB4.3 conjugate on target cells was about 15 times higher than that of free HPD. The quality of selective photocytotoxicity was proven by the greater cytotoxi-city this conjugate showed than that of corresponding normal mouse IgG (NIgG) conjugated with HPD. It showed less cytotoxicity to colon cancer cell line B-80 (negative reaction to MoAb BB4.3) than to BGC825. Moreover, its cytotoxicity to BGC823 cells could be blocked specifically by excess BB4.3 antibody, but no