基于DSP-FPGA平台的ADCP高沙流量测量系统

基于TMS320C6748与Spartan-6平台完成声学多普勒流速剖面仪(Acoustic Doppler Current Profiler, ADCP)的设计,并实现高含沙量情况下的水流流量精确测量。数字信号处理模块(Digital Signal Processing, DSP)完成上位机通信、系统控制、现场可编程门阵列模块(Field Programmable Gate Array, FPGA)交互、外设数据接入、信号数据处理等功能,FPGA完成发射信号调制、接收信号采集等功能。利用测量船搭载ADCP高沙流量测量系统在黄河流域进行测量,ADCP接入全球定位系统(Global Positioning System, GPS)和测深仪数据,在高含沙量水域进行断面流量测试,验证系统的可行性及测量精度。结果显示,在含沙量达到15.5 kg·m-3的水文环境下,ADCP系统可正常稳定地完成测量作业,测量得到的流量相对误差为1.01%,满足高精度流量测量系统的要求。     

Enhanced antibody-dependent cellular phagocytosis by chimeric monoclonal antibodies with tandemly repeated Fc domains

We previously reported that chimeric monoclonal antibodies (mAbs) with tandemly repeated Fc domains, which were developed by introducing tandem repeats of Fc domains downstream of 2 Fab domains, augmented binding avidities for all Fcγ receptors, resulting in enhanced antibody (Ab)-dependent cellular cytotoxicity. Here we investigated regarding Ab-dependent cellular phagocytosis (ADCP) mediated by these chimeric mAbs, which is considered one of the most important mechanisms that kills tumor cells, using two-color flow cytometric methods. ADCP mediated by T3-Ab, a chimeric mAb with 3 tandemly repeated Fc domains, was 5 times more potent than that by native anti-CD20 M-Ab (M-Ab hereafter). Furthermore, T3-Ab-mediated ADCP was resistant to competitive inhibition by intravenous Ig (IVIG), although M-Ab-mediated ADCP decreased in the presence of IVIG. An Fcγ receptor-blocking study demonstrated that T3-Ab mediated ADCP via both FcγRIA and FcγRIIA, whereas M-Ab mediated ADCP exclusively via FcγRIA. These results suggest that chimeric mAbs with tandemly repeated Fc domains enhance ADCP as well as ADCC, and that Fc multimerization may significantly enhance the efficacy of therapeutic Abs.