紫草油脂肪酸尿素包合后的组成分析

通过对紫草油脂肪酸尿素包合后的液相及固相中脂肪酸组成的分析，确定尿素包合法分离提纯含两种以上多烯脂肪酸的效果。实验采用色－质联用仪，分析测试了紫草油脂肪酸经尿素包合后固相和液相中脂肪酸的组成。结果表明，尿素包合法可以富集多烯不饱和脂肪酸，使液相中亚油酸、亚麻酸、十八碳四烯酸三种多烯脂肪酸的质量分数高达94.6%，单烯和饱和脂肪酸的质量分数降为5.4%，但与尿素形成固相包合物的脂肪酸中亚油酸、亚麻酸、十八碳四烯酸三种多烯脂肪酸的质量分数仍达66.4%。因此，采用一次尿素包合法分离提纯含两种以上的多烯脂肪酸的植物油效果不很理想，需选用与柱层析相结合的手段做进一步的分离。     

中华绒螯蟹苗种生产中副溶血性弧菌病的诊断与防治

为了研究中华绒鳌蟹副溶血性弧菌病的症状、诊断及防治方法，从发病蟹苗体上分离出病原体，提纯培养后经全自动微生物分析仪分析鉴定，再用三糖铁培养基培养确定为副溶血性孤菌，然后进行药酶试验。试验表明，其对氧哌嗪青霉素和呋喃西林高度敏感，对庆大霉素、新霉素等中度敏感，对复方新诺明、青霉素G等轻度敏感，而对链霉素、土霉素等则不敏感。在此基础上提出采用生态学方法进行预防，并采用中草药及抗生素进行治疗的防治措施。     

Optimization of extraction and determination of emodin from Polygonum cuspidatum Sieb. et Zucc. products by HPLC-DAD

A uniform experimental design procedure was used to investigate the effects of some operating parameters on the extraction of emodin from Polygonum cuspidatum Sieb. et Zucc. products. Variables tested were volume ratio of material to solvent, size of material, extraction time and temperature and composition of extraction solvent （mixtures of acetone-water）. Each variable was tested at seven levels; 7 experiments were performed in random order. Analyses of the extracts were performed by high-performance liquid chromatography with diode array detection（HPLC-DAD）. Analytical responses were processed by using a forward regression analysis, in order to find polynomial function describing the relationship between variables and responses. For all the analytes the experimental conditions for providing the highest extraction yield inside the experimental domain considered were found. Finally, a simple, rapid and accurate analytical method was developed for the determination of emodin by high performance liquid chromatography. The separation is achieved within 25 rain on an ODS column using methanol and water as gradient mobiles. Emodin can be quantified by using external standard method detecting at 436 nm. Good linearity is obtained with correlation coefficient exceeding 0.9986 and the detection limit and the quantification limit are 1.53 and 3.23 mg/L respectively. This method shows good reproducibility for the quantification of the emodin with intra-day and inter-day relative standard deviation less than 2.3% and 5.6% respectively. Under optimized extraction conditions, the recovery of the standard is 96.5%. The validated method is successfully applied to quantify the emodin in seven Polygonum cuspidatum sieb. Et zucc. products, which provided an idea for the pre-treatment of determination of active compounds in traditional Chinese medicines.     

中草药化妆品

简介了常用于化妆品的六种中草药的有效成分、护肤护发作用、提制方法和配方实例。     

鬼针草微量元素的测定

采用光度法测定了中草药鬼针草中向量元素Ｃｕ、Ｆｅ、Ｚｎ、Ｍｎ、Ｎｉ、Ｃｒ的含量。     

我国中草药化妆品实现国际标准化初探

分析了中国化妆品行业的现状和问题，指出应发挥我国的优势和特色，大力发展中草药化妆品，提出了促进中草药化妆品国际标准化的观点，并探讨了化妆品行业实现这一目标的途径。     

医药、农药及中间体

“中草药 卷烟”中式卷烟发展跃升新平台；大环内酯类原料药硫氰酸红霉素出口居首；国内乙二醇市场低迷；石家庄制药集团维生公司维C国内销量成倍增长；赛诺菲思诺思缓释剂型07年将在我国上市；邻(对)硝基苯烷醚新工艺获国家专利。     

HPLC法测定陆英中乌索酸和齐墩果酸含量

用SymmetryC18色谱柱，流动相甲醇-水(88：12)，检测波长220nm，流速0.6mL／min，柱温25℃，测定陆英药材中乌索酸和齐墩果酸含量。试验结果表明：峰面积RSD(相对标准差值)均小于2．5％，乌索酸和齐墩果酸平均回收率分别为98．32％(RSD为2．12％)和97.92％(RSD为1．69％)。本法可同时测定乌索酸和齐墩果酸含量，具有准确、灵敏、数据可靠的优点，可用于陆英药材质量控制。