Enantioseparation of Novel Amino Analogs of Indole Phytoalexins on Macrocyclic Glycopeptide-Based Chiral Stationary Phase

Direct chiral separation of the enantiomers of spirobrassinin, 1-methoxyspirobrassinin and ten novel cis- and trans-diastereoisomers of 2-amino analogs of indole phytoalexin 1-methoxyspirobrassinol methyl ether on macrocyclic glycopeptide-based chiral stationary phase (CSP) with teicoplanin (Chirobiotic T) was studied. Normal phase eluents containing n-hexane with modifiers ethanol and 2-propanol were used. The effects of mobile phase composition, structure of the analytes and temperature were investigated. Chiral resolution on teicoplanin CSP was achieved only in the case of trans-diastereoisomers. The van??t Hoff plots were found to show linear behavior in all cases. It was found that studied normal phase enantioseparations were enthalpy driven. The elution order of the enantiomers was determined in some cases.     

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目的分析1000例儿童肾活检的成功率及并发症。方法收集2005-01-01—2012-1-31在成都市妇女儿童中心医院儿童肾内科就诊的肾脏病患儿1000例,在B超引导下经皮穿刺活检,分析取材长度、活检针数与成功率及并发症的关系。结果 962例获得足够肾组织,平均肾小球(24.1±12.3)个(5～53个),满足光镜、电镜、免疫荧光检查的需要,并做出了完整的病理诊断;10例虽然穿刺成功,但全为髓质组织,没有肾小球;28例肾小球数少于5个,未做出病理诊断;总成功率96.2%。其主要并发症:患者术后出现并发症17%,其中一过性肉眼血尿85例,占8.5%(85/1000例),部分患儿有腰部不适75例,占7.5%(75/1000例),肾周血肿10例,占1%,无感染、休克、动静脉瘘等严重并发症发生。结论超声引导下儿童肾活检成功率高、并发症少,是安全可靠的。