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Tao Zhang Guangquan Zhang Wenbo Yang Hongze Chen Jisheng Hu Zhongjie Zhao Chundong Cheng Guanqun Li Yu Xie Yilong Li Rui Kong Yongwei Wang Gang Wang Hua Chen Xue-Wei Bai Shangha Pan Bei Sun Le Li 《Cell death & disease》2021,12(11)
Chronic pancreatitis (CP) is described as progressive inflammatory fibrosis of pancreas, accompanied with irreversible impaired endocrine and exocrine insufficiency. Pancreatic stellate cells (PSCs) are widely distributed in the stroma of the pancreas and PSCs activation has been shown as one of the leading causes for pancreatic fibrosis. Our previous study has revealed that autophagy is dramatically activated in CP tissues, which facilitates PSCs activation and pancreatic fibrosis. Long non-coding RNAs (LncRNAs) have been recognized as crucial regulators for fibrosis-related diseases. LncRNAs interact with RNA binding protein or construct competitive endogenous RNA (ceRNA) hypothesis which elicited the fibrotic processes. Until now, the effects of lncRNAs on PSCs activation and pancreatic fibrosis have not been clearly explored. In this study, a novel lncRNA named Lnc-PFAR was found highly expressed in mouse and human CP tissues. Our data revealed that Lnc-PFAR facilitates PSCs activation and pancreatic fibrosis via RB1CC1-induced autophagy. Lnc-PFAR reduces miR-141 expression by suppressing pre-miR-141 maturation, which eventually upregulates the RB1CC1 and fibrosis-related indicators expression. Meanwhile, Lnc-PFAR enhanced PSCs activation and pancreatic fibrosis through trigging autophagy. Our study interrogates a novel lncRNA-induced mechanism in promoting the development of pancreatic fibrosis, and Lnc-PFAR is suggested to be a prospective therapeutic target in clinical scenarios.Subject terms: RNAi, Diagnostic markers, Chronic pancreatitis 相似文献
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Zhonglou SUN Pablo OROZCO-TERWENGEL Guotao CHEN Ruolei SUN Lu SUN Hui WANG Wenbo SHI Baowei ZHANG 《动物学报(英文版)》2021,67(4):361-370
Climate fluctuations in the past and in the future are likely to result in population expansions,shifts,or the contraction of the ecological niche of many speci... 相似文献
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Preparation, characterization and properties of superabsorbent nanocomposites based on natural guar gum and modified rectorite 总被引:1,自引:0,他引:1
Novel guar gum-g-poly(sodium acrylate)/rectorite (GG-g-PNaA/REC) superabsorbent nanocomposites were prepared in aqueous solution using guar gum (GG), partially neutralized acrylic acid (NaA), acidified rectorite (H+-REC) and organified rectorite (CTA+-REC) by cetyltrimethylammonium bromide (CTAB) as raw materials, ammonium persulfate (APS) as initiator and N,N′-methylenebisacrylamide (MBA) as crosslinker. FTIR spectra confirmed that NaA had been grafted onto GG chains and the OH groups of REC participated in polymerization reaction. Exfoliated nanocomposite was obtained for H+-REC and intercalated structure was formed for CTA+-REC as shown by XRD results. SEM observations show REC has been uniformly dispersed in polymeric matrix. Effects of HCl concentration, organification degree of CTA+-REC and content of REC on swelling capabilities were investigated and the swelling kinetics of nanocomposites was evaluated. Results indicate that modifying REC by acidification and organification can improve swelling properties of the resultant nanocomposites, and GG-g-PNaA/CTA+-REC exhibited higher swelling capability and swelling rate contrast to GG-g-PNaA/H+-REC. 相似文献
15.
Bin Liang Yu Zheng Juan Wang Wenbo Zhang Ying Fu Wenbin Kai Yandan Xu Bing Yuan Qian Li Ping Leng 《The Plant journal : for cell and molecular biology》2020,102(6):1220-1233
β‐Glucosidases (BG) are present in many plant tissues. Among these, abscisic acid (ABA) β‐glucosidases are thought to take part in the adjustment of cellular ABA levels, however the role of ABA‐BG in fruits is still unclear. In this study, through RNA‐seq analysis of persimmon fruit, 10 full‐length DkBG genes were isolated and were all found to be expressed. In particular, DkBG1 was highly expressed in persimmon fruits with a maximum expression 95 days after full bloom (DAFD). We verified that, in vitro, DkBG1 protein can hydrolyze ABA‐glucose ester (ABA‐GE) to release free ABA. Compared with wild‐type, tomato plants that overexpressed DkBG1 significantly upregulated the expression of ABA receptor PYL3/7 genes and showed typical symptoms of ABA hypersensitivity in fruits. DkBG1 overexpression (DkBG1‐OE) accelerated fruit ripening onset by 3–4 days by increasing ABA levels at the pre‐breaker stage and induced early ethylene release compared with wild‐type fruits. DkBG1‐OE altered the expression of ripening regulator NON‐RIPENING (NOR) and its target genes; this in turn altered fruit quality traits such as coloration. Our results demonstrated that DkBG1 plays an important role in fruit ripening and quality by adjusting ABA levels via hydrolysis of ABA‐GE. 相似文献
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Water level variation is considered as a main controlling environment factor affecting the growth of aquatic vegetation in Poyang Lake, the largest freshwater lake in China. It is significant to study the influences of water level change on aquatic vegetation under various hydrological conditions. Taking the free connected sub-lake Bang Lake and partially controlled sub-lake Dahuchi Lake of Poyang Lake as research objects and based on the cloud computing platform of remote sensing of Google Earth Engine (GEE), the pixel binary model method was used to estimate the aquatic vegetation cover from 2000 to 2019, and the spatial and temporal variation characteristics were discovered first. Meanwhile, the combined method of Sen + M-K (Theil–Sen Median method and Mann-Kendall trend test) was conducted to simulate the trends of water fluctuation. Second, the hydrological parameters indicating water level fluctuation were established, and the relationship between these parameters and the area covered by aquatic vegetation in different sub-lakes was explored. The results were as follows. (1) The aquatic vegetation cover of the freely connected sub-lake Bang Lake was more susceptible to water level changes, while the partially controlled sub-lake Dahuchi Lake was relatively stable underwater level change. (2) The aquatic vegetation was patchily and sporadically distributed in years with low vegetation cover, while in the years with high vegetation cover, it was distributed in a ringlike pattern, spreading from the centre of the lake to the shore. (3) The aquatic vegetation cover of the freely connected sub-lake Bang Lake was more likely to be influenced by the water level fluctuation (WLF), while the aquatic vegetation cover of the partially controlled sub-lake Dahuchi Lake was more likely to be influenced by the flooding duration of 17 m water level (L17). The flooding duration of 19 m water level (L19) had a strong negative correlation with the predicted aquatic vegetation cover of both Bang Lake and Dahuchi Lake. It suggests that there was a certain degree of time lag in the effect of water level changes on the free connected sub-lakes, but there was no obvious time lag on the partially controlled sub-lakes. (4) The aquatic vegetation of the freely connected sub-lake Bang Lake were dominated by stabilization and slight improvement, while aquatic vegetation in the partially controlled sub-lake Dahuchi Lake were dominated by stabilization and significant degradation. This study can help to further understand the vegetation changes in hydro-ecological system with different hydrological connectivity and will provide a reference for lake management and conservation. 相似文献
19.
Wenbo Xu;Shengchun Li;Ralph Bock;Jiang Zhang; 《Plant biotechnology journal》2024,22(4):960-969
Inducible expression systems can overcome the trade-off between high-level transgene expression and its pleiotropic effects on plant growth. In addition, they can facilitate the expression of biochemical pathways that produce toxic metabolites. Although a few inducible expression systems for the control of transgene expression in plastids have been developed, they all depend on chemical inducers and/or nuclear transgenes. Here we report a temperature-inducible expression system for plastids that is based on the bacteriophage λ leftward and rightward promoters (pL/pR) and the temperature-sensitive repressor cI857. We show that the expression of green fluorescent protein (GFP) in plastids can be efficiently repressed by cI857 under normal growth conditions, and becomes induced over time upon exposure to elevated temperatures in a light-dependent process. We further demonstrate that by introducing into plastids an expression system based on the bacteriophage T7 RNA polymerase, the temperature-dependent accumulation of GFP increased further and was ~24 times higher than expression driven by the pL/pR promoter alone, reaching ~0.48% of the total soluble protein. In conclusion, our heat-inducible expression system provides a new tool for the external control of plastid (trans) gene expression that is cost-effective and does not depend on chemical inducers. 相似文献
20.
Hang Zheng Hong Li Wenbo Tan Chunlin Xu Lijuan Jia Dandan Wang Zhuanjian Li Gunrong Sun Xiangtao Kang Fengbin Yan Xiaojun Liu 《Journal of genetics》2018,97(1):145-155
The cathepsin E-A-like, also known as ‘similar to nothepsin’, is a new member of the aspartic protease family, which may take part in processing of egg yolk macromolecules, due to it was identified in the chicken egg-yolk. Previously, studies have suggested that the expression of cathepsin E-A-like increased gradually during sexual maturation of pullets, but the exact regulation mechanism is poorly understood. In this study, to gain insight into the function and regulation mechanism of the gene in egg-laying hen, we cloned the cathepsin E-A-like gene and evaluated its evolutionary origin by using both phylogenetic and syntenic methods. The mode of the gene expression regulation was analysed through stimulating juvenile hens with \(17\upbeta \)-estradiol and chicken embryo hepatocytes with \(17\upbeta \)-estradiol combined with oestrogen receptor antagonists including MPP, ICI 182,780 and tamoxifen. Our results showed that cathepsin E-A-like was an orthologoues gene with nothepsin, which is present in birds but not in mammals. The expression of cathepsin E-A-like significantly increased in a dose-dependent manner after the juvenile hens were treated with \(17\upbeta \)-estradiol (\(P~<~0.05\)). Compared with the \(17\upbeta \)-estradiol treatment group, the expression of cathepsin E-A-like was not significantly changed when the hepatocytes were treated with \(17\upbeta \)-estradiol combined with MPP (\(P~<~0.05\)). In contrast, compared with the \(17\upbeta \)-estradiol combined with MPP treatment group, the expression of cathepsin E-A-like was significantly downregulated when the hepatocytes were treated with \(17\upbeta \)-estradiol combined with tamoxifen or ICI 182,780 (\(P~<~0.05\)). These results demonstrated that cathepsin E-A-like shared the same evolutionary origin with nothepsin. The expression of cathepsin E-A-like was regulated by oestrogen, and the regulative effect was predominantly mediated through ER-\(\upbeta \) in liver of chicken. 相似文献