基因活化剂对紫色肉甘薯藤蔓生长的影响

基因活化剂使用浓度(A)设3个水平:375×(A1),750×(A2),1500×(A3),处理时间(B)设3个水平:浸渍 种苗基部0.5h(B1),1h(B2),2h(B3),处理后培养在改良Hoagland培养基中,10d后测定藤蔓长度和鲜质量.结 果表明:A1,A2和A3的藤蔓长度分别比对照(32.1cm)提高17.60%,43.61%和12.06%,藤蔓鲜质量分别比对照 (142.69g)提高63.30%,109.06%和55.01%;B1,B2和B3的藤蔓长度分别比对照提高17.07%,36.39%和 19.81%,藤蔓鲜质量分别比对照提高67.05%,97.76%和62.55%,与对照差异均达极显著水平(p<0.01),不同 水平间差异极显著(p<0.01).A2B2为最佳水平组合,藤蔓长度比对照提高51.34%,鲜质量提高114%.用750倍 稀释液浸渍种苗基部1h,可显著促进紫色肉甘薯藤蔓的生长.     

Role of plasminogen activator and plasminogen activator inhibitor type-1 in luteolysis—a minireview

This minireview summarized our recent studies on the role of plasminogen activator (PA) and inhibitor type-1 (PAI-1) in luteolysis. We have demonstrated that (1) both tissue type and urokinase type plasminogen activators (tPA and uPA) and a plasminogen activator inhibitor type-1 (PAI-1) were present in the corpus luteum of rat and rhesus monkey; (2) decrease in progesterone production in corpus luteum was well correlated with a sharp increase in tPA (but not uPA) and PAI-1 secretion; (3) exogenous tPA decreased luteal progesterone synthesis while monoclonal antibodies increased progesterone production; (4) interferony inhibited luteal progesterone synthesis and stimulated tPA production while LH plus prolactin increased progesterone production and decreased tPA (hut not uPA) activity in cultured luteal cells; (5) increase in proteolysis in the corpus luteum was also correlated with decrease in progesterone production in mouse. These data suggest that local degradation of extracellular matrix controlled by plasminogen activator and inhibitor is involved in the processes of luteolysis.     

Interferon-β can induce the production of plasminogen activator by cultured human cancer cells

Summary Three cultured human cell lines, renal cancer cells (ACHN), bladder cancer cells (EJ), and fibroblasts transformed in culture by Co-60 gamma rays (KMST-6), when treated with interferon-, produced 1.5 to 4 times as much plasminogen activator as the untreated control cultures. This enhanced production of PA was inhibited by cycloheximide or actinomycin D.     

Effect of radical scavengers on TNFα-mediated activation of the uPA in cultured cells

Active oxygen, produced by cultured cells following stimulation with various growth factors, seems to be involved in signal transduction leading to cellular responses such as gene expression and growth modulation. In the present study, the intracellular oxidation state was measured in immortalized human endothelial cells (ECV304) after treatment with tumor necrosis factor (TNF), using a fluorescent dye and a laser-scanning confocal microscope. The intracellular oxidation state was increased 60 min after the addition of TNF, and this increase was abolished by a radical scavenger, N-acetylcysteine (NAC), which is also a precursor of glutathione, and by pyrrolidine dithiocarbamate (PDTC). TNF increased the steady state level of urokinase-type plasminogen activator (uPA), and NAC inhibited this increase at a dose that also inhibited the increase in the intracellular oxidation state. PDTC, on the other hand, did not affect the induction of the uPA gene by TNF. These results suggest that intracellular glutathione level rather than the oxidation state is necessary for the induction of the uPA gene by TNF.     

一种脂肪酶激活剂的性质的初步研究

在分析、比较了多种中草药提取物对脂肪酶的激活效率的基础上 ,作者用温和的生物工程手段 ,从中药紫花地丁中纯化得到高效的脂肪酶激活剂 ,当浓度达到 16 6 .6 7mg/ml,酶浓度为 40 mg/m l时 ,激活效率可达2 80 % .研究表明 ,此激活剂对脂酶具有竞争性与反竞争性的混合激活效应 ,并且该激活剂具有热稳定性 .在 p H =7.0的反应条件下 ,激活效率达到最高     

环保型表面清洗剂的研制

环保型硬表面清洗剂广泛应用于清洗工业性油污,本文主要介绍清洗剂的研制过程,并对该产品使用的效果加以说明。     

苯并噻二唑标样的制备及Bion 50%WG含量分析

从Bion 50%WG中提取有效成分制备了苯并噻二唑(BTH)标样,其结构经1H NMR谱I、R、元素分析和HPLC/MS的确证.利用制备的标样建立了Bion 50%WG的HPLC分析方法,用250 mm×4.6 mm不锈钢ODS 5μm球型硅胶填料色谱柱,甲醇(含0.6%的冰醋酸)/水(含0.6%的冰醋酸)=85/15(V/V)为流动相,1.0 mL.min-1的流速和254 nm的检测波长能较好地分离BTH与样本中的杂质.方法的平均添加回收率为99.91%,Bion 50%WG的平均含量为49.47%,变异系数为0.30%,线性相关系数平方(r2)为0.999 0.