上海地区市售生鲜肉中单核细胞增生李斯特菌和沙门氏菌的污染监测分析

目的 调查上海地区市售生鲜肉中单核细胞增生李斯特菌和沙门氏菌的污染情况。方法 2018年7月到2019年4月,从上海市88家农贸市场和42家超市抽样308件,其中鲜猪肉114件、整鸡92件、鲜牛肉102件。按食品安全国家标准分别进行单核细胞增生李斯特菌和沙门氏菌的检测,采用VITEK2全自动生化鉴定仪对疑似菌株进行鉴定确认,并对沙门氏菌分离株进行血清分型。结果 生鲜肉中单核细胞增生李斯特菌和沙门氏菌的检出率为分别为28.2%和39.6%,其中鲜牛肉（48.0%）中单核细胞增生李斯特菌的检出率显著高于猪肉（17.5%）和整鸡（19.6%）（P<0.001）,而猪肉（46.5%）和整鸡中（59.8%）沙门氏菌的检出率则显著高于牛肉（13.7%）（P<0.001）;农贸市场采集的鲜肉样品中单 核细胞增生李斯特菌（P=0.008）和沙门氏菌（P＜0.001）的污染率均显著高于超市;血清学试验结果显示122株沙门氏菌分布于21种不同血清型,其中Corvallis血清型（14.75%）流行率最高。结论 上海地区市售生鲜肉中存在较高的单核细胞增生李斯特菌和沙门氏菌的污染率,极易引发食源性疾病,建议政府监管部门加强对生鲜肉食品的监管。     

Comparison of Rice Flour Pasting Properties using Brabender Viscoamylograph and Rapid Visco Analyser for Evaluating Cooked Rice Texture

Pasting properties of ten Thai rice varieties, with amylose contents in the range of 16 to 33% and stored at 10, 30 and 40 °C for six months were compared using the Brabender Viscoamylograph (VAG) and the Rapid Visco Analyser (RVA) for evaluating the texture of cooked rice. Linear correlations between pasting temperature and viscosity parameters based on RVA showed a different pattern than those based on VAG with high negative correlation coefficients between the peak and breakdown viscosities. Both RVA and VAG indicated peak viscosity to be positively correlated with breakdown viscosity, and negatively with the setback viscosity. Setback and consistency viscosities correlated with each other in both instruments but indicating a negative correlation with breakdown viscosity in VAG only. Pasting temperature determined by VAG and RVA did not show good correlations for rice sample stored at different temperatures neither for individual nor for combined data. Only peak, breakdown and setback viscosities showed significant correlations in the overall data (r > 0.496). The VAG measurements performed better than the RVA in the development of predictive models for evaluating the hardness and adhesiveness of cooked rice based on instrumental texture profile analysis. The springiness and cohesiveness of cooked rice could not be estimated from the pasting properties of rice flour determined by both VAG and RVA. Results showed that both VAG and RVA could be used for evaluating the texture of cooked rice despite the differences in the measurements of the pasting properties of milled rice flours.