不同时机口服美林拮抗白细胞介素-2发热反应的临床观察

[目的]探讨用关林减轻白细胞介素-2（IL-2）发热反应的用药时机。[方法]9例恶性实体肿瘤患儿共28个疗程140次用IL-2进行免疫治疗，按使用美林时机分组，对照组40次，按传统的治疗护理方案，即在静脉输注后体温达39℃时遵医嘱口服美林；实验1组50次，在静脉输注1．5h～2．0h时遵医嘱口服关林；实验2组50次在静脉输注结束时体温未达高峰时遵医嘱口服美林，观察降温疗效。[结果]对照组中有30次在静脉输注完后1h～2h达体温峰值。6次体温峰值达39℃以上；实验1组体温峰值降低的次数明显高于实验2组和对照组（P〈0．05），总有效率也高于实验2组和对照组（P〈0．05）。而实验2组和对照组无显著差异（P〉0.05）。[结论]在静脉输注白细胞介素-21．5h～2．0h口服美林，能显著提高对抗IL-2引起机体发热反应的有效率，提高病人在用药过程中的耐受力，减轻病人痛苦。     

实用新型下肢悬吊架的研制与临床应用

目的研制一种制作简单且方便实用的新型下肢悬吊架。方法将268例四肢手术患者随机分为2组,实验组采用新型下肢悬吊架进行消毒,对照组按常规方法消毒,2组患者具体消毒方法相同,观察2组消毒时间、患者感染数、医生满意度、手术人员及患者的污染情况等。结果实验组消毒时间短,患者感染率低,医生满意度高。结论新型实用下肢悬吊架提高了患者舒适度,减少了医护人员的劳动量,降低了手术感染率,提高了下肢消毒时患者体位的稳定性。     

纳米羟基磷灰石悬浮液稳定性能的研究

为了得到稳定分散的纳米羟基磷灰石水悬浮液,以羧甲基纤维素钠（CMCNa）作分散剂,采用吸光率实验考察了羧甲基纤维素钠的用量及超声分散时间对纳米羟基磷灰石水悬浮液稳定性的影响,并对其分散机理进行了初步的探讨。结果表明：CMCNa作分散剂时,能有效地分散纳米羟基磷灰石粒子,得到均匀、稳定的纳米羟基磷灰石水悬浮液体系;当CMCNa用量为0.35wt%、超声时间为150s时分散效果最佳;CMCNa分散后,体系的Zeta电位的绝对值提高了69.82mV;CMCNa对纳米HAP悬浮液的稳定分散作用主要是通过粒子间的静电作用和空间位阻来实现的。由此可见,CMCNa对HAP纳米粒子具有较好的分散效果,是制备高分散稳定HAP的理想分散剂。     

10％杀虫悬浮剂现场防治蚊虫的效果观察

目的探讨流行性乙型脑炎传播蚊媒的防治方法，为指导灭蚊提供科学依据。方法现场实验研究。结果在南通地区使用大功达10％杀虫悬浮剂进行滞留喷洒对蚊虫具有速杀作用和一定的持效。处理后1h和24h蚊虫密度下降率为92．7％和82％，3d后仍能达到38．8％。结论氯氰菊酯滞留喷洒对蚊虫具有较好的防治效果。     

喜树胚轴细胞悬浮培养的优化和喜树碱的积累

目的优化喜树胚轴细胞悬浮培养体系生产喜树碱。方法将由喜树种子下胚轴诱导的愈伤组织进行悬浮培养,研究了基本培养基、激素和培养条件对培养物生长的影响,测定了培养细胞生长和喜树碱积累的动态变化。结果悬浮培养细胞在M S培养基中细胞生长良好,细胞分散性和喜树碱积累优于在其他培养基中。最佳激素组合为2,4-D 0.2 m g/L NAA 0.5 m g/L 6-BA 0.5 m g/L,细胞继代周期以12 d左右为宜,悬浮细胞适合在22~26℃,120 r/m in下生长。悬浮培养细胞在第4~20天为对数生长期,细胞倍增时间为t(d)=3.92-d 1,细胞干重至第20天达到最大,为27.44 g/L。在细胞对数生长期内,喜树碱积累与细胞生长呈线形正相关性,第20天喜树碱质量分数达到最大值9.319×10-5,喜树碱产量为2.56 m g/L。结论M S基本培养基适合喜树胚轴细胞悬浮培养,细胞生长前20天内,培养体系中喜树碱积累与细胞生长呈正线形相关性。     

5-HT7 receptor inhibition and inactivation induce antidepressantlike behavior and sleep pattern.

BACKGROUND: The 5-hydroxytryptamine7 receptor (5-HT7) is implicated in circadian rhythm phase resetting, and 5-HT7 receptor-selective antagonists alter rapid eye movement (REM) sleep parameters in a pattern opposite from those in patients with clinical depression. METHODS: As sleep, circadian rhythm, and mood regulation are related, we examined 5-HT7 receptor knockout mice in two behavioral models of depression. The forced swim and tail suspension tests are highly predictive for antidepressant drug activity. RESULTS: Unmedicated 5-HT7-/- mice showed decreased immobility in both tests, consistent with an antidepressantlike behavior. The selective 5-HT7 receptor antagonist SB-269970 also decreased immobility. The selective serotonin reuptake inhibitor citalopram, a widely used antidepressant, decreased immobility in both 5-HT7+/+ and 5-HT7-/- mice in the tail suspension test, suggesting that it utilizes an independent mechanism. The 5-HT7-/- mice spent less time in and had less frequent episodes of REM sleep, also consistent with an antidepressantlike state. CONCLUSIONS: The 5-HT7 receptor might have a role in mood disorders and antagonists might have therapeutic value as antidepressants.     

Antidepressant-like effect of ethanol extract from Paeonia lactiflora in mice

The present study investigated the antidepressant effect of ethanol extract of Paeonia lactiflora (EPL) in mice using forced swim test, tail suspension test, open-field test and reserpine test. Our results showed that intragastric administration of EPL at the doses of 250 and 500 mg/kg for seven days significantly reduced the duration of immobility in both forced swim test and tail suspension test. EPL at the dose of 500 mg/kg was as effective as the positive control (chlorimipramine, 20 mg/kg) in these tests. However, these treatments did not affect the number of crossing and rearing in the open-field test. Treating mice with EPL at the doses of 250 and 500 mg/kg significantly antagonized reserpine-induced ptosis and hypothermia. However, at the dose of 125 mg/kg, EPL antagonized only the hypothermia but not ptosis induced by reserpine. The results clearly demonstrated the antidepressant effect of Paeonia lactiflora in animal models of depression. The action of Paeonia lactiflora may be mediated via the central monoaminergic neurotransmitter system.     

Relationship of the uterosacral ligament to the sacral plexus and to the pudendal nerve

We describe the anatomy of the uterosacral ligament with respect to the sacral plexus. In six adult female embalmed cadavers, we identified the uterosacral ligament and its lateral nerve relations. Using the ischial spine as the starting point and measuring along the axis of the uterosacral ligament, we noted that the S1 trunk of the sacral plexus passes under the ligament 3.9 cm [95% confidence interval (CI), 2.1–5.8 cm] superior to the ischial spine. The S2 trunk passes under the ligament at 2.6 cm (95% CI; 1.5, 3.6 cm), the S3 trunk passes under the ligament at 1.5 cm (95% CI; 0.7, 2.4 cm), and the S4 trunk passes under the ligament at 0.9 cm (95% CI; 0.3, 1.5 cm) superior to the ischial spine. The pudendal nerve forms lateral to the uterosacral ligament. Our data demonstrate that the S1–S4 trunks of the sacral plexus, not the pudendal nerve, are vulnerable to injury during uterosacral ligament suspension.     

苯乙烯—甲基丙烯酸共聚物的合成,结构与性能研究

采用悬浮聚合法合成了苯乙烯－甲基丙烯酸共聚物（ＳＭＡＡ），得率为９２％左右。由Ｋｅｌｅｎ－Ｔｕｄｏｓ法求得苯乙烯（Ｓｔ）与甲基丙烯酸（ＭＡＡ）在８０℃进行共聚反应的竞聚率为γ＿（Ｓｔ）＝０．３０５，γ＿（ＭＡＡ）＝０．６００。通过正交试验，确定了最佳配方。经ＦＴ－ＩＲ、ＤＳＣ、元素分析、化学滴定、ＧＰＣ等表征，证实了所合成的产物为透明的苯乙烯－甲基丙烯酸无规共聚物。研究表明，ＳＭＡＡ的耐热性比ＰＳ好得多，其玻璃化温度随共聚物中ＭＡＡ含量增加而增大，活化能为１６６．３ｋＪ／ｍｏｌ。共聚物的加工性能良好，力学性能比ＰＳ有了明显提高。