Antioxidative constituents fromPaeonia lactiflora

The ethanol extract of the peony root (Paeonia Lactiflora Pall, Paeoniaceae) as well as its major active components including gallic acid and methyl gallate were evaluated for their protective effects against free radical generation and lipid peroxidation. In addition, the protective effects against hydrogen peroxide-induced oxidative DNA damage in a mammalian cell line were examined. The ethanol extracts of the peony root (PREs) and its active constituents, gallic acid and methyl gallate, exhibited a significant free radical scavenging effect against 1,1-diphenyl-2-picryl hydrazine (DPPH) radical generation and had an inhibitory effect on lipid peroxidation, as measured by the level of malondialdehyde (MDA) formation. The PREs did not have any pro-oxidant effect. They strongly inhibited the hydrogen peroxide-induced DNA damage from NIH/3T3 fibroblasts, as assessed by single cell gel electrophoresis. Furthermore, the oral administration of 50% PRE (50% ethanol extract of peony root), gallic acid and methyl gallate potently inhibited the formation of micronucleated reticulocytes (MNRET) in the mouse peripheral blood induced by a KBrO3 treatment in vivo. Therefore, PREs containing gallic acid and methyl gallate may be a useful antigenotoxic antioxidant by scavenging free radicals, inhibiting lipid peroxidation and protecting against oxidative DNA damage without exhibiting any pro-oxidant effect.     

A New antioxidant monoterpene glycoside, α-benzoyloxypaeoniflorin fromPaeonia suffruticosa

Alpha-benzoyloxypaeoniflorin (1), a new antioxidant monoterpene alpha-glycoside anomer was isolated from Paeonia suffruticosa along with known compounds, beta-benzoyloxypaeoniflorin (2), paeonolide, paeoniflorin and mudanpioside H. The structure of 1 has been determined by comparing spectral data with those of beta-benzoyloxypaeoniflorin (2). Compound 1 exhibited moderately potent radical scavenging activity on DPPH radical.     

Protective Effects for Hepalocytes of Salviae miltiorrhiza and Paeonia Iactiflora in Experimental Liver Damage

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六味地黄丸中丹皮的质量研究

本文以不同种丹皮为对照,对六味地黄丸的原料药丹皮进行了鉴定;用无水乙醇提取、薄层扫描法测定了丹皮药材及六味地黄丸中丹皮酚的含量。本法与水蒸汽蒸馏-紫外分光光度法,醇浸出-紫外分光光度法作了比较,排除了杂质干扰,结果准确可靠。     

Protective effects for hepatocytes ofSalviae miltiorrhiza andPaeonia lactiflora in experimental liver damage

The protective effects for hepatocytes ofSalviae miltiorrhiza andPaeonia lactiflora in experimental rat models of liver damage were observed. Pathological changes in light and electron micro-scopies and plasma fibronectin level alterations of liver injury in rats, revealed thatSalviae miltiorrhiza could induce the increase of plasma fibronectin level in rats, thus improved phagocytosis of the reticuloendothelial system and opsonin activity, preventing the liver from immunologic damage, protecting hepatocytes and promoting regeneration. Similar but weaker actions were observed inPaeoniae lactiflora.     

不同浸润时间对白芍饮片中芍药苷含量影响的研究

目的优选白芍切片的最佳浸泡时间。方法采用高效液相色谱(HPLC)测定不同浸润时间的白芍饮片中芍药苷含量。色谱条件D iamonsil C18色谱柱(250 mm×4.6 mm,5μm),流动相:乙腈-水(17∶83),流速1.0 m l.m in-1,检测波长230 nm。结果浸润12 h切片的白芍,其饮片中所含的芍药苷高于浸润24 h的白芍。结论白芍切片以浸润12 h比较适宜。     

HATR-FTIR结合主成分分析应用于赤芍的真伪鉴别

 目的建立中药材赤芍的质量分析方法。方法采用水平衰减全反射傅里叶变换红外光谱法(HATR-FTIR)测定了赤芍及其伪品的FTIR,并结合主成分分析法进行排序比较了正伪品之间的差异程度。结果发现基于傅里叶变换红外光谱的主成分分析在反映同属不同种及同种不同产地植物化学组成差异程度上具有应用价值。结论采用FTIR直接测定法并结合主成分分析法应用于赤芍的质量分析,简便、快速、准确,而且不需制备样品。     

归芍止痛汤治疗原发性痛经84例疗效观察

目的观察归芍止痛汤治疗原发性痛经的临床疗效。方法将符合条件的166例患者随机分为治疗组84例,对照组82例。治疗组给予归芍止痛汤治疗,对照组给予消炎痛治疗。结果治疗组治愈率65.5%,总有效率95.2%;对照组治愈率26.8%,总有效率70.7%。结论归芍止痛汤治疗原发性痛经疗效确切。     

HPLC法测定芍甘合剂中芍药苷及甘草苷含量

目的：建立芍甘合剂中芍药苷、甘草苷的含量测定方法。方法：通过高效液相色谱法，测定合剂中2种有效成分的含量。色谱柱：ZORBOX-ODS（150mm×4．6mm，5um）；流动相A为乙腈．B为三氟乙酸（0．25‰），比例为13：87；流速：1ml·min^-1；测定波长：230hm；柱温：25℃．结果：测得合剂中芍药普、甘草苷的含量。结论：本法简便、快捷、重现性好。     

杭白芍九种主要成分的高效液相色谱定量分析及提取工艺研究

目的: 建立同时测定杭白芍9种主要成分没食子酸、羟基芍药苷、儿茶素、芍药内酯苷、芍药苷、五没食子酰葡萄糖、苯甲酸、苯甲酰芍药苷和丹皮酚的高效液相色谱法（HPLC）,优化杭白芍的提取工艺。方法: HPLC检测采用0.1%磷酸水溶液-乙腈为流动相线性梯度洗脱,流速为0.8 mL/min,柱温为30℃,检测波长为230 nm。采用超声提取法,甲醇和乙醇为提取溶剂,选用L₉（3⁴）表分别设计三因素三水平的正交试验,考察溶剂浓度、液料比和提取时间对杭白芍9种主要成分总含量的影响。结果: 所建立的HPLC法具有较高的专属性、灵敏度和准确性,可用于同时定量分析杭白芍9种主要成分;确定了杭白芍9种主要成分的最佳提取工艺,即采用70%乙醇为提取溶剂,液料比为200 mL/g,超声提取时间为30 min。结论: 建立了同时测定杭白芍9种主要成分的HPLC法及其最佳工艺条件。