赤芍总苷静脉给药抗血栓形成作用研究

目的:研究赤芍总苷(TSP)静脉给药时抗凝和抗血栓形成作用.方法:TSP按18.8,37.5,75mg/kg三种剂量尾静脉注射,测定其对于小鼠凝血时间、复合诱导剂致肺栓塞的死亡率和角叉菜胶致尾血栓的长度比值及电刺激大鼠动脉血栓形成时间的影响.结果:TSP能显著延长小鼠凝血时间,降低肺栓塞死亡率,缩短尾血栓的长度比值,延长动脉血栓形成时间,0.375%浓度下未见溶血反应.结论:TSP静脉给药时具有显著抗凝和抗血栓形成作用.     

黄芪总皂苷与赤芍总苷协同抗血栓作用

运用肺栓塞、动脉血栓形成、脑血栓形成三种实验性血栓形成模型 ,观察黄芪总皂苷 (AS)和赤芍总苷 (TSP)以各种剂量配伍时的作用 ;结果AS和TSP均具有不同程度抗各种实验性血栓形成作用或趋势 ,配伍后作用更佳 ,抗凝作用则源于赤芍总苷 ,应用二因素五水平均匀设计方法观察到AS 5 0mg kg和TSP 15 0mg kg配伍时抗动脉血栓形成作用最佳 ,该剂量下两药配伍对于大鼠脑血栓有相加的保护作用。表明AS和TSP有协同抗血栓形成作用。     

Three new monoterpene glycosides from the roots of Paeonia lactiflora

Three new monoterpene glycosides, 2′-O-benzoylpaeoniflorin, albiflorin R₂, and albiflorin R₃ (1–3) were isolated from the roots of Paeonia lactiflora. Their structures were elucidated on the basis of spectroscopic means including one- and two-dimensional NMR experiments.     

HPLC 法测定赤芍在不同土壤肥力中芍药苷的含量

目的:测定赤芍在不同土壤肥力条件中芍药苷的含量。方法:采用高效液相色谱法,Spherisorb 10 ODS1(4.6 mm×250 mm,10μm),以甲醇∶0.05%mol/L磷酸二氢钾溶液(40∶65)为流动相,检测波长为230 nm。线性范围2.5~12.5μg,相关系数为0.99998。平均回收率为100.5%,RSD=2.2%(n=5)。结果:芍药苷在不同土壤条件下的平均含量分别为9.01%、5.56%、9.69%、8.23%、9.33%、6.22%、7.20%。结论:该方法准确,快速,重现性好,可作为赤芍栽培中内在质量评价的重要依据。     

Antioxidative constituents fromPaeonia lactiflora

The ethanol extract of the peony root (Paeonia Lactiflora Pall, Paeoniaceae) as well as its major active components including gallic acid and methyl gallate were evaluated for their protective effects against free radical generation and lipid peroxidation. In addition, the protective effects against hydrogen peroxide-induced oxidative DNA damage in a mammalian cell line were examined. The ethanol extracts of the peony root (PREs) and its active constituents, gallic acid and methyl gallate, exhibited a significant free radical scavenging effect against 1,1-diphenyl-2-picryl hydrazine (DPPH) radical generation and had an inhibitory effect on lipid peroxidation, as measured by the level of malondialdehyde (MDA) formation. The PREs did not have any pro-oxidant effect. They strongly inhibited the hydrogen peroxide-induced DNA damage from NIH/3T3 fibroblasts, as assessed by single cell gel electrophoresis. Furthermore, the oral administration of 50% PRE (50% ethanol extract of peony root), gallic acid and methyl gallate potently inhibited the formation of micronucleated reticulocytes (MNRET) in the mouse peripheral blood induced by a KBrO3 treatment in vivo. Therefore, PREs containing gallic acid and methyl gallate may be a useful antigenotoxic antioxidant by scavenging free radicals, inhibiting lipid peroxidation and protecting against oxidative DNA damage without exhibiting any pro-oxidant effect.     

A New antioxidant monoterpene glycoside, α-benzoyloxypaeoniflorin fromPaeonia suffruticosa

Alpha-benzoyloxypaeoniflorin (1), a new antioxidant monoterpene alpha-glycoside anomer was isolated from Paeonia suffruticosa along with known compounds, beta-benzoyloxypaeoniflorin (2), paeonolide, paeoniflorin and mudanpioside H. The structure of 1 has been determined by comparing spectral data with those of beta-benzoyloxypaeoniflorin (2). Compound 1 exhibited moderately potent radical scavenging activity on DPPH radical.     

Protective Effects for Hepalocytes of Salviae miltiorrhiza and Paeonia Iactiflora in Experimental Liver Damage

ＰｒｏｔｅｃｔｉｖｅＥｆｆｅｃｔｓｆｏｒＨｅｐａｌｏｃｙｔｅｓｏｆＳａｌｖｉａｅｍｉｌｔｉｏｒｒｈｉｚａａｎｄＰａｅｏｎｉａＩａｃｔｉｆｌｏｒａｉｎＥｘｐｅｒｉｍｅｎｔａｌＬｉｖｅｒＤａｍａｇｅＰｒｏｔｅｃｔｉｖｅＥｆｆｅｃｔｓｆｏｒＨｅｐａｌｏｃｙ...     

六味地黄丸中丹皮的质量研究

本文以不同种丹皮为对照,对六味地黄丸的原料药丹皮进行了鉴定;用无水乙醇提取、薄层扫描法测定了丹皮药材及六味地黄丸中丹皮酚的含量。本法与水蒸汽蒸馏-紫外分光光度法,醇浸出-紫外分光光度法作了比较,排除了杂质干扰,结果准确可靠。     

Protective effects for hepatocytes ofSalviae miltiorrhiza andPaeonia lactiflora in experimental liver damage

The protective effects for hepatocytes ofSalviae miltiorrhiza andPaeonia lactiflora in experimental rat models of liver damage were observed. Pathological changes in light and electron micro-scopies and plasma fibronectin level alterations of liver injury in rats, revealed thatSalviae miltiorrhiza could induce the increase of plasma fibronectin level in rats, thus improved phagocytosis of the reticuloendothelial system and opsonin activity, preventing the liver from immunologic damage, protecting hepatocytes and promoting regeneration. Similar but weaker actions were observed inPaeoniae lactiflora.