丹黄方对部分肝叶切除大鼠肝组织中Tec的影响

目的探讨丹黄方对部分肝叶切除大鼠肝组织中Tec mRNA表达的影响。方法采用部分肝叶切除肝再生模型。24只大鼠随机分为假手术组、手术组、丹黄方组和pHGF组。除假手术组外其余三组接受部分肝叶切除手术。从手术前3天至手术后48小时,丹黄方组给与丹黄方灌胃（10g/kg）及腹腔注射生理盐水（4．0ml/kg）,每天一次;促肝细胞生长素（pHGF）组给与生理盐水灌胃（4．0ml／kg）和腹腔注射pHGF（1ml/100g）,每天一次;手术组及假手术组仅给与生理盐水（4．0ml／kg）灌胃和腹腔注射。采用RT—PCR方法检测Tec mRNA的表达。结果丹黄方组肝组织中Tec mRNA的表达显著高于假手术组和手术组,P〈0．01;与pHGF组比较无显著差异,P〉0．05。结论丹黄方具有促进部分肝叶切除大鼠肝组织中Tec mRNA的表达的作用。     

中老年舒张性心力衰竭158例临床分析

目的对中老年舒张性心力衰竭（DHF）进行临床分析。方法回顾分析我院2007年1月至2010年2月收治的舒张性心力衰竭患者。结果舒张性心力衰竭早期心力衰竭症状较轻,可提前至中年发生,合并SHF（收缩性心力衰竭）者心力衰竭较重。结论彩色多普勒超声检查为评价DHF的主要方法,可对DHF进行分期,患者大多左房增大,部分患者左室及右房增大,LVEF正常或基本正常,舒张功能异常,左心型DHF占多数,少数患者合并右心型,综合治疗可延缓心室重构,改善心衰症状。     

The distribution of DEN infected people in Dushan and Xingyi area of Yunnan-Guizhou Plateau, China

The dengue viruses （DEN, genus Flavivirus, family Flaviviridae） are mosquito borne and have caused 100 million cases of dengue fever each year in most tropical and subtropical areas of the world. However, in the Southwest area of Yunnan-Guizhou Plateau, China, the previous work demonstrated that different geographic strains of Aedes albopictus were susceptible to dengue virus. In this study, we collected 456 sera samples from patients with fever and 994 sera samples from healthy population in Dushan and Xingyi area of Yunnan-Guizhou Plateau, China. All sera samples were tested for dengue IgG by enzyme-linked immunosorbent assay （ELISA）. Patients＇ sera samples were tested for dengue IgM and DEN antigen was checked in the sera of 6 from 456 samples with which C6/36 cell in culated by IFA. The results indicate that these patients with fever were infected with DEN-2 and suggest that DEN infection had existed in Dushan and Xingyi area of Yunnan-Guizhou Plateau, China.     

Infectious diseases in paediatric pathology: experience from a developing country

Infectious and parasitic diseases have always challenged man. Although many of them are typically seen in some areas of the world and can be adequately managed by just improving socioeconomic status and sanitary conditions, they are still quite prevalent and may sometimes be seen outside their original geographical areas. Human migration due to different reasons, tourism, blood transfusion and solid organ transplantation has created new concerns for health professionals all over the world. If not for diagnostic purposes, at least these tropical and infectious diseases should be largely known because their epidemiology, pathogenesis, host/parasite interaction, inflammatory and reparative responses are quite interesting and teach us about human biology. Curiosity is inherent to pathology practice and so we are compelled to look for things other than tumours or degenerative diseases. This review focuses on infectious and parasitic diseases found in a developing country and brings up-to-date information on diseases caused by viruses (dengue, yellow fever), bacteria (typhoid fever, leprosy), parasites (Chagas' disease, cutaneous and visceral leishmaniasis, amoebiasis, Capillaria hepatica, schistosomiasis, cysticercosis) and caused by fungi (paracoccidioidomycosis, cryptococcosis, histoplasmosis) that may be useful for pathologists when facing somewhat strange cases from developing countries.     

DHF-BAHPC molecule exerts ameliorative antioxidant status and reduced cadmium-induced toxicity in zebrafish (Danio rerio) embryos

In this study, we report the antioxidant and antitoxic potential of chemically synthesized 4-oxo-2-phenyl-4H-chromene-7,8-diyl bis((1-amino-2-hydroxypropyl)carbamate) (DHF-BAHPC) compound using in vitro and in vivo assays. The DHF-BAHPC was synthesized by linking 7,8-Dihydroxyflavone (DHF) with two molecules of Fmoc-threonine and characterized by Ultraviolet-visible spectroscopy (UV–vis), Fourier-transform infrared spectroscopy (FT-IR), ¹H NMR, ¹³C NMR and Mass spectrometry (MS). In vitro, antioxidant assay results revealed that DHF-BAHPC has a dose-dependent radical scavenging potential towards DPPH, ABTS, FRAP and H₂O₂ radicals with an IC₅₀ range of 15.45, 66.27, 25.71, 4.375 μg/mL, respectively. Furthermore DHF-BAHPC treatment significantly altered cadmium (Cd) intoxicated zebrafish embryos by rescuing the developmental changes associated with severe histological and reduced the level of defensive antioxidant activities (SOD, CAT, GPx and GST). The overall results of the present study represented that DHF-BAHPC may be used as a potential drug in redox-based therapeutics.     

登革病毒受体研究进展

登革病毒(DV)属于黄病毒属,分为4 个血清型,是具有包膜的单股正链RNA病毒。DV可通过埃及伊蚊和白纹伊蚊传播,引起登革热和登革出血热/登革休克综合征。该病广泛流行于热带和亚热带地区,是一种危害较大的传染病。DV感染机理尚不十分清楚,靶细胞上DV特异性受体的研究是近几年关注的热点,目前认为DV受体包括糖胺聚糖(GAGs)、硫酸乙酰肝素(HS)、与脂多糖连接的CD14相关分子等。现就DV受体的研究进展作一综述。     

A predictive model for Dengue Hemorrhagic Fever epidemics

A statistical model for predicting monthly Dengue Hemorrhagic Fever (DHF) cases from the city of Makassar is developed and tested. The model uses past and present DHF cases, climate and meteorological observations as inputs. These inputs are selected using a stepwise regression method to predict future DHF cases. The model is tested independently and its skill assessed using two skill measures. Using the selected variables as inputs, the model is capable of predicting a moderately-severe epidemic at lead times of up to six months. The most important input variable in the prediction is the present number of DHF cases followed by the relative humidity three to four months previously. A prediction 1-6 months in advance is sufficient to initiate various activities to combat DHF epidemic. The model is suitable for warning and easily becomes an operational tool due to its simplicity in data requirement and computational effort.     

舒张性心力衰竭临床诊治分析

目的：探讨舒张性心力衰竭的临床特点及诊治。方法：对2007年2月～2009年1月我院住院治疗的56例DHF患者的临床资料进行回顾性分析。结果：本组56例DHF患者经上述治疗后，症状完全消失、心功能I级以上49例（87．5％）；心功能I或Ⅱ级9例（16．1％）；5例（8．9％）合并右心衰竭和肾功能不全的患者的临床症状改善不明显，自动出院2例（3．6％），死亡1例（1．8％）。结论：患者存在心力衰竭的症状和体征，心脏超声示左心室舒张功能障碍，左心室射血分数≥50％，即可诊断为DHF；DHF的治疗以B受体阻滞剂、血管紧张素转换酶抑制剂（ACEI）、血管紧张素Ⅱ受体阻滞剂（ARB）以及钙通道拮抗剂为主，同时针对基础疾病采取对症治疗措施。     

Clinical implications and treatment of dengue

Dengue is a common pathogenic disease often proving fatal,more commonly affecting the tropics.Aedes mosquito is the vector for this disease,and outbreaks of dengue often cause mass damage to life.The current review is an effort to present an insight into the causes,etiology,symptoms,transmission,diagnosis,major organs affected,mitigation and line of treatment of this disease with special emphasis on dings of natural origin.The disease has a potential to spread as an endemic,often claiming several lives and thus requires concerted efforts to work out better treatment options.Traditional medicine offers an alternative solution and could be explored as a safer treatment option.Development of a successful vaccine and immunization technique largely remains a challenge and a better antiviral approach needs to be worked out to complement the supportive therapy.No single synthetic molecule has found to be wholly effective enough to offer curative control and the line of treatment mostly utilizes a combination of fluid replacement and antipyretics-analgesics like molecules to provide symptomatic relief.